An integrated multifunctional platform based on biotin-doped conducting polymer nanowires for cell capture, release, and electrochemical sensing
Abstract Here, we propose an integrated multifunctional system constructed by conductive disulfide-biotin-doped polypyrrole nanowires (SS-biotin-Ppy NWs) for capture, release, and in situ quantification of circulating tumor cells (CTCs). A well-ordered three-dimensional nanowire structure equipped w...
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| Veröffentlicht in: | Biomaterials 2014-12, Vol.35 (36), p.9573-9580 |
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| creator | Hong, Woo Young Jeon, Seung Hyun Lee, Eun Sook Cho, Youngnam |
| description | Abstract Here, we propose an integrated multifunctional system constructed by conductive disulfide-biotin-doped polypyrrole nanowires (SS-biotin-Ppy NWs) for capture, release, and in situ quantification of circulating tumor cells (CTCs). A well-ordered three-dimensional nanowire structure equipped with a monoclonal antibody offers a significant impact on the cell-capture efficiency, as well as on electrical- or glutathione (GSH)-mediated release of the captured cells. In addition, the electrochemical identification/detection of the captured cancer cells can be directly conducted on the same Ppy NW platform by using horseradish peroxidase (HRP)-labeled and anti-EpCAM-conjugated nanoparticles (HRP/anti-EpCAM Ppy NPs), showing very high sensitivity and specificity. The signal amplification can be clearly attributed to the catalytic response resulting from enzymatic reduction of hydrogen peroxide on Ppy NWs, consequently generating a greatly increased amperometric response with a detection range of 10 to 1 × 104 cells and a detection limit of as low as 10 cells. Overall, the proposed Ppy NWs not only present a promising platform for effective cell capture and release but also permit cytosensing capability for on-site analysis. |
| doi_str_mv | 10.1016/j.biomaterials.2014.08.027 |
| format | Article |
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A well-ordered three-dimensional nanowire structure equipped with a monoclonal antibody offers a significant impact on the cell-capture efficiency, as well as on electrical- or glutathione (GSH)-mediated release of the captured cells. In addition, the electrochemical identification/detection of the captured cancer cells can be directly conducted on the same Ppy NW platform by using horseradish peroxidase (HRP)-labeled and anti-EpCAM-conjugated nanoparticles (HRP/anti-EpCAM Ppy NPs), showing very high sensitivity and specificity. The signal amplification can be clearly attributed to the catalytic response resulting from enzymatic reduction of hydrogen peroxide on Ppy NWs, consequently generating a greatly increased amperometric response with a detection range of 10 to 1 × 104 cells and a detection limit of as low as 10 cells. Overall, the proposed Ppy NWs not only present a promising platform for effective cell capture and release but also permit cytosensing capability for on-site analysis.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2014.08.027</identifier><identifier>PMID: 25192586</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Amplification ; Antibodies, Immobilized - chemistry ; Biosensing Techniques - instrumentation ; Biotin - chemistry ; Cancer diagnosis ; Cell Line, Tumor ; Cell Separation - instrumentation ; Circulating ; Circulating tumor cells ; Conducting polymer ; Dentistry ; Electrical stimulation ; Electrochemical Techniques - instrumentation ; Equipment Design ; Humans ; Immunohistochemistry - instrumentation ; Immunosensor ; Limit of Detection ; Nanowires ; Nanowires - chemistry ; Nanowires - ultrastructure ; Neoplasms - diagnosis ; Platforms ; Polymers - chemistry ; Polypyrroles ; Pyrroles - chemistry ; Reduction ; Surgical implants ; Three dimensional</subject><ispartof>Biomaterials, 2014-12, Vol.35 (36), p.9573-9580</ispartof><rights>Elsevier Ltd</rights><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c637t-31b27d7a6e0a221582e65bf0f171ae5f9caa6f49e516ed162f3a496fb0295be43</citedby><cites>FETCH-LOGICAL-c637t-31b27d7a6e0a221582e65bf0f171ae5f9caa6f49e516ed162f3a496fb0295be43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961214009521$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25192586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hong, Woo Young</creatorcontrib><creatorcontrib>Jeon, Seung Hyun</creatorcontrib><creatorcontrib>Lee, Eun Sook</creatorcontrib><creatorcontrib>Cho, Youngnam</creatorcontrib><title>An integrated multifunctional platform based on biotin-doped conducting polymer nanowires for cell capture, release, and electrochemical sensing</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Here, we propose an integrated multifunctional system constructed by conductive disulfide-biotin-doped polypyrrole nanowires (SS-biotin-Ppy NWs) for capture, release, and in situ quantification of circulating tumor cells (CTCs). A well-ordered three-dimensional nanowire structure equipped with a monoclonal antibody offers a significant impact on the cell-capture efficiency, as well as on electrical- or glutathione (GSH)-mediated release of the captured cells. In addition, the electrochemical identification/detection of the captured cancer cells can be directly conducted on the same Ppy NW platform by using horseradish peroxidase (HRP)-labeled and anti-EpCAM-conjugated nanoparticles (HRP/anti-EpCAM Ppy NPs), showing very high sensitivity and specificity. The signal amplification can be clearly attributed to the catalytic response resulting from enzymatic reduction of hydrogen peroxide on Ppy NWs, consequently generating a greatly increased amperometric response with a detection range of 10 to 1 × 104 cells and a detection limit of as low as 10 cells. Overall, the proposed Ppy NWs not only present a promising platform for effective cell capture and release but also permit cytosensing capability for on-site analysis.</description><subject>Advanced Basic Science</subject><subject>Amplification</subject><subject>Antibodies, Immobilized - chemistry</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotin - chemistry</subject><subject>Cancer diagnosis</subject><subject>Cell Line, Tumor</subject><subject>Cell Separation - instrumentation</subject><subject>Circulating</subject><subject>Circulating tumor cells</subject><subject>Conducting polymer</subject><subject>Dentistry</subject><subject>Electrical stimulation</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Equipment Design</subject><subject>Humans</subject><subject>Immunohistochemistry - instrumentation</subject><subject>Immunosensor</subject><subject>Limit of Detection</subject><subject>Nanowires</subject><subject>Nanowires - chemistry</subject><subject>Nanowires - ultrastructure</subject><subject>Neoplasms - diagnosis</subject><subject>Platforms</subject><subject>Polymers - chemistry</subject><subject>Polypyrroles</subject><subject>Pyrroles - chemistry</subject><subject>Reduction</subject><subject>Surgical implants</subject><subject>Three dimensional</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUstu1TAQjRCIXgq_gCxWLJrgcWInZoFUtbykSiyAteU4k-JLYgfbKbp_wSfX0S0IsaErezTnMfaZongBtAIK4tW-6q2fdcJg9RQrRqGpaFdR1j4odtC1Xckl5Q-LXW6wUgpgJ8WTGPc017Rhj4sTxkEy3old8evcEesSXoesN5B5nZIdV2eS9U5PZJl0Gn2YSa9jbntHsnWyrhz8kmvj3bBmrLsmi58OMwbitPM_bcBIMo8YnCZi9JLWgGck4IRZ54xoN5B8Nyl48w1na7JVRBez0NPi0Zhfhc_uztPi67u3Xy4-lFef3n-8OL8qjajbVNbQs3ZotUCqGQPeMRS8H-kILWjkozRai7GRyEHgAIKNtW6kGHvKJO-xqU-Ll0fdJfgfK8akZhu3cbVDv0YFgkMDwCS7B7SWUjJJxf-hXLCmhlbIDH19hJrgYww4qiXYWYeDAqq2nNVe_Z2z2nJWtFM550x-fuez9jMOf6i_g82AyyMA8x_eWAwqGovO4JCzMUkN3t7P580_MmaybsvrOx4w7v0a3MYBFZmi6vO2cdvC5UWjkjOobwEAN9gA</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Hong, Woo Young</creator><creator>Jeon, Seung Hyun</creator><creator>Lee, Eun Sook</creator><creator>Cho, Youngnam</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20141201</creationdate><title>An integrated multifunctional platform based on biotin-doped conducting polymer nanowires for cell capture, release, and electrochemical sensing</title><author>Hong, Woo Young ; Jeon, Seung Hyun ; Lee, Eun Sook ; Cho, Youngnam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c637t-31b27d7a6e0a221582e65bf0f171ae5f9caa6f49e516ed162f3a496fb0295be43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Advanced Basic Science</topic><topic>Amplification</topic><topic>Antibodies, Immobilized - chemistry</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotin - chemistry</topic><topic>Cancer diagnosis</topic><topic>Cell Line, Tumor</topic><topic>Cell Separation - instrumentation</topic><topic>Circulating</topic><topic>Circulating tumor cells</topic><topic>Conducting polymer</topic><topic>Dentistry</topic><topic>Electrical stimulation</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Equipment Design</topic><topic>Humans</topic><topic>Immunohistochemistry - instrumentation</topic><topic>Immunosensor</topic><topic>Limit of Detection</topic><topic>Nanowires</topic><topic>Nanowires - chemistry</topic><topic>Nanowires - ultrastructure</topic><topic>Neoplasms - diagnosis</topic><topic>Platforms</topic><topic>Polymers - chemistry</topic><topic>Polypyrroles</topic><topic>Pyrroles - chemistry</topic><topic>Reduction</topic><topic>Surgical implants</topic><topic>Three dimensional</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hong, Woo Young</creatorcontrib><creatorcontrib>Jeon, Seung Hyun</creatorcontrib><creatorcontrib>Lee, Eun Sook</creatorcontrib><creatorcontrib>Cho, Youngnam</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hong, Woo Young</au><au>Jeon, Seung Hyun</au><au>Lee, Eun Sook</au><au>Cho, Youngnam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An integrated multifunctional platform based on biotin-doped conducting polymer nanowires for cell capture, release, and electrochemical sensing</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>35</volume><issue>36</issue><spage>9573</spage><epage>9580</epage><pages>9573-9580</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Here, we propose an integrated multifunctional system constructed by conductive disulfide-biotin-doped polypyrrole nanowires (SS-biotin-Ppy NWs) for capture, release, and in situ quantification of circulating tumor cells (CTCs). A well-ordered three-dimensional nanowire structure equipped with a monoclonal antibody offers a significant impact on the cell-capture efficiency, as well as on electrical- or glutathione (GSH)-mediated release of the captured cells. In addition, the electrochemical identification/detection of the captured cancer cells can be directly conducted on the same Ppy NW platform by using horseradish peroxidase (HRP)-labeled and anti-EpCAM-conjugated nanoparticles (HRP/anti-EpCAM Ppy NPs), showing very high sensitivity and specificity. The signal amplification can be clearly attributed to the catalytic response resulting from enzymatic reduction of hydrogen peroxide on Ppy NWs, consequently generating a greatly increased amperometric response with a detection range of 10 to 1 × 104 cells and a detection limit of as low as 10 cells. 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| subjects | Advanced Basic Science Amplification Antibodies, Immobilized - chemistry Biosensing Techniques - instrumentation Biotin - chemistry Cancer diagnosis Cell Line, Tumor Cell Separation - instrumentation Circulating Circulating tumor cells Conducting polymer Dentistry Electrical stimulation Electrochemical Techniques - instrumentation Equipment Design Humans Immunohistochemistry - instrumentation Immunosensor Limit of Detection Nanowires Nanowires - chemistry Nanowires - ultrastructure Neoplasms - diagnosis Platforms Polymers - chemistry Polypyrroles Pyrroles - chemistry Reduction Surgical implants Three dimensional |
| title | An integrated multifunctional platform based on biotin-doped conducting polymer nanowires for cell capture, release, and electrochemical sensing |
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