Influence of in vitro assay pH and extractant composition on As bioaccessibility in contaminated soils
In vitro bioaccessibility assays are often utilised to determine the potential human exposure to soil contaminants through soil ingestion. Comparative studies have identified inconsistencies in the results obtained with different in vitro assays. In this study we investigated the potential causes fo...
Gespeichert in:
| Veröffentlicht in: | The Science of the total environment 2014-03, Vol.473-474, p.171-177 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 177 |
|---|---|
| container_issue | |
| container_start_page | 171 |
| container_title | The Science of the total environment |
| container_volume | 473-474 |
| creator | Smith, Euan Scheckel, Kirk Miller, Bradley W. Weber, John Juhasz, Albert L. |
| description | In vitro bioaccessibility assays are often utilised to determine the potential human exposure to soil contaminants through soil ingestion. Comparative studies have identified inconsistencies in the results obtained with different in vitro assays. In this study we investigated the potential causes for the variability between in vitro assay results using the PBET and SBRC assays to assess As bioaccessibility in 5 brownfield contaminated soils. Total As concentration in the 5 soils ranged from 227 to 807mg As kg−1 in the 88% was sorbed to Fe mineral phases) with the remaining As present as beudantite or orpiment mineral phases. Arsenic bioaccessibility varied depending on the in vitro methodology; markedly higher values were obtained using the SBRC gastric phase compared to the PBET gastric phase, however, similar As bioaccessibility values were obtained in both the SBRC and PBET intestinal phases. The difference in As bioaccessibility following SBRC and PBET gastric phase extraction appeared to be due to the difference in gastric phase pH (i.e. 1.5 versus 2.5 respectively), however, modifying the PBET gastric phase to pH1.5 (that of the SBRC gastric phase) enhanced As bioaccessibility up to 1.6 fold, but was still markedly lower than SBRC values. Although As bioaccessibility was enhanced, the increase did not occur as a result of the solubilisation of As associated Fe mineral phases suggesting As bioaccessibility may also be strongly influenced by the in vitro assay extractant composition. The extractant composition of the PBET assay incorporates a number of organic acids in addition to pepsin which may inhibit the sorption of As onto iron oxide surfaces, therefore increasing As solubility at the modified (pH1.5) gastric phase pH.
•In vitro assays are often used to estimate potential arsenic bioaccessibility.•The SBRC or PBET in vitro assays have been used to assess arsenic bioaccessibility.•Arsenic bioaccessibility was higher in the SBRC gastric phase.•Arsenic bioaccessibility was influenced by in vitro assay pH.•Solution composition of gastric phase influenced arsenic bioaccessibility. |
| doi_str_mv | 10.1016/j.scitotenv.2013.12.030 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1651407046</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0048969713014848</els_id><sourcerecordid>1651407046</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-e55e6faf3f543edaf96f4eb6e40cf0a284e8a833125d30772aa878801e053d6c3</originalsourceid><addsrcrecordid>eNqNkU1r3DAQhkVoSLZp_0KrYy92Rx-W7OMSmiYQ6KU5C608Ai22tJW0S_ff12bTXNthYC7PvAPzEPKZQcuAqa_7trhQU8V4ajkw0TLegoArsmG9HhoGXL0jGwDZN4Ma9C15X8oeltI9uyG3XAo18KHbEP8U_XTE6JAmT0Okp1BzorYUe6aHR2rjSPF3zdZVGyt1aT6kEmpIkS69LXQXknUOSwm7MIV6XjNcitXOIdqKIy0pTOUDufZ2Kvjxdd6Rl4dvP-8fm-cf35_ut8-N60DUBrsOlbde-E4KHK0flJe4UyjBebC8l9jbXgjGu1GA1tzaXvc9MIROjMqJO_LlknvI6dcRSzVzKA6nyUZMx2KY6pgEDVL9Byo1MD0otqD6grqcSsnozSGH2eazYWBWH2Zv3nyY1Ydh3Cw-ls1Pr0eOuxnHt72_AhZgewFw-copYF6DVh1jyOiqGVP455E_By2hvw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1647017961</pqid></control><display><type>article</type><title>Influence of in vitro assay pH and extractant composition on As bioaccessibility in contaminated soils</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Smith, Euan ; Scheckel, Kirk ; Miller, Bradley W. ; Weber, John ; Juhasz, Albert L.</creator><creatorcontrib>Smith, Euan ; Scheckel, Kirk ; Miller, Bradley W. ; Weber, John ; Juhasz, Albert L.</creatorcontrib><description>In vitro bioaccessibility assays are often utilised to determine the potential human exposure to soil contaminants through soil ingestion. Comparative studies have identified inconsistencies in the results obtained with different in vitro assays. In this study we investigated the potential causes for the variability between in vitro assay results using the PBET and SBRC assays to assess As bioaccessibility in 5 brownfield contaminated soils. Total As concentration in the 5 soils ranged from 227 to 807mg As kg−1 in the <250μm particle fraction while XANES analysis identified that As was predominately present as AsV (>88% was sorbed to Fe mineral phases) with the remaining As present as beudantite or orpiment mineral phases. Arsenic bioaccessibility varied depending on the in vitro methodology; markedly higher values were obtained using the SBRC gastric phase compared to the PBET gastric phase, however, similar As bioaccessibility values were obtained in both the SBRC and PBET intestinal phases. The difference in As bioaccessibility following SBRC and PBET gastric phase extraction appeared to be due to the difference in gastric phase pH (i.e. 1.5 versus 2.5 respectively), however, modifying the PBET gastric phase to pH1.5 (that of the SBRC gastric phase) enhanced As bioaccessibility up to 1.6 fold, but was still markedly lower than SBRC values. Although As bioaccessibility was enhanced, the increase did not occur as a result of the solubilisation of As associated Fe mineral phases suggesting As bioaccessibility may also be strongly influenced by the in vitro assay extractant composition. The extractant composition of the PBET assay incorporates a number of organic acids in addition to pepsin which may inhibit the sorption of As onto iron oxide surfaces, therefore increasing As solubility at the modified (pH1.5) gastric phase pH.
•In vitro assays are often used to estimate potential arsenic bioaccessibility.•The SBRC or PBET in vitro assays have been used to assess arsenic bioaccessibility.•Arsenic bioaccessibility was higher in the SBRC gastric phase.•Arsenic bioaccessibility was influenced by in vitro assay pH.•Solution composition of gastric phase influenced arsenic bioaccessibility.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2013.12.030</identifier><identifier>PMID: 24369295</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Arsenic ; Arsenic - analysis ; Assaying ; Bioaccessibility ; Biological Assay ; Contamination ; Environmental Monitoring - methods ; Exposure ; Hydrogen-Ion Concentration ; In vitro testing ; Iron ; Minerals ; Phases ; Soil - chemistry ; Soil Pollutants - analysis ; Soils ; Speciation ; XANES</subject><ispartof>The Science of the total environment, 2014-03, Vol.473-474, p.171-177</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-e55e6faf3f543edaf96f4eb6e40cf0a284e8a833125d30772aa878801e053d6c3</citedby><cites>FETCH-LOGICAL-c503t-e55e6faf3f543edaf96f4eb6e40cf0a284e8a833125d30772aa878801e053d6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0048969713014848$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24369295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Euan</creatorcontrib><creatorcontrib>Scheckel, Kirk</creatorcontrib><creatorcontrib>Miller, Bradley W.</creatorcontrib><creatorcontrib>Weber, John</creatorcontrib><creatorcontrib>Juhasz, Albert L.</creatorcontrib><title>Influence of in vitro assay pH and extractant composition on As bioaccessibility in contaminated soils</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>In vitro bioaccessibility assays are often utilised to determine the potential human exposure to soil contaminants through soil ingestion. Comparative studies have identified inconsistencies in the results obtained with different in vitro assays. In this study we investigated the potential causes for the variability between in vitro assay results using the PBET and SBRC assays to assess As bioaccessibility in 5 brownfield contaminated soils. Total As concentration in the 5 soils ranged from 227 to 807mg As kg−1 in the <250μm particle fraction while XANES analysis identified that As was predominately present as AsV (>88% was sorbed to Fe mineral phases) with the remaining As present as beudantite or orpiment mineral phases. Arsenic bioaccessibility varied depending on the in vitro methodology; markedly higher values were obtained using the SBRC gastric phase compared to the PBET gastric phase, however, similar As bioaccessibility values were obtained in both the SBRC and PBET intestinal phases. The difference in As bioaccessibility following SBRC and PBET gastric phase extraction appeared to be due to the difference in gastric phase pH (i.e. 1.5 versus 2.5 respectively), however, modifying the PBET gastric phase to pH1.5 (that of the SBRC gastric phase) enhanced As bioaccessibility up to 1.6 fold, but was still markedly lower than SBRC values. Although As bioaccessibility was enhanced, the increase did not occur as a result of the solubilisation of As associated Fe mineral phases suggesting As bioaccessibility may also be strongly influenced by the in vitro assay extractant composition. The extractant composition of the PBET assay incorporates a number of organic acids in addition to pepsin which may inhibit the sorption of As onto iron oxide surfaces, therefore increasing As solubility at the modified (pH1.5) gastric phase pH.
•In vitro assays are often used to estimate potential arsenic bioaccessibility.•The SBRC or PBET in vitro assays have been used to assess arsenic bioaccessibility.•Arsenic bioaccessibility was higher in the SBRC gastric phase.•Arsenic bioaccessibility was influenced by in vitro assay pH.•Solution composition of gastric phase influenced arsenic bioaccessibility.</description><subject>Arsenic</subject><subject>Arsenic - analysis</subject><subject>Assaying</subject><subject>Bioaccessibility</subject><subject>Biological Assay</subject><subject>Contamination</subject><subject>Environmental Monitoring - methods</subject><subject>Exposure</subject><subject>Hydrogen-Ion Concentration</subject><subject>In vitro testing</subject><subject>Iron</subject><subject>Minerals</subject><subject>Phases</subject><subject>Soil - chemistry</subject><subject>Soil Pollutants - analysis</subject><subject>Soils</subject><subject>Speciation</subject><subject>XANES</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1r3DAQhkVoSLZp_0KrYy92Rx-W7OMSmiYQ6KU5C608Ai22tJW0S_ff12bTXNthYC7PvAPzEPKZQcuAqa_7trhQU8V4ajkw0TLegoArsmG9HhoGXL0jGwDZN4Ma9C15X8oeltI9uyG3XAo18KHbEP8U_XTE6JAmT0Okp1BzorYUe6aHR2rjSPF3zdZVGyt1aT6kEmpIkS69LXQXknUOSwm7MIV6XjNcitXOIdqKIy0pTOUDufZ2Kvjxdd6Rl4dvP-8fm-cf35_ut8-N60DUBrsOlbde-E4KHK0flJe4UyjBebC8l9jbXgjGu1GA1tzaXvc9MIROjMqJO_LlknvI6dcRSzVzKA6nyUZMx2KY6pgEDVL9Byo1MD0otqD6grqcSsnozSGH2eazYWBWH2Zv3nyY1Ydh3Cw-ls1Pr0eOuxnHt72_AhZgewFw-copYF6DVh1jyOiqGVP455E_By2hvw</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Smith, Euan</creator><creator>Scheckel, Kirk</creator><creator>Miller, Bradley W.</creator><creator>Weber, John</creator><creator>Juhasz, Albert L.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U6</scope><scope>C1K</scope><scope>SOI</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20140301</creationdate><title>Influence of in vitro assay pH and extractant composition on As bioaccessibility in contaminated soils</title><author>Smith, Euan ; Scheckel, Kirk ; Miller, Bradley W. ; Weber, John ; Juhasz, Albert L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-e55e6faf3f543edaf96f4eb6e40cf0a284e8a833125d30772aa878801e053d6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Arsenic</topic><topic>Arsenic - analysis</topic><topic>Assaying</topic><topic>Bioaccessibility</topic><topic>Biological Assay</topic><topic>Contamination</topic><topic>Environmental Monitoring - methods</topic><topic>Exposure</topic><topic>Hydrogen-Ion Concentration</topic><topic>In vitro testing</topic><topic>Iron</topic><topic>Minerals</topic><topic>Phases</topic><topic>Soil - chemistry</topic><topic>Soil Pollutants - analysis</topic><topic>Soils</topic><topic>Speciation</topic><topic>XANES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Euan</creatorcontrib><creatorcontrib>Scheckel, Kirk</creatorcontrib><creatorcontrib>Miller, Bradley W.</creatorcontrib><creatorcontrib>Weber, John</creatorcontrib><creatorcontrib>Juhasz, Albert L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Euan</au><au>Scheckel, Kirk</au><au>Miller, Bradley W.</au><au>Weber, John</au><au>Juhasz, Albert L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of in vitro assay pH and extractant composition on As bioaccessibility in contaminated soils</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>473-474</volume><spage>171</spage><epage>177</epage><pages>171-177</pages><issn>0048-9697</issn><eissn>1879-1026</eissn><abstract>In vitro bioaccessibility assays are often utilised to determine the potential human exposure to soil contaminants through soil ingestion. Comparative studies have identified inconsistencies in the results obtained with different in vitro assays. In this study we investigated the potential causes for the variability between in vitro assay results using the PBET and SBRC assays to assess As bioaccessibility in 5 brownfield contaminated soils. Total As concentration in the 5 soils ranged from 227 to 807mg As kg−1 in the <250μm particle fraction while XANES analysis identified that As was predominately present as AsV (>88% was sorbed to Fe mineral phases) with the remaining As present as beudantite or orpiment mineral phases. Arsenic bioaccessibility varied depending on the in vitro methodology; markedly higher values were obtained using the SBRC gastric phase compared to the PBET gastric phase, however, similar As bioaccessibility values were obtained in both the SBRC and PBET intestinal phases. The difference in As bioaccessibility following SBRC and PBET gastric phase extraction appeared to be due to the difference in gastric phase pH (i.e. 1.5 versus 2.5 respectively), however, modifying the PBET gastric phase to pH1.5 (that of the SBRC gastric phase) enhanced As bioaccessibility up to 1.6 fold, but was still markedly lower than SBRC values. Although As bioaccessibility was enhanced, the increase did not occur as a result of the solubilisation of As associated Fe mineral phases suggesting As bioaccessibility may also be strongly influenced by the in vitro assay extractant composition. The extractant composition of the PBET assay incorporates a number of organic acids in addition to pepsin which may inhibit the sorption of As onto iron oxide surfaces, therefore increasing As solubility at the modified (pH1.5) gastric phase pH.
•In vitro assays are often used to estimate potential arsenic bioaccessibility.•The SBRC or PBET in vitro assays have been used to assess arsenic bioaccessibility.•Arsenic bioaccessibility was higher in the SBRC gastric phase.•Arsenic bioaccessibility was influenced by in vitro assay pH.•Solution composition of gastric phase influenced arsenic bioaccessibility.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24369295</pmid><doi>10.1016/j.scitotenv.2013.12.030</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0048-9697 |
| ispartof | The Science of the total environment, 2014-03, Vol.473-474, p.171-177 |
| issn | 0048-9697 1879-1026 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1651407046 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Arsenic Arsenic - analysis Assaying Bioaccessibility Biological Assay Contamination Environmental Monitoring - methods Exposure Hydrogen-Ion Concentration In vitro testing Iron Minerals Phases Soil - chemistry Soil Pollutants - analysis Soils Speciation XANES |
| title | Influence of in vitro assay pH and extractant composition on As bioaccessibility in contaminated soils |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T22%3A22%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20in%20vitro%20assay%20pH%20and%20extractant%20composition%20on%20As%20bioaccessibility%20in%20contaminated%20soils&rft.jtitle=The%20Science%20of%20the%20total%20environment&rft.au=Smith,%20Euan&rft.date=2014-03-01&rft.volume=473-474&rft.spage=171&rft.epage=177&rft.pages=171-177&rft.issn=0048-9697&rft.eissn=1879-1026&rft_id=info:doi/10.1016/j.scitotenv.2013.12.030&rft_dat=%3Cproquest_cross%3E1651407046%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1647017961&rft_id=info:pmid/24369295&rft_els_id=S0048969713014848&rfr_iscdi=true |