Application of Gold Nanoparticles for Electrochemical DNA Biosensor
An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2 thin films. The SiO2 thin films surface was chemically modified with a mixture of APTES and GNPs fo...
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| Veröffentlicht in: | Journal of nanomaterials 2014-01, Vol.2014 (2014), p.1-7 |
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| container_title | Journal of nanomaterials |
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| creator | Al-Douri, Yarub Hisham, Hasrul Loong, Foo Kai Ibraheem, Ibraheem Jaleel Rahim, Ruslinda A. Mohammed, Ahmed Mishaal Hashim, Uda |
| description | An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2 thin films. The SiO2 thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2 thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2 thin films using APTES and GNPs were successfully developed for DNA detection. |
| doi_str_mv | 10.1155/2014/683460 |
| format | Article |
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The SiO2 thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2 thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2 thin films using APTES and GNPs were successfully developed for DNA detection.</description><identifier>ISSN: 1687-4110</identifier><identifier>EISSN: 1687-4129</identifier><identifier>DOI: 10.1155/2014/683460</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Biosensors ; Capacitance ; Chemistry ; Deoxyribonucleic acid ; DNA ; Electrodes ; Genetic diversity ; Gold ; Hybridization ; Immobilization ; Membrane filters ; Nanomaterials ; Nanoparticles ; Silicon dioxide ; Thin films</subject><ispartof>Journal of nanomaterials, 2014-01, Vol.2014 (2014), p.1-7</ispartof><rights>Copyright © 2014 Ahmed Mishaal Mohammed et al.</rights><rights>Copyright © 2014 Ahmed Mishaal Mohammed et al. Ahmed Mishaal Mohammed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a495t-447309299b3d131a742dd6dbbf45102ccd924fdbf3a7dd8a25b62988ad05c38d3</citedby><cites>FETCH-LOGICAL-a495t-447309299b3d131a742dd6dbbf45102ccd924fdbf3a7dd8a25b62988ad05c38d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><contributor>Ghoranneviss, M.</contributor><creatorcontrib>Al-Douri, Yarub</creatorcontrib><creatorcontrib>Hisham, Hasrul</creatorcontrib><creatorcontrib>Loong, Foo Kai</creatorcontrib><creatorcontrib>Ibraheem, Ibraheem Jaleel</creatorcontrib><creatorcontrib>Rahim, Ruslinda A.</creatorcontrib><creatorcontrib>Mohammed, Ahmed Mishaal</creatorcontrib><creatorcontrib>Hashim, Uda</creatorcontrib><title>Application of Gold Nanoparticles for Electrochemical DNA Biosensor</title><title>Journal of nanomaterials</title><description>An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2 thin films. The SiO2 thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2 thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2 thin films using APTES and GNPs were successfully developed for DNA detection.</description><subject>Biosensors</subject><subject>Capacitance</subject><subject>Chemistry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Electrodes</subject><subject>Genetic diversity</subject><subject>Gold</subject><subject>Hybridization</subject><subject>Immobilization</subject><subject>Membrane filters</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Silicon dioxide</subject><subject>Thin films</subject><issn>1687-4110</issn><issn>1687-4129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0M9LwzAUwPEiCs7pybsUvIhSl5dfTY5zzimMedFzSZOUdXRNTTrE_96MioiXnd47fHjwvklyCegegLEJRkAnXBDK0VEyAi7yjAKWx787oNPkLIQNQpRJhkfJbNp1Ta1VX7s2dVW6cI1JV6p1nfJ9rRsb0sr5dN5Y3Xun13YbcZM-rqbpQ-2CbYPz58lJpZpgL37mOHl_mr_NnrPl6-JlNl1mikrWZ5TmBEksZUkMEFA5xcZwU5YVZYCw1kZiWpmyIio3RijMSo6lEMogpokwZJzcDHc77z52NvTFtg7aNo1qrduFAjgDCjQX6DBlPGaSkuNIr__Rjdv5Nj4SFc0lcJ7TqO4Gpb0Lwduq6Hy9Vf6rAFTs2xf79sXQPurbQa_r1qjP-gC-GrCNxFbqD6YgECbfLgKK0g</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Al-Douri, Yarub</creator><creator>Hisham, Hasrul</creator><creator>Loong, Foo Kai</creator><creator>Ibraheem, Ibraheem Jaleel</creator><creator>Rahim, Ruslinda A.</creator><creator>Mohammed, Ahmed Mishaal</creator><creator>Hashim, Uda</creator><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QO</scope><scope>7TM</scope><scope>FR3</scope><scope>P64</scope><scope>F28</scope></search><sort><creationdate>20140101</creationdate><title>Application of Gold Nanoparticles for Electrochemical DNA Biosensor</title><author>Al-Douri, Yarub ; Hisham, Hasrul ; Loong, Foo Kai ; Ibraheem, Ibraheem Jaleel ; Rahim, Ruslinda A. ; Mohammed, Ahmed Mishaal ; Hashim, Uda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a495t-447309299b3d131a742dd6dbbf45102ccd924fdbf3a7dd8a25b62988ad05c38d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biosensors</topic><topic>Capacitance</topic><topic>Chemistry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Electrodes</topic><topic>Genetic diversity</topic><topic>Gold</topic><topic>Hybridization</topic><topic>Immobilization</topic><topic>Membrane filters</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Silicon dioxide</topic><topic>Thin films</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al-Douri, Yarub</creatorcontrib><creatorcontrib>Hisham, Hasrul</creatorcontrib><creatorcontrib>Loong, Foo Kai</creatorcontrib><creatorcontrib>Ibraheem, Ibraheem Jaleel</creatorcontrib><creatorcontrib>Rahim, Ruslinda A.</creatorcontrib><creatorcontrib>Mohammed, Ahmed Mishaal</creatorcontrib><creatorcontrib>Hashim, Uda</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><jtitle>Journal of nanomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Douri, Yarub</au><au>Hisham, Hasrul</au><au>Loong, Foo Kai</au><au>Ibraheem, Ibraheem Jaleel</au><au>Rahim, Ruslinda A.</au><au>Mohammed, Ahmed Mishaal</au><au>Hashim, Uda</au><au>Ghoranneviss, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Gold Nanoparticles for Electrochemical DNA Biosensor</atitle><jtitle>Journal of nanomaterials</jtitle><date>2014-01-01</date><risdate>2014</risdate><volume>2014</volume><issue>2014</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1687-4110</issn><eissn>1687-4129</eissn><abstract>An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2 thin films. The SiO2 thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2 thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2 thin films using APTES and GNPs were successfully developed for DNA detection.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><doi>10.1155/2014/683460</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biosensors Capacitance Chemistry Deoxyribonucleic acid DNA Electrodes Genetic diversity Gold Hybridization Immobilization Membrane filters Nanomaterials Nanoparticles Silicon dioxide Thin films |
| title | Application of Gold Nanoparticles for Electrochemical DNA Biosensor |
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