Effects of mutations in the starch-binding domain of Bacillus macerans cyclodextrin glycosyltransferase

Cyclodextrin glycosyltransferase (CGTase) is an industrially important enzyme that produces cyclodextrins (CD) from starch by intramolecular transglycosylation. CGTase consists of five globular domains labeled A through E. To better understand the role of domain E in CGTase catalysis, we have constr...

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Veröffentlicht in:	Journal of biotechnology 1998-10, Vol.65 (2), p.191-202
Hauptverfasser:	Chang, Hai-yin, Irwin, Philip M, Nikolov, Zivko L
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus - enzymology Bacillus - genetics Biological and medical sciences Biotechnology Blotting, Western CGTase Cyclodextrin glycosyltransferase Cyclodextrins Cyclodextrins - metabolism Electrophoresis, Polyacrylamide Gel Enzyme Stability Fundamental and applied biological sciences. Psychology Glucosyltransferases - chemistry Glucosyltransferases - genetics Glucosyltransferases - isolation & purification Glucosyltransferases - metabolism Methods. Procedures. Technologies Mutagenesis, Site-Directed Plasmids - genetics Protein engineering Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Starch - metabolism Starch-binding domain Structure-Activity Relationship Temperature
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