Radical-driven peptide backbone dissociation tandem mass spectrometry

In recent years, a number of novel tandem mass spectrometry approaches utilizing radical‐driven peptide gas‐phase fragmentation chemistry have been developed. These approaches show a peptide fragmentation pattern quite different from that of collision‐induced dissociation (CID). The peptide fragment...

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Veröffentlicht in:Mass spectrometry reviews 2015-03, Vol.34 (2), p.116-132
Hauptverfasser: Oh, Han Bin, Moon, Bongjin
Format: Artikel
Sprache:eng
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Zusammenfassung:In recent years, a number of novel tandem mass spectrometry approaches utilizing radical‐driven peptide gas‐phase fragmentation chemistry have been developed. These approaches show a peptide fragmentation pattern quite different from that of collision‐induced dissociation (CID). The peptide fragmentation features of these approaches share some in common with electron capture dissociation (ECD) or electron transfer dissociation (ETD) without the use of sophisticated equipment such as a Fourier‐transform mass spectrometer. For example, Siu and coworkers showed that CID of transition metal (ligand)‐peptide ternary complexes led to the formation of peptide radical ions through dissociative electron transfer (Chu et al., 2000. J Phys Chem B 104:3393–3397). The subsequent collisional activation of the generated radical ions resulted in a number of characteristic product ions, including a, c, x, z‐type fragments and notable side‐chain losses. Another example is the free radical initiated peptide sequencing (FRIPS) approach, in which Porter et al. and Beauchamp et al. independently introduced a free radical initiator to the primary amine group of the lysine side chain or N‐terminus of peptides (Masterson et al., 2004. J Am Chem Soc 126:720–721; Hodyss et al., 2005 J Am Chem Soc 127: 12436–12437). Photodetachment of gaseous multiply charged peptide anions (Joly et al., 2008. J Am Chem Soc 130:13832–13833) and UV photodissociation of photolabile radical precursors including a C–I bond (Ly & Julian, 2008. J Am Chem Soc 130:351–358; Ly & Julian, 2009. J Am Soc Mass Spectrom 20:1148–1158) also provide another route to generate radical ions. In this review, we provide a brief summary of recent results obtained through the radical‐driven peptide backbone dissociation tandem mass spectrometry approach. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 34: 116–132, 2015.
ISSN:0277-7037
1098-2787
DOI:10.1002/mas.21426