Vitamin A is a key regulator for cell growth, cytokine production, and differentiation in normal B cells

In the present paper we demonstrate that retinol-retinol-binding protein and chylomicron remnant retinyl esters in concentrations normally found in human plasma inhibit growth of normal human B lymphocytes. Physiological concentrations of retinoic acid (about 30 nM) were less active than physiologic...

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Veröffentlicht in:The Journal of biological chemistry 1992-11, Vol.267 (33), p.23988-23992
Hauptverfasser: Blomhoff, H K, Smeland, E B, Erikstein, B, Rasmussen, A M, Skrede, B, Skjønsberg, C, Blomhoff, R
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container_end_page 23992
container_issue 33
container_start_page 23988
container_title The Journal of biological chemistry
container_volume 267
creator Blomhoff, H K
Smeland, E B
Erikstein, B
Rasmussen, A M
Skrede, B
Skjønsberg, C
Blomhoff, R
description In the present paper we demonstrate that retinol-retinol-binding protein and chylomicron remnant retinyl esters in concentrations normally found in human plasma inhibit growth of normal human B lymphocytes. Physiological concentrations of retinoic acid (about 30 nM) were less active than physiological concentrations of retinol (about 3 microM). Pharmacological concentrations of retinol and retinoic acid were more active than the concentrations normally found in plasma. Retinol (3 microM) inhibited anti-IgM-mediated DNA synthesis as measured by [3H]thymidine uptake at 72 h by 78%. Furthermore, we found that the cells were blocked in the mid-G1 phase of the cell cycle. Thus, neither MYC up-regulation measured at 3 h nor the expression of the early activation antigen 4F2 was reduced by retinol, whereas the late activation markers (transferrin receptor expression and actinomycin D staining at 48 h of stimulation) were markedly inhibited. Retinol reduced the interleukin 6 production induced by anti-IgM and interleukin 4 after 48 h, whereas the induction of interleukin 6 and tumor necrosis factor by O-tetradecanoylphorbol-13-acetate and ionomycin was less affected. We also noted that the retinoids reduced the formation of plaque-forming cells (i.e. Ig synthesis). These data imply that vitamin A present in human plasma is a normal modulator of B cell function.
doi_str_mv 10.1016/S0021-9258(18)35934-9
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ispartof The Journal of biological chemistry, 1992-11, Vol.267 (33), p.23988-23992
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source MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects B-Lymphocytes - cytology
B-Lymphocytes - drug effects
B-Lymphocytes - physiology
Cell Cycle - drug effects
Cell Differentiation - drug effects
Cell Division - drug effects
Cells, Cultured
Chylomicrons - pharmacology
Cytokines - biosynthesis
DNA - biosynthesis
Dose-Response Relationship, Drug
Humans
Interleukin-6 - biosynthesis
Kinetics
Thymidine - metabolism
Tretinoin - pharmacology
Tritium
Tumor Necrosis Factor-alpha - biosynthesis
Vitamin A - pharmacology
title Vitamin A is a key regulator for cell growth, cytokine production, and differentiation in normal B cells
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