HPLC analysis of blood–brain barrier penetration of 4-fluorodeprenyl
•A reversed-phase HPLC method was developed and validated for the determination of 4-fluorodeprenyl.•The level of 4-fluorodeprenyl was monitored in various body compartments of rats.•Definite blood–brain barrier penetration of 4-fluorodeprenyl was stated. Validated HPLC analysis was developed in ord...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2015-01, Vol.102, p.529-534 |
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| creator | Pöstényi, Zita Tekes, Kornélia Tóth-Molnár, Edit Kalász, Huba |
| description | •A reversed-phase HPLC method was developed and validated for the determination of 4-fluorodeprenyl.•The level of 4-fluorodeprenyl was monitored in various body compartments of rats.•Definite blood–brain barrier penetration of 4-fluorodeprenyl was stated.
Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30mg/kg of (−)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24μg/g, showing maximum concentration in the brain and the liver after 5min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland. |
| doi_str_mv | 10.1016/j.jpba.2014.09.035 |
| format | Article |
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Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30mg/kg of (−)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24μg/g, showing maximum concentration in the brain and the liver after 5min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2014.09.035</identifier><identifier>PMID: 25459953</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>(−)-4-Fluorodeprenyl ; Animals ; Biological Transport - physiology ; Blood-Brain Barrier - drug effects ; Blood-Brain Barrier - metabolism ; Blood–Brain barrier (BBB) ; Brain - drug effects ; Brain - metabolism ; Chemistry Techniques, Analytical - methods ; Chromatography, High Pressure Liquid - methods ; HPLC ; Lacrimal gland ; Male ; Rats ; Rats, Wistar ; Selegiline - analogs & derivatives ; Selegiline - analysis ; Selegiline - metabolism ; Selegiline - pharmacology</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2015-01, Vol.102, p.529-534</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-a519882f18042bc65abe09aa68c23683322ef164fc662b5e105a2660ac6715af3</citedby><cites>FETCH-LOGICAL-c400t-a519882f18042bc65abe09aa68c23683322ef164fc662b5e105a2660ac6715af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708514004877$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25459953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pöstényi, Zita</creatorcontrib><creatorcontrib>Tekes, Kornélia</creatorcontrib><creatorcontrib>Tóth-Molnár, Edit</creatorcontrib><creatorcontrib>Kalász, Huba</creatorcontrib><title>HPLC analysis of blood–brain barrier penetration of 4-fluorodeprenyl</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>•A reversed-phase HPLC method was developed and validated for the determination of 4-fluorodeprenyl.•The level of 4-fluorodeprenyl was monitored in various body compartments of rats.•Definite blood–brain barrier penetration of 4-fluorodeprenyl was stated.
Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30mg/kg of (−)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24μg/g, showing maximum concentration in the brain and the liver after 5min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.</description><subject>(−)-4-Fluorodeprenyl</subject><subject>Animals</subject><subject>Biological Transport - physiology</subject><subject>Blood-Brain Barrier - drug effects</subject><subject>Blood-Brain Barrier - metabolism</subject><subject>Blood–Brain barrier (BBB)</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Chemistry Techniques, Analytical - methods</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>HPLC</subject><subject>Lacrimal gland</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Selegiline - analogs & derivatives</subject><subject>Selegiline - analysis</subject><subject>Selegiline - metabolism</subject><subject>Selegiline - pharmacology</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1Kw0AUhQdRbP15AReSpZvEO79JwI0UtUJBFwruhsnkBqakmTiTCt35Dr6hT2JKq0tXd_Odc7gfIRcUMgpUXS-zZV-ZjAEVGZQZcHlAprTIecqUeDskU8g5TXMo5IScxLgEAElLcUwmTApZlpJPyf38eTFLTGfaTXQx8U1Std7X359fVTCuSyoTgsOQ9NjhEMzgfLeFRNq0ax98jX3AbtOekaPGtBHP9_eUvN7fvczm6eLp4XF2u0itABhSM-4XBWtoAYJVVklTIZTGqMIyrgrOGcOGKtFYpVglkYI0TCkwVuVUmoafkqtdbx_8-xrjoFcuWmxb06FfR02VZILnY2RE2Q61wccYsNF9cCsTNpqC3vrTS731p7f-NJR69DeGLvf962qF9V_kV9gI3OwAHL_8GM3oaB12FmsX0A669u6__h8aAYEt</recordid><startdate>20150105</startdate><enddate>20150105</enddate><creator>Pöstényi, Zita</creator><creator>Tekes, Kornélia</creator><creator>Tóth-Molnár, Edit</creator><creator>Kalász, Huba</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150105</creationdate><title>HPLC analysis of blood–brain barrier penetration of 4-fluorodeprenyl</title><author>Pöstényi, Zita ; Tekes, Kornélia ; Tóth-Molnár, Edit ; Kalász, Huba</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-a519882f18042bc65abe09aa68c23683322ef164fc662b5e105a2660ac6715af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>(−)-4-Fluorodeprenyl</topic><topic>Animals</topic><topic>Biological Transport - physiology</topic><topic>Blood-Brain Barrier - drug effects</topic><topic>Blood-Brain Barrier - metabolism</topic><topic>Blood–Brain barrier (BBB)</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Chemistry Techniques, Analytical - methods</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>HPLC</topic><topic>Lacrimal gland</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Selegiline - analogs & derivatives</topic><topic>Selegiline - analysis</topic><topic>Selegiline - metabolism</topic><topic>Selegiline - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pöstényi, Zita</creatorcontrib><creatorcontrib>Tekes, Kornélia</creatorcontrib><creatorcontrib>Tóth-Molnár, Edit</creatorcontrib><creatorcontrib>Kalász, Huba</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pöstényi, Zita</au><au>Tekes, Kornélia</au><au>Tóth-Molnár, Edit</au><au>Kalász, Huba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPLC analysis of blood–brain barrier penetration of 4-fluorodeprenyl</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2015-01-05</date><risdate>2015</risdate><volume>102</volume><spage>529</spage><epage>534</epage><pages>529-534</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><abstract>•A reversed-phase HPLC method was developed and validated for the determination of 4-fluorodeprenyl.•The level of 4-fluorodeprenyl was monitored in various body compartments of rats.•Definite blood–brain barrier penetration of 4-fluorodeprenyl was stated.
Validated HPLC analysis was developed in order to monitor the level of 4-fluorodeprenyl in rats. Male Wistar rats were intraperitoneally treated with 30mg/kg of (−)-4-fluorodeprenyl. The rats were sacrificed after 5, 15, 30 and 60min of treatment, and various tissues were isolated, such as serum, brain, CSF, liver, testis and lacrimal gland. Perchloric acid was given to aliquots, which were then homogenized, centrifuged and the supernatants were taken. The 4-fluorodeprenyl content was determined using reversed-phase HPLC, based on the comparison of the calibration line of the spiked samples. The level of 4-fluorodeprenyl was between 0.5 and 24μg/g, showing maximum concentration in the brain and the liver after 5min following its administration and in serum, CSF, testis, eyes and lacrimal gland after 15min following its administration, while a relatively high concentration was found in the liver and the lacrimal gland.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25459953</pmid><doi>10.1016/j.jpba.2014.09.035</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of pharmaceutical and biomedical analysis, 2015-01, Vol.102, p.529-534 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | (−)-4-Fluorodeprenyl Animals Biological Transport - physiology Blood-Brain Barrier - drug effects Blood-Brain Barrier - metabolism Blood–Brain barrier (BBB) Brain - drug effects Brain - metabolism Chemistry Techniques, Analytical - methods Chromatography, High Pressure Liquid - methods HPLC Lacrimal gland Male Rats Rats, Wistar Selegiline - analogs & derivatives Selegiline - analysis Selegiline - metabolism Selegiline - pharmacology |
| title | HPLC analysis of blood–brain barrier penetration of 4-fluorodeprenyl |
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