High-level expression and characterization of carboxypeptidase Y from Saccharomyces cerevisiae in Pichia pastoris GS115

Carboxypeptidase Y is widely used in peptide sequencing and mass spectrometry. PRC1 coding for proteinase C from Saccharomyces cerevisiae was expressed in Pichia pastoris GS115 as procarboxypeptidase Y with a yield of ~605 mg/l in shake-flasks after 168 h induction with 1 % (v/v) methanol. This prec...

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Veröffentlicht in:	Biotechnology letters 2015-01, Vol.37 (1), p.161-167
Hauptverfasser:	Yu, Xianhong, Zhai, Chao, Zhong, Xing, Tang, Wei, Wang, Xiaojuan, Yang, Hu, Chen, Wanping, Ma, Lixin
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Broths carboxypeptidase C Cathepsin A - chemistry Cathepsin A - genetics Cathepsin A - isolation & purification Cathepsin A - metabolism culture media Enzyme Stability Enzymes Fermentation Gene expression Life Sciences Mass spectrometry methanol Methyl alcohol Microbiology Optimization Original Research Paper Pichia - genetics Pichia - metabolism Pichia pastoris Proteinase Recombinant Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Yeast
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