Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis
Based on genetic diversity analysis with ISSR-PCR, this study was to access the germplasm resources of Platycodon grandiflorum in northern Anhui province. Ten primers that could produce more distinct and repeatable bands were used for ISSR-PCR. Statistic analysis was conducted by POPGENE v. 1.32, Ar...
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| Veröffentlicht in: | Molecular biology reports 2014-12, Vol.41 (12), p.8195-8201 |
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| creator | Nie, Chuanpeng Liu, Ruijiao Li, Shangbo Li, Yanyan |
| description | Based on genetic diversity analysis with ISSR-PCR, this study was to access the germplasm resources of
Platycodon grandiflorum
in northern Anhui province. Ten primers that could produce more distinct and repeatable bands were used for ISSR-PCR. Statistic analysis was conducted by POPGENE v. 1.32, Arlequin3.l, NTSYS-pc version 2.1. (1) Seventy-four polymorphic bands (76.29 %) out of a total of 97 were generated from 105 individuals in five populations. (2) Shannon index of diversity ranged from 0.307 to 0.260, diversity at species level was 0.3581, which means superior genetic diversity. (3) Genetic diversity across all the populations revealed by AMOVA indicated that 86.02 % occurred within populations. (4) The
Fst
value was 0.1398, indicating a intermediate genetic differentiation among populations. (5) Dendrogram relationship illustrated genetic distance was correlated with geographic distance. ISSR markers can be used for studying genetic diversity of
P. grandiflorum
. Degradation of populations doesn’t happen in northern Anhui province, bank of germplasm preservation should be established for cultivation of excellent variety of
P. grandiflorum. |
| doi_str_mv | 10.1007/s11033-014-3721-5 |
| format | Article |
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Platycodon grandiflorum
in northern Anhui province. Ten primers that could produce more distinct and repeatable bands were used for ISSR-PCR. Statistic analysis was conducted by POPGENE v. 1.32, Arlequin3.l, NTSYS-pc version 2.1. (1) Seventy-four polymorphic bands (76.29 %) out of a total of 97 were generated from 105 individuals in five populations. (2) Shannon index of diversity ranged from 0.307 to 0.260, diversity at species level was 0.3581, which means superior genetic diversity. (3) Genetic diversity across all the populations revealed by AMOVA indicated that 86.02 % occurred within populations. (4) The
Fst
value was 0.1398, indicating a intermediate genetic differentiation among populations. (5) Dendrogram relationship illustrated genetic distance was correlated with geographic distance. ISSR markers can be used for studying genetic diversity of
P. grandiflorum
. Degradation of populations doesn’t happen in northern Anhui province, bank of germplasm preservation should be established for cultivation of excellent variety of
P. grandiflorum.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-014-3721-5</identifier><identifier>PMID: 25200435</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; DNA, Plant - analysis ; Genetic diversity ; Genetic markers ; Genetic Variation ; Histology ; Life Sciences ; Microsatellite Repeats ; Morphology ; Phylogeny ; Plant populations ; Platycodon - genetics ; Platycodon - physiology ; Population genetics ; Seeds - genetics</subject><ispartof>Molecular biology reports, 2014-12, Vol.41 (12), p.8195-8201</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-36441839a92fee63670129ea32bd81a541842a13c379f3eead2eae3a7c2e0be43</citedby><cites>FETCH-LOGICAL-c372t-36441839a92fee63670129ea32bd81a541842a13c379f3eead2eae3a7c2e0be43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-014-3721-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-014-3721-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25200435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nie, Chuanpeng</creatorcontrib><creatorcontrib>Liu, Ruijiao</creatorcontrib><creatorcontrib>Li, Shangbo</creatorcontrib><creatorcontrib>Li, Yanyan</creatorcontrib><title>Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Based on genetic diversity analysis with ISSR-PCR, this study was to access the germplasm resources of
Platycodon grandiflorum
in northern Anhui province. Ten primers that could produce more distinct and repeatable bands were used for ISSR-PCR. Statistic analysis was conducted by POPGENE v. 1.32, Arlequin3.l, NTSYS-pc version 2.1. (1) Seventy-four polymorphic bands (76.29 %) out of a total of 97 were generated from 105 individuals in five populations. (2) Shannon index of diversity ranged from 0.307 to 0.260, diversity at species level was 0.3581, which means superior genetic diversity. (3) Genetic diversity across all the populations revealed by AMOVA indicated that 86.02 % occurred within populations. (4) The
Fst
value was 0.1398, indicating a intermediate genetic differentiation among populations. (5) Dendrogram relationship illustrated genetic distance was correlated with geographic distance. ISSR markers can be used for studying genetic diversity of
P. grandiflorum
. Degradation of populations doesn’t happen in northern Anhui province, bank of germplasm preservation should be established for cultivation of excellent variety of
P. grandiflorum.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>DNA, Plant - analysis</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Genetic Variation</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Microsatellite Repeats</subject><subject>Morphology</subject><subject>Phylogeny</subject><subject>Plant populations</subject><subject>Platycodon - genetics</subject><subject>Platycodon - physiology</subject><subject>Population genetics</subject><subject>Seeds - genetics</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1LHEEQhhuJ6PrxA3IJDbnkMrGrP7ZnjouYKAgRTc5N70yNjkx3b7pmhP33tlkNEsipCuqptz5exj6C-ApC2DMCEEpVAnSlrITK7LEFGKsq3dj6A1sIJaDStYFDdkT0KITQYM0BO5RGllyZBQsrIiQKGCeeen4z-mnbpi5Ffp997IZ-THkO_B5z2IyeAs9Iac4tEu9zCjymPD1gjnwVH-aBb3J6GmKLfO0JO15kru7ubrmPftzSQCdsv_cj4elrPGa_vl38PL-srn98vzpfXVdtuWOq1FJrqFXjG9kjLtXSCpANeiXXXQ3elKKWHlShm14h-k6iR-VtK1GsUatj9mWnW_b5PSNNLgzU4jj6iGkmB0sjtTHSqIJ-_gd9LPeVff9QQjW1bWyhYEe1ORFl7N0mD8HnrQPhXrxwOy9c8cK9eOFM6fn0qjyvA3Z_O96eXwC5A6iUYnnxu9H_VX0GqF-U1g</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Nie, Chuanpeng</creator><creator>Liu, Ruijiao</creator><creator>Li, Shangbo</creator><creator>Li, Yanyan</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20141201</creationdate><title>Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis</title><author>Nie, Chuanpeng ; Liu, Ruijiao ; Li, Shangbo ; Li, Yanyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-36441839a92fee63670129ea32bd81a541842a13c379f3eead2eae3a7c2e0be43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>DNA, Plant - analysis</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Genetic Variation</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Microsatellite Repeats</topic><topic>Morphology</topic><topic>Phylogeny</topic><topic>Plant populations</topic><topic>Platycodon - genetics</topic><topic>Platycodon - physiology</topic><topic>Population genetics</topic><topic>Seeds - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nie, Chuanpeng</creatorcontrib><creatorcontrib>Liu, Ruijiao</creatorcontrib><creatorcontrib>Li, Shangbo</creatorcontrib><creatorcontrib>Li, Yanyan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nie, Chuanpeng</au><au>Liu, Ruijiao</au><au>Li, Shangbo</au><au>Li, Yanyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>41</volume><issue>12</issue><spage>8195</spage><epage>8201</epage><pages>8195-8201</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Based on genetic diversity analysis with ISSR-PCR, this study was to access the germplasm resources of
Platycodon grandiflorum
in northern Anhui province. Ten primers that could produce more distinct and repeatable bands were used for ISSR-PCR. Statistic analysis was conducted by POPGENE v. 1.32, Arlequin3.l, NTSYS-pc version 2.1. (1) Seventy-four polymorphic bands (76.29 %) out of a total of 97 were generated from 105 individuals in five populations. (2) Shannon index of diversity ranged from 0.307 to 0.260, diversity at species level was 0.3581, which means superior genetic diversity. (3) Genetic diversity across all the populations revealed by AMOVA indicated that 86.02 % occurred within populations. (4) The
Fst
value was 0.1398, indicating a intermediate genetic differentiation among populations. (5) Dendrogram relationship illustrated genetic distance was correlated with geographic distance. ISSR markers can be used for studying genetic diversity of
P. grandiflorum
. Degradation of populations doesn’t happen in northern Anhui province, bank of germplasm preservation should be established for cultivation of excellent variety of
P. grandiflorum.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>25200435</pmid><doi>10.1007/s11033-014-3721-5</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular biology reports, 2014-12, Vol.41 (12), p.8195-8201 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences DNA, Plant - analysis Genetic diversity Genetic markers Genetic Variation Histology Life Sciences Microsatellite Repeats Morphology Phylogeny Plant populations Platycodon - genetics Platycodon - physiology Population genetics Seeds - genetics |
| title | Assessment of Platycodon grandiflorum germplasm resources from northern Anhui province based on ISSR analysis |
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