Cold-sensitive E-lysis systems
The release of recombinant bacteria into the environment is undesirable because of possible risks associated with the genetically modified organisms. The aim of this study was to establish a cold-sensitive killing system with a lethal gene, activated when bacteria encounter lower environmental tempe...
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| Veröffentlicht in: | Gene 1998-09, Vol.218 (1), p.1-7 |
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| container_title | Gene |
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| creator | Jechlinger, Wolfgang Szostak, Michael P. Lubitz, Werner |
| description | The release of recombinant bacteria into the environment is undesirable because of possible risks associated with the genetically modified organisms. The aim of this study was to establish a cold-sensitive killing system with a lethal gene, activated when bacteria encounter lower environmental temperatures. To obtain cold-sensitive lysis vectors, the
λcI857 repressor/
p
R promoter expression system was combined with either the
lacI/
lacZpo or the phage 434
cI/
p
R system that control the expression of the lysis gene
E of bacteriophage
φX174.
Escherichia coli strains harbouring such suicide vectors are able to grow at 37°C, but cell lysis takes place at temperatures below 30°C. By replacing gene
E with a
β-galactosidase reporter gene we also showed that the onset of
β-galactosidase activity corresponds with the onset of lysis at 28°C. Results indicate that these newly combined promoter/repressor systems can also be used to confer cold-sensitive expression to any gene of interest. |
| doi_str_mv | 10.1016/S0378-1119(98)00405-3 |
| format | Article |
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λcI857 repressor/
p
R promoter expression system was combined with either the
lacI/
lacZpo or the phage 434
cI/
p
R system that control the expression of the lysis gene
E of bacteriophage
φX174.
Escherichia coli strains harbouring such suicide vectors are able to grow at 37°C, but cell lysis takes place at temperatures below 30°C. By replacing gene
E with a
β-galactosidase reporter gene we also showed that the onset of
β-galactosidase activity corresponds with the onset of lysis at 28°C. Results indicate that these newly combined promoter/repressor systems can also be used to confer cold-sensitive expression to any gene of interest.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(98)00405-3</identifier><identifier>PMID: 9751796</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacterial Proteins - genetics ; Bacteriophage phi X 174 - genetics ; beta-Galactosidase - genetics ; Cold Temperature ; Escherichia coli - genetics ; Escherichia coli Proteins ; Gene expression ; Gene Expression Regulation ; Genes, Reporter ; Genetic Engineering ; Genetic Vectors ; Lac Operon ; Lac Repressors ; Live vaccines ; Mutagenesis ; PhiX174 gene E ; Promoter Regions, Genetic ; Repressor Proteins - genetics ; Safety cassette ; Viral Proteins - genetics</subject><ispartof>Gene, 1998-09, Vol.218 (1), p.1-7</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-a3a8e2325f686ef0239aea23e759496075cd44efdaf843750f6380abece9806c3</citedby><cites>FETCH-LOGICAL-c391t-a3a8e2325f686ef0239aea23e759496075cd44efdaf843750f6380abece9806c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(98)00405-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9751796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jechlinger, Wolfgang</creatorcontrib><creatorcontrib>Szostak, Michael P.</creatorcontrib><creatorcontrib>Lubitz, Werner</creatorcontrib><title>Cold-sensitive E-lysis systems</title><title>Gene</title><addtitle>Gene</addtitle><description>The release of recombinant bacteria into the environment is undesirable because of possible risks associated with the genetically modified organisms. The aim of this study was to establish a cold-sensitive killing system with a lethal gene, activated when bacteria encounter lower environmental temperatures. To obtain cold-sensitive lysis vectors, the
λcI857 repressor/
p
R promoter expression system was combined with either the
lacI/
lacZpo or the phage 434
cI/
p
R system that control the expression of the lysis gene
E of bacteriophage
φX174.
Escherichia coli strains harbouring such suicide vectors are able to grow at 37°C, but cell lysis takes place at temperatures below 30°C. By replacing gene
E with a
β-galactosidase reporter gene we also showed that the onset of
β-galactosidase activity corresponds with the onset of lysis at 28°C. Results indicate that these newly combined promoter/repressor systems can also be used to confer cold-sensitive expression to any gene of interest.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacteriophage phi X 174 - genetics</subject><subject>beta-Galactosidase - genetics</subject><subject>Cold Temperature</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Proteins</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes, Reporter</subject><subject>Genetic Engineering</subject><subject>Genetic Vectors</subject><subject>Lac Operon</subject><subject>Lac Repressors</subject><subject>Live vaccines</subject><subject>Mutagenesis</subject><subject>PhiX174 gene E</subject><subject>Promoter Regions, Genetic</subject><subject>Repressor Proteins - genetics</subject><subject>Safety cassette</subject><subject>Viral Proteins - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUMtKAzEUDaLUWv2ESleii-jNZJJJViKlPqDgQl2HNHMDkZlOnTst9O-dPnDr3dzFeXEOY2MB9wKEfvgAWRguhLC31twB5KC4PGFDYQrLAaQ5ZcM_yjm7IPqG_pTKBmxgCyUKq4fsetpUJSdcUurSBiczXm0p0YS21GFNl-ws-orw6vhH7Ot59jl95fP3l7fp05wHaUXHvfQGM5mpqI3GCJm0Hn0msVA2txoKFco8x1j6aHJZKIhaGvALDGgN6CBH7Obgu2qbnzVS5-pEAavKL7FZkxNaZdB79UR1IIa2IWoxulWbat9unQC328Xtd3G70s4at9_FyV43PgasFzWWf6rjED3-eMCxb7lJ2DoKCZcBy9Ri6FzZpH8SfgE00HB9</recordid><startdate>19980918</startdate><enddate>19980918</enddate><creator>Jechlinger, Wolfgang</creator><creator>Szostak, Michael P.</creator><creator>Lubitz, Werner</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19980918</creationdate><title>Cold-sensitive E-lysis systems</title><author>Jechlinger, Wolfgang ; Szostak, Michael P. ; Lubitz, Werner</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-a3a8e2325f686ef0239aea23e759496075cd44efdaf843750f6380abece9806c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacteriophage phi X 174 - genetics</topic><topic>beta-Galactosidase - genetics</topic><topic>Cold Temperature</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Proteins</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes, Reporter</topic><topic>Genetic Engineering</topic><topic>Genetic Vectors</topic><topic>Lac Operon</topic><topic>Lac Repressors</topic><topic>Live vaccines</topic><topic>Mutagenesis</topic><topic>PhiX174 gene E</topic><topic>Promoter Regions, Genetic</topic><topic>Repressor Proteins - genetics</topic><topic>Safety cassette</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jechlinger, Wolfgang</creatorcontrib><creatorcontrib>Szostak, Michael P.</creatorcontrib><creatorcontrib>Lubitz, Werner</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jechlinger, Wolfgang</au><au>Szostak, Michael P.</au><au>Lubitz, Werner</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cold-sensitive E-lysis systems</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1998-09-18</date><risdate>1998</risdate><volume>218</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The release of recombinant bacteria into the environment is undesirable because of possible risks associated with the genetically modified organisms. The aim of this study was to establish a cold-sensitive killing system with a lethal gene, activated when bacteria encounter lower environmental temperatures. To obtain cold-sensitive lysis vectors, the
λcI857 repressor/
p
R promoter expression system was combined with either the
lacI/
lacZpo or the phage 434
cI/
p
R system that control the expression of the lysis gene
E of bacteriophage
φX174.
Escherichia coli strains harbouring such suicide vectors are able to grow at 37°C, but cell lysis takes place at temperatures below 30°C. By replacing gene
E with a
β-galactosidase reporter gene we also showed that the onset of
β-galactosidase activity corresponds with the onset of lysis at 28°C. Results indicate that these newly combined promoter/repressor systems can also be used to confer cold-sensitive expression to any gene of interest.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9751796</pmid><doi>10.1016/S0378-1119(98)00405-3</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1998-09, Vol.218 (1), p.1-7 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16520594 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacterial Proteins - genetics Bacteriophage phi X 174 - genetics beta-Galactosidase - genetics Cold Temperature Escherichia coli - genetics Escherichia coli Proteins Gene expression Gene Expression Regulation Genes, Reporter Genetic Engineering Genetic Vectors Lac Operon Lac Repressors Live vaccines Mutagenesis PhiX174 gene E Promoter Regions, Genetic Repressor Proteins - genetics Safety cassette Viral Proteins - genetics |
| title | Cold-sensitive E-lysis systems |
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