Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes
Abstract Toxic strains of Microcystis aeruginosa produce cyclic heptatoxins (microcystins) that are believed to be synthesized non-ribosomally by peptide synthetases. We analysed toxin-producing and non-toxic strains of M. aeruginosa with respect to the presence of DNA sequences potentially encoding...
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| Veröffentlicht in: | FEMS microbiology letters 1996-01, Vol.135 (2-3), p.295-303 |
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| creator | Meiβner, Kathrin Dittmann, Elke Börner, Thomas |
| description | Abstract
Toxic strains of Microcystis aeruginosa produce cyclic heptatoxins (microcystins) that are believed to be synthesized non-ribosomally by peptide synthetases. We analysed toxin-producing and non-toxic strains of M. aeruginosa with respect to the presence of DNA sequences potentially encoding peptide synthetases. Hybridizations of genomic DNA of various M. aeruginosa strains with PCR-amplificated fragments possessing homologies to adenylate-forming domains of peptide synthetase genes provided first evidence for the existence of corresponding genes in cyanobacteria. Furthermore we isolated and sequenced from genomic libraries overlapping fragments of M. aeruginosa DNA with a total length of 2982 bp showing significant homology to genes encoding peptide synthetases and hybridizing exclusively with DNA from toxic strains. Our results indicate that both toxic and non-toxic strains of M. aeruginosa possess genes coding for peptide synthetases and that hepatotoxin-producing and non-toxic strains differ in their content of genes for specific peptide synthetases. |
| doi_str_mv | 10.1111/j.1574-6968.1996.tb08004.x |
| format | Article |
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Toxic strains of Microcystis aeruginosa produce cyclic heptatoxins (microcystins) that are believed to be synthesized non-ribosomally by peptide synthetases. We analysed toxin-producing and non-toxic strains of M. aeruginosa with respect to the presence of DNA sequences potentially encoding peptide synthetases. Hybridizations of genomic DNA of various M. aeruginosa strains with PCR-amplificated fragments possessing homologies to adenylate-forming domains of peptide synthetase genes provided first evidence for the existence of corresponding genes in cyanobacteria. Furthermore we isolated and sequenced from genomic libraries overlapping fragments of M. aeruginosa DNA with a total length of 2982 bp showing significant homology to genes encoding peptide synthetases and hybridizing exclusively with DNA from toxic strains. Our results indicate that both toxic and non-toxic strains of M. aeruginosa possess genes coding for peptide synthetases and that hepatotoxin-producing and non-toxic strains differ in their content of genes for specific peptide synthetases.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1996.tb08004.x</identifier><identifier>PMID: 8595871</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Toxins ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Conserved Sequence - genetics ; Cyanobacteria ; Cyanophyta ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; Domains ; Fragments ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gene sequencing ; Genes ; Genes, Bacterial - genetics ; Genetics ; Hepatotoxicity ; Homology ; Microbiology ; Microcysti ; Microcystins ; Microcystis ; Microcystis - enzymology ; Microcystis - genetics ; Microcystis aeruginosa ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Nucleotide sequence ; Peptide Synthases - genetics ; Peptide synthetase ; Peptides ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Toxins</subject><ispartof>FEMS microbiology letters, 1996-01, Vol.135 (2-3), p.295-303</ispartof><rights>1996 Federation of European Microbiological Societies. All rights reserved 1996</rights><rights>1996 INIST-CNRS</rights><rights>1996 Federation of European Microbiological Societies. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4805-b49f3503cd4c4443a31fd30249a9814c4c0df43d2e023624125394ca78bbbc2a3</citedby><cites>FETCH-LOGICAL-c4805-b49f3503cd4c4443a31fd30249a9814c4c0df43d2e023624125394ca78bbbc2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1996.tb08004.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1996.tb08004.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2975335$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8595871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meiβner, Kathrin</creatorcontrib><creatorcontrib>Dittmann, Elke</creatorcontrib><creatorcontrib>Börner, Thomas</creatorcontrib><title>Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Toxic strains of Microcystis aeruginosa produce cyclic heptatoxins (microcystins) that are believed to be synthesized non-ribosomally by peptide synthetases. We analysed toxin-producing and non-toxic strains of M. aeruginosa with respect to the presence of DNA sequences potentially encoding peptide synthetases. Hybridizations of genomic DNA of various M. aeruginosa strains with PCR-amplificated fragments possessing homologies to adenylate-forming domains of peptide synthetase genes provided first evidence for the existence of corresponding genes in cyanobacteria. Furthermore we isolated and sequenced from genomic libraries overlapping fragments of M. aeruginosa DNA with a total length of 2982 bp showing significant homology to genes encoding peptide synthetases and hybridizing exclusively with DNA from toxic strains. Our results indicate that both toxic and non-toxic strains of M. aeruginosa possess genes coding for peptide synthetases and that hepatotoxin-producing and non-toxic strains differ in their content of genes for specific peptide synthetases.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Toxins</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Conserved Sequence - genetics</subject><subject>Cyanobacteria</subject><subject>Cyanophyta</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Domains</subject><subject>Fragments</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetics</subject><subject>Hepatotoxicity</subject><subject>Homology</subject><subject>Microbiology</subject><subject>Microcysti</subject><subject>Microcystins</subject><subject>Microcystis</subject><subject>Microcystis - enzymology</subject><subject>Microcystis - genetics</subject><subject>Microcystis aeruginosa</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Nucleotide sequence</subject><subject>Peptide Synthases - genetics</subject><subject>Peptide synthetase</subject><subject>Peptides</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Toxins</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkc-L1DAcxYMo67j6JwhBZW-t-d3GgyDLrgqzeFnPIU3T2QxtMjYpTo_-56ZOmYOoYC4h-b73eMkHgFcYlTivt_sS84oVQoq6xFKKMjWoRoiVx0dgcx49BhtEq7rASFZPwbMY9yhrCBIX4KLmktcV3oAf9-HoDNS-hT74Iv06xTRq5yMMHUwPFppZ-9Bok-zopgHeOTMGM8fkItR2nHbOh6ihCT5lF4z222S9sRE-hCH0YRemCFOAB3tIrrUwzj6HJh0t3Flv43PwpNN9tC_W_RJ8vb25v_5UbL98_Hz9YVsYViNeNEx2lCNqWmYYY1RT3LUUESa1rHG-M6jtGG2JRYQKwjDhVDKjq7ppGkM0vQRXp9zDGHLDmNTgorF9r73NFRUWHEvCaBa-_k24D9PoczdFKMU1EoSSrHp3UuXPiHG0nTqMbtDjrDBSCyW1VwsKtaBQCyW1UlLHbGb_MCMs1EJOLeSU5CqbhFQi216uzaZmsO3ZtNLM8zfrXEej-27U3rh4lhFZcUp5lr0_yb673s7_0Vvd3m2JXAL4KSBMh7_Yiz89-ye0OtDY</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Meiβner, Kathrin</creator><creator>Dittmann, Elke</creator><creator>Börner, Thomas</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>199601</creationdate><title>Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes</title><author>Meiβner, Kathrin ; Dittmann, Elke ; Börner, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4805-b49f3503cd4c4443a31fd30249a9814c4c0df43d2e023624125394ca78bbbc2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Toxins</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Conserved Sequence - genetics</topic><topic>Cyanobacteria</topic><topic>Cyanophyta</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>Domains</topic><topic>Fragments</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetics</topic><topic>Hepatotoxicity</topic><topic>Homology</topic><topic>Microbiology</topic><topic>Microcysti</topic><topic>Microcystins</topic><topic>Microcystis</topic><topic>Microcystis - enzymology</topic><topic>Microcystis - genetics</topic><topic>Microcystis aeruginosa</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Nucleotide sequence</topic><topic>Peptide Synthases - genetics</topic><topic>Peptide synthetase</topic><topic>Peptides</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meiβner, Kathrin</creatorcontrib><creatorcontrib>Dittmann, Elke</creatorcontrib><creatorcontrib>Börner, Thomas</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meiβner, Kathrin</au><au>Dittmann, Elke</au><au>Börner, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1996-01</date><risdate>1996</risdate><volume>135</volume><issue>2-3</issue><spage>295</spage><epage>303</epage><pages>295-303</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Toxic strains of Microcystis aeruginosa produce cyclic heptatoxins (microcystins) that are believed to be synthesized non-ribosomally by peptide synthetases. We analysed toxin-producing and non-toxic strains of M. aeruginosa with respect to the presence of DNA sequences potentially encoding peptide synthetases. Hybridizations of genomic DNA of various M. aeruginosa strains with PCR-amplificated fragments possessing homologies to adenylate-forming domains of peptide synthetase genes provided first evidence for the existence of corresponding genes in cyanobacteria. Furthermore we isolated and sequenced from genomic libraries overlapping fragments of M. aeruginosa DNA with a total length of 2982 bp showing significant homology to genes encoding peptide synthetases and hybridizing exclusively with DNA from toxic strains. Our results indicate that both toxic and non-toxic strains of M. aeruginosa possess genes coding for peptide synthetases and that hepatotoxin-producing and non-toxic strains differ in their content of genes for specific peptide synthetases.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8595871</pmid><doi>10.1111/j.1574-6968.1996.tb08004.x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1996-01, Vol.135 (2-3), p.295-303 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16519243 |
| source | Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Toxins Bacteriology Base Sequence Biological and medical sciences Conserved Sequence - genetics Cyanobacteria Cyanophyta Deoxyribonucleic acid DNA DNA, Bacterial - analysis DNA, Bacterial - genetics Domains Fragments Freshwater Fundamental and applied biological sciences. Psychology Gene sequencing Genes Genes, Bacterial - genetics Genetics Hepatotoxicity Homology Microbiology Microcysti Microcystins Microcystis Microcystis - enzymology Microcystis - genetics Microcystis aeruginosa Molecular Sequence Data Nucleic Acid Hybridization Nucleotide sequence Peptide Synthases - genetics Peptide synthetase Peptides Sequence Analysis, DNA Sequence Homology, Nucleic Acid Toxins |
| title | Toxic and non-toxic strains of the cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes |
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