Sterility Testing of Stem Cell Products by Broad-Range Bacterial 16S Ribosomal DNA Polymerase Chain Reaction

Objective: To evaluate broad-range 16S ribosomal DNA (rDNA) polymerase chain reaction (PCR) as a rapid screening tool to detect bacterial contamination of stem-cell products. Methods: We performed the evaluation using whole blood spiked with serially diluted bacterial-type strains. Detection sensiti...

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Veröffentlicht in:	Laboratory medicine 2015-02, Vol.46 (1), p.34-41
Hauptverfasser:	Tokuno, Osamu, Hayakawa, Akira, Yanai, Tomoko, Mori, Takeshi, Ohnuma, Kenichiro, Tani, Ayumi, Minami, Hironobu, Sugimoto, Takeshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Deoxyribonucleic acid DNA DNA, Bacterial - analysis Gene amplification Humans Infertility Medicine Methods Process controls RNA, Messenger - metabolism RNA, Ribosomal, 16S - metabolism Stem cells Stem Cells - metabolism
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