The effects of Acorus tatarinowii Schott on 5-HT concentrations, TPH2 and 5-HT1B expression in the dorsal raphe of exercised rats
Acorus tatarinowii Schott (Shi Chang Pu) belongs to the family of Acoraceae. The plant is used as an important herb for prolonging life many years in traditional Chinese medicine. It is an ancient herbal tonic nutriment and can be used as anti-fatigue medicine. However, the effects of Acorus tatarin...
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| Veröffentlicht in: | Journal of ethnopharmacology 2014-12, Vol.158, p.431-436 |
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| creator | Zhu, Meiju Zhu, Hongzhu Tan, Ninghua Zeng, Guangzhi Zeng, Zhigang Chu, Hongbiao Wang, Hui Xia, Zhi Wu, Renle |
| description | Acorus tatarinowii Schott (Shi Chang Pu) belongs to the family of Acoraceae. The plant is used as an important herb for prolonging life many years in traditional Chinese medicine. It is an ancient herbal tonic nutriment and can be used as anti-fatigue medicine. However, the effects of Acorus tatarinowii Schott on the endurance exercise in relation to central nervous system have not yet been clarified. In this study, the effects of Acorus tatarinowii Schott on treadmill running endurance, 5-HT concentrations, TPH2, 5-HT1B expression in the dorsal raphe of exercised rats were investigated.
Sixty adult male Sprague–Dawley rats were randomly divided into six groups: the normal group, the exercise group, the exercise and the rhizomes of Acorus tatarinowii Schott (ATS)(1mg/kg)-treated group, the exercise and ATS (10mg/kg)-treated group, the exercise and ATS (100mg/kg)-treated group, the exercise and caffeine (10mg/kg)-treated group. The effects of Acorus tatarinowii Schott on endurance exercise were determined by the time to exhaustion during treadmill exercise. The detection of 5-HT concentrations in the dorsal raphe was performed by HPLC analysis. The levels of TPH2, 5-HT1A and 5-HT1B expression were measured by western blot analysis and real-time PCR.
We found Acorus tatarinowii Schott could prolong the time to exhaustion in treadmill exercise and suppress the exercise-induced increase of 5-HT synthesis, TPH2 mRNA and protein expression and prevent the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe. Acorus tatarinowii Schott was as effective as caffeine in prolonging the exhaustion time in treadmill running and in decreasing the exercise-induced increase of 5-HT synthesis and TPH2 mRNA and protein expression and in preventing the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe.
The results indicated that the effects of Acorus tatarinowii Schott in inhibiting the exercise-induced synthesis of 5-HT and TPH2 expression and in preventing the exercise-induced decrease of 5-HT1B expression in the dorsal raphe might be the anti-fatigue mechanism of Acorus tatarinowii Schott.
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| doi_str_mv | 10.1016/j.jep.2014.10.026 |
| format | Article |
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Sixty adult male Sprague–Dawley rats were randomly divided into six groups: the normal group, the exercise group, the exercise and the rhizomes of Acorus tatarinowii Schott (ATS)(1mg/kg)-treated group, the exercise and ATS (10mg/kg)-treated group, the exercise and ATS (100mg/kg)-treated group, the exercise and caffeine (10mg/kg)-treated group. The effects of Acorus tatarinowii Schott on endurance exercise were determined by the time to exhaustion during treadmill exercise. The detection of 5-HT concentrations in the dorsal raphe was performed by HPLC analysis. The levels of TPH2, 5-HT1A and 5-HT1B expression were measured by western blot analysis and real-time PCR.
We found Acorus tatarinowii Schott could prolong the time to exhaustion in treadmill exercise and suppress the exercise-induced increase of 5-HT synthesis, TPH2 mRNA and protein expression and prevent the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe. Acorus tatarinowii Schott was as effective as caffeine in prolonging the exhaustion time in treadmill running and in decreasing the exercise-induced increase of 5-HT synthesis and TPH2 mRNA and protein expression and in preventing the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe.
The results indicated that the effects of Acorus tatarinowii Schott in inhibiting the exercise-induced synthesis of 5-HT and TPH2 expression and in preventing the exercise-induced decrease of 5-HT1B expression in the dorsal raphe might be the anti-fatigue mechanism of Acorus tatarinowii Schott.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2014.10.026</identifier><identifier>PMID: 25456438</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>5-hydroxytryptamine ; Acorus - chemistry ; Acorus tatarinowii Schott ; Animals ; Chromatography, High Pressure Liquid ; Dorsal Raphe Nucleus - drug effects ; Dorsal Raphe Nucleus - metabolism ; Exercise ; Male ; Physical Conditioning, Animal ; Plant Extracts - pharmacology ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction ; Receptor, Serotonin, 5-HT1B - metabolism ; Serotonergic inhibitory autoreceptor ; Serotonin - metabolism ; Tryptophan hydroxylase ; Tryptophan Hydroxylase - metabolism</subject><ispartof>Journal of ethnopharmacology, 2014-12, Vol.158, p.431-436</ispartof><rights>2014 Elsevier Ireland Ltd</rights><rights>Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-5bbaa5d9100659b122bb3e9d74deec5f6b6070cf21b88b5a5114461a527e5c1f3</citedby><cites>FETCH-LOGICAL-c419t-5bbaa5d9100659b122bb3e9d74deec5f6b6070cf21b88b5a5114461a527e5c1f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874114007363$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25456438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Meiju</creatorcontrib><creatorcontrib>Zhu, Hongzhu</creatorcontrib><creatorcontrib>Tan, Ninghua</creatorcontrib><creatorcontrib>Zeng, Guangzhi</creatorcontrib><creatorcontrib>Zeng, Zhigang</creatorcontrib><creatorcontrib>Chu, Hongbiao</creatorcontrib><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Xia, Zhi</creatorcontrib><creatorcontrib>Wu, Renle</creatorcontrib><title>The effects of Acorus tatarinowii Schott on 5-HT concentrations, TPH2 and 5-HT1B expression in the dorsal raphe of exercised rats</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Acorus tatarinowii Schott (Shi Chang Pu) belongs to the family of Acoraceae. The plant is used as an important herb for prolonging life many years in traditional Chinese medicine. It is an ancient herbal tonic nutriment and can be used as anti-fatigue medicine. However, the effects of Acorus tatarinowii Schott on the endurance exercise in relation to central nervous system have not yet been clarified. In this study, the effects of Acorus tatarinowii Schott on treadmill running endurance, 5-HT concentrations, TPH2, 5-HT1B expression in the dorsal raphe of exercised rats were investigated.
Sixty adult male Sprague–Dawley rats were randomly divided into six groups: the normal group, the exercise group, the exercise and the rhizomes of Acorus tatarinowii Schott (ATS)(1mg/kg)-treated group, the exercise and ATS (10mg/kg)-treated group, the exercise and ATS (100mg/kg)-treated group, the exercise and caffeine (10mg/kg)-treated group. The effects of Acorus tatarinowii Schott on endurance exercise were determined by the time to exhaustion during treadmill exercise. The detection of 5-HT concentrations in the dorsal raphe was performed by HPLC analysis. The levels of TPH2, 5-HT1A and 5-HT1B expression were measured by western blot analysis and real-time PCR.
We found Acorus tatarinowii Schott could prolong the time to exhaustion in treadmill exercise and suppress the exercise-induced increase of 5-HT synthesis, TPH2 mRNA and protein expression and prevent the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe. Acorus tatarinowii Schott was as effective as caffeine in prolonging the exhaustion time in treadmill running and in decreasing the exercise-induced increase of 5-HT synthesis and TPH2 mRNA and protein expression and in preventing the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe.
The results indicated that the effects of Acorus tatarinowii Schott in inhibiting the exercise-induced synthesis of 5-HT and TPH2 expression and in preventing the exercise-induced decrease of 5-HT1B expression in the dorsal raphe might be the anti-fatigue mechanism of Acorus tatarinowii Schott.
[Display omitted]</description><subject>5-hydroxytryptamine</subject><subject>Acorus - chemistry</subject><subject>Acorus tatarinowii Schott</subject><subject>Animals</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dorsal Raphe Nucleus - drug effects</subject><subject>Dorsal Raphe Nucleus - metabolism</subject><subject>Exercise</subject><subject>Male</subject><subject>Physical Conditioning, Animal</subject><subject>Plant Extracts - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptor, Serotonin, 5-HT1B - metabolism</subject><subject>Serotonergic inhibitory autoreceptor</subject><subject>Serotonin - metabolism</subject><subject>Tryptophan hydroxylase</subject><subject>Tryptophan Hydroxylase - metabolism</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9PGzEQxa2qqKTQD8AF-dgDGzxee70RJ4raphJSK5GeLf-ZFY6S9WI7hR77zes0wLGn0Zt580bzI-QM2BwYdJfr-RqnOWcgqp4z3r0hM-gVb5RU7VsyY63qm14JOCbvc14zxhQI9o4ccylkJ9p-Rv6s7pHiMKArmcaBXruYdpkWU0wKY3wMgd65-1gKjSOVzXJFXRwdjiWZEuKYL-jqx5JTM_p_U_hE8WlKmHMd0jDSUuN9TNlsaDJTFfUGPmFyIaOvrZJPydFgNhk_PNcT8vPL59XNsrn9_vXbzfVt4wQsSiOtNUb6BTDWyYUFzq1tceGV8IhODp3tmGJu4GD73kojAYTowEiuUDoY2hPy8ZA7pfiww1z0NmSHm40ZMe6yhk7yiqTt-2qFg9WlmHPCQU8pbE36rYHpPXm91pW83pPftyr5unP-HL-zW_SvGy-oq-HqYMD65K-ASWcXsLL0IVX62sfwn_i_4iWTgg</recordid><startdate>20141202</startdate><enddate>20141202</enddate><creator>Zhu, Meiju</creator><creator>Zhu, Hongzhu</creator><creator>Tan, Ninghua</creator><creator>Zeng, Guangzhi</creator><creator>Zeng, Zhigang</creator><creator>Chu, Hongbiao</creator><creator>Wang, Hui</creator><creator>Xia, Zhi</creator><creator>Wu, Renle</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141202</creationdate><title>The effects of Acorus tatarinowii Schott on 5-HT concentrations, TPH2 and 5-HT1B expression in the dorsal raphe of exercised rats</title><author>Zhu, Meiju ; Zhu, Hongzhu ; Tan, Ninghua ; Zeng, Guangzhi ; Zeng, Zhigang ; Chu, Hongbiao ; Wang, Hui ; Xia, Zhi ; Wu, Renle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-5bbaa5d9100659b122bb3e9d74deec5f6b6070cf21b88b5a5114461a527e5c1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>5-hydroxytryptamine</topic><topic>Acorus - chemistry</topic><topic>Acorus tatarinowii Schott</topic><topic>Animals</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dorsal Raphe Nucleus - drug effects</topic><topic>Dorsal Raphe Nucleus - metabolism</topic><topic>Exercise</topic><topic>Male</topic><topic>Physical Conditioning, Animal</topic><topic>Plant Extracts - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptor, Serotonin, 5-HT1B - metabolism</topic><topic>Serotonergic inhibitory autoreceptor</topic><topic>Serotonin - metabolism</topic><topic>Tryptophan hydroxylase</topic><topic>Tryptophan Hydroxylase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Meiju</creatorcontrib><creatorcontrib>Zhu, Hongzhu</creatorcontrib><creatorcontrib>Tan, Ninghua</creatorcontrib><creatorcontrib>Zeng, Guangzhi</creatorcontrib><creatorcontrib>Zeng, Zhigang</creatorcontrib><creatorcontrib>Chu, Hongbiao</creatorcontrib><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Xia, Zhi</creatorcontrib><creatorcontrib>Wu, Renle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Meiju</au><au>Zhu, Hongzhu</au><au>Tan, Ninghua</au><au>Zeng, Guangzhi</au><au>Zeng, Zhigang</au><au>Chu, Hongbiao</au><au>Wang, Hui</au><au>Xia, Zhi</au><au>Wu, Renle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of Acorus tatarinowii Schott on 5-HT concentrations, TPH2 and 5-HT1B expression in the dorsal raphe of exercised rats</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2014-12-02</date><risdate>2014</risdate><volume>158</volume><spage>431</spage><epage>436</epage><pages>431-436</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Acorus tatarinowii Schott (Shi Chang Pu) belongs to the family of Acoraceae. The plant is used as an important herb for prolonging life many years in traditional Chinese medicine. It is an ancient herbal tonic nutriment and can be used as anti-fatigue medicine. However, the effects of Acorus tatarinowii Schott on the endurance exercise in relation to central nervous system have not yet been clarified. In this study, the effects of Acorus tatarinowii Schott on treadmill running endurance, 5-HT concentrations, TPH2, 5-HT1B expression in the dorsal raphe of exercised rats were investigated.
Sixty adult male Sprague–Dawley rats were randomly divided into six groups: the normal group, the exercise group, the exercise and the rhizomes of Acorus tatarinowii Schott (ATS)(1mg/kg)-treated group, the exercise and ATS (10mg/kg)-treated group, the exercise and ATS (100mg/kg)-treated group, the exercise and caffeine (10mg/kg)-treated group. The effects of Acorus tatarinowii Schott on endurance exercise were determined by the time to exhaustion during treadmill exercise. The detection of 5-HT concentrations in the dorsal raphe was performed by HPLC analysis. The levels of TPH2, 5-HT1A and 5-HT1B expression were measured by western blot analysis and real-time PCR.
We found Acorus tatarinowii Schott could prolong the time to exhaustion in treadmill exercise and suppress the exercise-induced increase of 5-HT synthesis, TPH2 mRNA and protein expression and prevent the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe. Acorus tatarinowii Schott was as effective as caffeine in prolonging the exhaustion time in treadmill running and in decreasing the exercise-induced increase of 5-HT synthesis and TPH2 mRNA and protein expression and in preventing the exercise-induced decrease of 5-HT1B mRNA and protein expression in the dorsal raphe.
The results indicated that the effects of Acorus tatarinowii Schott in inhibiting the exercise-induced synthesis of 5-HT and TPH2 expression and in preventing the exercise-induced decrease of 5-HT1B expression in the dorsal raphe might be the anti-fatigue mechanism of Acorus tatarinowii Schott.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>25456438</pmid><doi>10.1016/j.jep.2014.10.026</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of ethnopharmacology, 2014-12, Vol.158, p.431-436 |
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| subjects | 5-hydroxytryptamine Acorus - chemistry Acorus tatarinowii Schott Animals Chromatography, High Pressure Liquid Dorsal Raphe Nucleus - drug effects Dorsal Raphe Nucleus - metabolism Exercise Male Physical Conditioning, Animal Plant Extracts - pharmacology Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Receptor, Serotonin, 5-HT1B - metabolism Serotonergic inhibitory autoreceptor Serotonin - metabolism Tryptophan hydroxylase Tryptophan Hydroxylase - metabolism |
| title | The effects of Acorus tatarinowii Schott on 5-HT concentrations, TPH2 and 5-HT1B expression in the dorsal raphe of exercised rats |
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