Enhanced extracellular production of recombinant Bacillus deramificans pullulanase in Escherichia coli through induction mode optimization and a glycine feeding strategy

•The pullulanase secretion was enhanced by optimized induction and glycine supplement.•The pullulanase activity represented the highest reported so far.•The approaches are valuable for extracellular secretion of other proteins in E. coli. Process optimization strategies were developed to improve ext...

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