3D label-free prostate specific antigen (PSA) immunosensor based on graphene–gold composites
Highly sensitive and label-free detection of the prostate specific antigen (PSA) remains a challenge in the diagnosis of prostate cancer. Here, a novel three-dimensional (3D) electrochemical immunosensor capable of sensitive and label-free detection of PSA is reported. This unique immunosensor is eq...
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| Veröffentlicht in: | Biosensors & bioelectronics 2015-01, Vol.63, p.546-551 |
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| creator | Jang, Hee Dong Kim, Sun Kyung Chang, Hankwon Choi, Jeong-Woo |
| description | Highly sensitive and label-free detection of the prostate specific antigen (PSA) remains a challenge in the diagnosis of prostate cancer. Here, a novel three-dimensional (3D) electrochemical immunosensor capable of sensitive and label-free detection of PSA is reported. This unique immunosensor is equipped with a highly conductive graphene (GR)-based gold (Au) composite modified electrode. The GR-based Au composite is prepared using aerosol spray pyrolysis and the morphology of the composite is the shape of a crumpled GR ball decorated with Au nanoparticles. Unlike the previous research, this novel 3D immunosensor functions very well over a broad linear range of 0–10ng/mL with a low detection limit of 0.59ng/mL; furthermore, it exhibits a significantly increased electron transfer and high sensitivity toward PSA. The highest rate of current change with respect to the PSA concentration is 5μA/(ng/mL). Satisfactory selectivity, reproducibility, and stability of the 3D immunosensor are also exhibited.
•A novel 3D label-free PSA immunosensor based on GR–Au composites was successfully developed for the detection of prostate cancer.•The GR-based Au composite is prepared using aerosol spray pyrolysis and the morphology of the composite is the shape of a crumpled GR ball decorated with Au nanoparticles.•The amperometric response of the 3D label-free PSA immunosensor exhibited stronger redox, higher current flow, and smaller electron transfer resistance compared with the 2D GR–Au modified electrode. |
| doi_str_mv | 10.1016/j.bios.2014.08.008 |
| format | Article |
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•A novel 3D label-free PSA immunosensor based on GR–Au composites was successfully developed for the detection of prostate cancer.•The GR-based Au composite is prepared using aerosol spray pyrolysis and the morphology of the composite is the shape of a crumpled GR ball decorated with Au nanoparticles.•The amperometric response of the 3D label-free PSA immunosensor exhibited stronger redox, higher current flow, and smaller electron transfer resistance compared with the 2D GR–Au modified electrode.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.08.008</identifier><identifier>PMID: 25150936</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Aerosol spray pyrolysis ; Antibodies - chemistry ; Antibodies - immunology ; Antigens ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensors ; Biotechnology ; Conductometry - instrumentation ; Crumpled graphene–gold composite ; Electrodes ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Gold ; Gold - chemistry ; Graphene ; Graphite - chemistry ; Immunoassay - instrumentation ; Immunosensors ; Label-free immunosensor ; Metal Nanoparticles - chemistry ; Methods. Procedures. Technologies ; Nanocomposites - chemistry ; Nanocomposites - ultrastructure ; Oxides - chemistry ; Prostate ; Prostate specific antigen (PSA) ; Prostate-Specific Antigen - analysis ; Prostate-Specific Antigen - chemistry ; Prostate-Specific Antigen - immunology ; Staining and Labeling ; Three dimensional ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2015-01, Vol.63, p.546-551</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-452cc5c7ab6627cb7145c2246eebca0d02004af614d285da94bdadef1affc3c23</citedby><cites>FETCH-LOGICAL-c452t-452cc5c7ab6627cb7145c2246eebca0d02004af614d285da94bdadef1affc3c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2014.08.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28831788$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25150936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jang, Hee Dong</creatorcontrib><creatorcontrib>Kim, Sun Kyung</creatorcontrib><creatorcontrib>Chang, Hankwon</creatorcontrib><creatorcontrib>Choi, Jeong-Woo</creatorcontrib><title>3D label-free prostate specific antigen (PSA) immunosensor based on graphene–gold composites</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Highly sensitive and label-free detection of the prostate specific antigen (PSA) remains a challenge in the diagnosis of prostate cancer. Here, a novel three-dimensional (3D) electrochemical immunosensor capable of sensitive and label-free detection of PSA is reported. This unique immunosensor is equipped with a highly conductive graphene (GR)-based gold (Au) composite modified electrode. The GR-based Au composite is prepared using aerosol spray pyrolysis and the morphology of the composite is the shape of a crumpled GR ball decorated with Au nanoparticles. Unlike the previous research, this novel 3D immunosensor functions very well over a broad linear range of 0–10ng/mL with a low detection limit of 0.59ng/mL; furthermore, it exhibits a significantly increased electron transfer and high sensitivity toward PSA. The highest rate of current change with respect to the PSA concentration is 5μA/(ng/mL). Satisfactory selectivity, reproducibility, and stability of the 3D immunosensor are also exhibited.
•A novel 3D label-free PSA immunosensor based on GR–Au composites was successfully developed for the detection of prostate cancer.•The GR-based Au composite is prepared using aerosol spray pyrolysis and the morphology of the composite is the shape of a crumpled GR ball decorated with Au nanoparticles.•The amperometric response of the 3D label-free PSA immunosensor exhibited stronger redox, higher current flow, and smaller electron transfer resistance compared with the 2D GR–Au modified electrode.</description><subject>Aerosol spray pyrolysis</subject><subject>Antibodies - chemistry</subject><subject>Antibodies - immunology</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Conductometry - instrumentation</subject><subject>Crumpled graphene–gold composite</subject><subject>Electrodes</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Graphene</subject><subject>Graphite - chemistry</subject><subject>Immunoassay - instrumentation</subject><subject>Immunosensors</subject><subject>Label-free immunosensor</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Nanocomposites - chemistry</subject><subject>Nanocomposites - ultrastructure</subject><subject>Oxides - chemistry</subject><subject>Prostate</subject><subject>Prostate specific antigen (PSA)</subject><subject>Prostate-Specific Antigen - analysis</subject><subject>Prostate-Specific Antigen - chemistry</subject><subject>Prostate-Specific Antigen - immunology</subject><subject>Staining and Labeling</subject><subject>Three dimensional</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2K1EAQxxtR3HH1BTxIX4T1kNjf6YCXZdcvWFBQrzad6srYQ5KO3RnBm-_gG_okZphRb-ql6vKrP1X1I-QhZzVn3Dzd1V1MpRaMq5rZmjF7i2y4bWSlhNS3yYa12lTaGHlG7pWyY4w1vGV3yZnQXLNWmg35KK_p4Dscqj4j0jmnsvgFaZkRYh-B-mmJW5zoxdt3l09oHMf9lApOJWXa-YKBpolus58_4YQ_vn3fpiFQSOOcSlyw3Cd3ej8UfHDq5-TDi-fvr15VN29evr66vKlAabFUawHQ0PjOGNFA13ClQQhlEDvwLDDBmPK94SoIq4NvVRd8wJ77vgcJQp6Ti2PuesDnPZbFjbEADoOfMO2L40ZzJayU5j9QqZloW8H_jWrdGm61VisqjiisHywZezfnOPr81XHmDrbczh1suYMtx6xbba1Dj075-27E8Hvkl54VeHwCfAE_9NlPEMsfzlrJG3sIenbkcP3xl4jZFYg4AYaYERYXUvzbHj8BM5uzmg</recordid><startdate>20150115</startdate><enddate>20150115</enddate><creator>Jang, Hee Dong</creator><creator>Kim, Sun Kyung</creator><creator>Chang, Hankwon</creator><creator>Choi, Jeong-Woo</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7SR</scope><scope>7U5</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150115</creationdate><title>3D label-free prostate specific antigen (PSA) immunosensor based on graphene–gold composites</title><author>Jang, Hee Dong ; Kim, Sun Kyung ; Chang, Hankwon ; Choi, Jeong-Woo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-452cc5c7ab6627cb7145c2246eebca0d02004af614d285da94bdadef1affc3c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aerosol spray pyrolysis</topic><topic>Antibodies - chemistry</topic><topic>Antibodies - immunology</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Conductometry - instrumentation</topic><topic>Crumpled graphene–gold composite</topic><topic>Electrodes</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Graphene</topic><topic>Graphite - chemistry</topic><topic>Immunoassay - instrumentation</topic><topic>Immunosensors</topic><topic>Label-free immunosensor</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Nanocomposites - chemistry</topic><topic>Nanocomposites - ultrastructure</topic><topic>Oxides - chemistry</topic><topic>Prostate</topic><topic>Prostate specific antigen (PSA)</topic><topic>Prostate-Specific Antigen - analysis</topic><topic>Prostate-Specific Antigen - chemistry</topic><topic>Prostate-Specific Antigen - immunology</topic><topic>Staining and Labeling</topic><topic>Three dimensional</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jang, Hee Dong</creatorcontrib><creatorcontrib>Kim, Sun Kyung</creatorcontrib><creatorcontrib>Chang, Hankwon</creatorcontrib><creatorcontrib>Choi, Jeong-Woo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jang, Hee Dong</au><au>Kim, Sun Kyung</au><au>Chang, Hankwon</au><au>Choi, Jeong-Woo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>3D label-free prostate specific antigen (PSA) immunosensor based on graphene–gold composites</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2015-01-15</date><risdate>2015</risdate><volume>63</volume><spage>546</spage><epage>551</epage><pages>546-551</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Highly sensitive and label-free detection of the prostate specific antigen (PSA) remains a challenge in the diagnosis of prostate cancer. Here, a novel three-dimensional (3D) electrochemical immunosensor capable of sensitive and label-free detection of PSA is reported. This unique immunosensor is equipped with a highly conductive graphene (GR)-based gold (Au) composite modified electrode. The GR-based Au composite is prepared using aerosol spray pyrolysis and the morphology of the composite is the shape of a crumpled GR ball decorated with Au nanoparticles. Unlike the previous research, this novel 3D immunosensor functions very well over a broad linear range of 0–10ng/mL with a low detection limit of 0.59ng/mL; furthermore, it exhibits a significantly increased electron transfer and high sensitivity toward PSA. The highest rate of current change with respect to the PSA concentration is 5μA/(ng/mL). Satisfactory selectivity, reproducibility, and stability of the 3D immunosensor are also exhibited.
•A novel 3D label-free PSA immunosensor based on GR–Au composites was successfully developed for the detection of prostate cancer.•The GR-based Au composite is prepared using aerosol spray pyrolysis and the morphology of the composite is the shape of a crumpled GR ball decorated with Au nanoparticles.•The amperometric response of the 3D label-free PSA immunosensor exhibited stronger redox, higher current flow, and smaller electron transfer resistance compared with the 2D GR–Au modified electrode.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>25150936</pmid><doi>10.1016/j.bios.2014.08.008</doi><tpages>6</tpages></addata></record> |
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| subjects | Aerosol spray pyrolysis Antibodies - chemistry Antibodies - immunology Antigens Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Conductometry - instrumentation Crumpled graphene–gold composite Electrodes Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Gold Gold - chemistry Graphene Graphite - chemistry Immunoassay - instrumentation Immunosensors Label-free immunosensor Metal Nanoparticles - chemistry Methods. Procedures. Technologies Nanocomposites - chemistry Nanocomposites - ultrastructure Oxides - chemistry Prostate Prostate specific antigen (PSA) Prostate-Specific Antigen - analysis Prostate-Specific Antigen - chemistry Prostate-Specific Antigen - immunology Staining and Labeling Three dimensional Various methods and equipments |
| title | 3D label-free prostate specific antigen (PSA) immunosensor based on graphene–gold composites |
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