Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry
•A method to quantify firocoxib by high-resolution orbitrap mass spectrometry.•Method validated with excellent recovery, linearity, precision and accuracy.•Method was successfully utilized for firocoxib pharmacokinetic analysis in camels.•The method was used to tentatively identify firocoxib metabol...
Gespeichert in:
| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2015-01, Vol.974, p.17-23 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 23 |
|---|---|
| container_issue | |
| container_start_page | 17 |
| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| container_volume | 974 |
| creator | Wasfi, Ibrahim A. Saeed, Hanan M. Agha, B.A. Kamel, Asmaa M. Biriki, Nasreen A. Al Neaimi, Khaled M. Al Ali, Waleed A. Al Sultan, Saeed Mahmoud |
| description | •A method to quantify firocoxib by high-resolution orbitrap mass spectrometry.•Method validated with excellent recovery, linearity, precision and accuracy.•Method was successfully utilized for firocoxib pharmacokinetic analysis in camels.•The method was used to tentatively identify firocoxib metabolite in camel plasma.
In this study, we developed a high-resolution liquid chromatography mass spectrometry method for the pharmacokinetic study of firocoxib followed by full method validation. Following a solid-phase extraction, the firocoxib and internal standard (celecoxib) were separated on an Agilent Zorbax ZDB C18 column (50mm×2.1mm i.d., 3.5μm) with a gradient elution using methanol and 0.1% aqueous formic acid. Data acquisition was performed at 25,000 resolution with the automatic gain set to 1,000,000 and the maximum injection time of 100ms. Data were acquired in full-scan mode over a mass range of 100–550Da in positive electrospray mode. Linear calibration curves were obtained over the concentration ranges of 0.5–200ng/mL and no interfering peaks were detected at the retention time of firocoxib and internal standard in blank camel plasma samples. The mean extraction recoveries of firocoxib at three concentrations of 5, 25 and 75ng/mL ranged from 92 to 104%. Coefficient of variation of intra-day and inter-day precision were both |
| doi_str_mv | 10.1016/j.jchromb.2014.10.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1651379887</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S157002321400662X</els_id><sourcerecordid>1639498305</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-aad0079b8aa98e53595246e6fac314d517847de6f13b2aedda39a7f94362992f3</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS0EoqXwCCAv2WTwTxLHK4QqfipVggVI7CzHvmk8xHawnYp5Fl4Wj2botiv7Hn_XV_cchF5TsqOE9u_2u72ZU_TjjhHaVm1HGH2CLukgeMNF__NpvXeCNIRxdoFe5LwnhAoi-HN0wbq251TyS_T326yT1yb-cgGKMxnrYLGHose4uOxxLps94DjhyaVo4h83Yhew0R6Wyk4FUq1L0vcQ4lYV611wuQrFxYDHA96yC3d4dndzkyDHZTs9QDBzU-KKYxpdxVfsdc44r2BKXQtKOrxEzya9ZHh1Pq_Qj08fv19_aW6_fr65_nDbGC6H0mhtCRFyHLSWA3S8kx1re-gnbThtbUfF0Apba8pHpsFazaUWk2x5z6RkE79Cb0__rin-3iAX5V02sCw6QN1J0b6jXMihOvs4ymUrB066inYn1KSYc4JJrcl5nQ6KEnWMUO3VOUJ1jPAo1whr35vziG30YB-6_mdWgfcnoCYA9w6SysZVO8G6VM1TNrpHRvwDWc20ag</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1639498305</pqid></control><display><type>article</type><title>Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Wasfi, Ibrahim A. ; Saeed, Hanan M. ; Agha, B.A. ; Kamel, Asmaa M. ; Biriki, Nasreen A. Al ; Neaimi, Khaled M. Al ; Ali, Waleed A. Al ; Sultan, Saeed Mahmoud</creator><creatorcontrib>Wasfi, Ibrahim A. ; Saeed, Hanan M. ; Agha, B.A. ; Kamel, Asmaa M. ; Biriki, Nasreen A. Al ; Neaimi, Khaled M. Al ; Ali, Waleed A. Al ; Sultan, Saeed Mahmoud</creatorcontrib><description>•A method to quantify firocoxib by high-resolution orbitrap mass spectrometry.•Method validated with excellent recovery, linearity, precision and accuracy.•Method was successfully utilized for firocoxib pharmacokinetic analysis in camels.•The method was used to tentatively identify firocoxib metabolite in camel plasma.
In this study, we developed a high-resolution liquid chromatography mass spectrometry method for the pharmacokinetic study of firocoxib followed by full method validation. Following a solid-phase extraction, the firocoxib and internal standard (celecoxib) were separated on an Agilent Zorbax ZDB C18 column (50mm×2.1mm i.d., 3.5μm) with a gradient elution using methanol and 0.1% aqueous formic acid. Data acquisition was performed at 25,000 resolution with the automatic gain set to 1,000,000 and the maximum injection time of 100ms. Data were acquired in full-scan mode over a mass range of 100–550Da in positive electrospray mode. Linear calibration curves were obtained over the concentration ranges of 0.5–200ng/mL and no interfering peaks were detected at the retention time of firocoxib and internal standard in blank camel plasma samples. The mean extraction recoveries of firocoxib at three concentrations of 5, 25 and 75ng/mL ranged from 92 to 104%. Coefficient of variation of intra-day and inter-day precision were both <10%. The accuracy of the method ranged from 95 to 107%. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolism of firocoxib in camels (Camelus dromedarus) (n=5) following intravenous (i.v.) administration of a dose of 0.1mgkg/body weight. The results obtained (mean±SD) were as follows: the terminal elimination half-life (t1/2β) was 5.75±2.26h, and total body clearance (ClT) was 354.1±82.6mL/kg/h. The volume of distribution at steady state (VSS) was 2344.4±238.7mL/kg. One metabolite of firocoxib was tentatively identified as desalkyl firocoxib (m/z 283). Firocoxib could be detected in plasma 3–5 days following i.v. administration in camels using a sensitive liquid chromatography high-resolution orbitrap mass spectrometry method.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2014.10.021</identifier><identifier>PMID: 25463193</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>4-Butyrolactone - administration & dosage ; 4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - metabolism ; 4-Butyrolactone - pharmacokinetics ; Administration, Intravenous ; Animals ; Automation ; Camels ; Camelus - metabolism ; Clearance ; Extraction ; Firocoxib ; High resolution ; Liquid chromatography ; Mass spectrometry ; Mass Spectrometry - instrumentation ; Mass Spectrometry - methods ; Metabolism ; Methyl alcohol ; Orbitrap ; Pharmacokinetics ; Sulfones - administration & dosage ; Sulfones - metabolism ; Sulfones - pharmacokinetics</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2015-01, Vol.974, p.17-23</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-aad0079b8aa98e53595246e6fac314d517847de6f13b2aedda39a7f94362992f3</citedby><cites>FETCH-LOGICAL-c398t-aad0079b8aa98e53595246e6fac314d517847de6f13b2aedda39a7f94362992f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S157002321400662X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25463193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wasfi, Ibrahim A.</creatorcontrib><creatorcontrib>Saeed, Hanan M.</creatorcontrib><creatorcontrib>Agha, B.A.</creatorcontrib><creatorcontrib>Kamel, Asmaa M.</creatorcontrib><creatorcontrib>Biriki, Nasreen A. Al</creatorcontrib><creatorcontrib>Neaimi, Khaled M. Al</creatorcontrib><creatorcontrib>Ali, Waleed A. Al</creatorcontrib><creatorcontrib>Sultan, Saeed Mahmoud</creatorcontrib><title>Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>•A method to quantify firocoxib by high-resolution orbitrap mass spectrometry.•Method validated with excellent recovery, linearity, precision and accuracy.•Method was successfully utilized for firocoxib pharmacokinetic analysis in camels.•The method was used to tentatively identify firocoxib metabolite in camel plasma.
In this study, we developed a high-resolution liquid chromatography mass spectrometry method for the pharmacokinetic study of firocoxib followed by full method validation. Following a solid-phase extraction, the firocoxib and internal standard (celecoxib) were separated on an Agilent Zorbax ZDB C18 column (50mm×2.1mm i.d., 3.5μm) with a gradient elution using methanol and 0.1% aqueous formic acid. Data acquisition was performed at 25,000 resolution with the automatic gain set to 1,000,000 and the maximum injection time of 100ms. Data were acquired in full-scan mode over a mass range of 100–550Da in positive electrospray mode. Linear calibration curves were obtained over the concentration ranges of 0.5–200ng/mL and no interfering peaks were detected at the retention time of firocoxib and internal standard in blank camel plasma samples. The mean extraction recoveries of firocoxib at three concentrations of 5, 25 and 75ng/mL ranged from 92 to 104%. Coefficient of variation of intra-day and inter-day precision were both <10%. The accuracy of the method ranged from 95 to 107%. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolism of firocoxib in camels (Camelus dromedarus) (n=5) following intravenous (i.v.) administration of a dose of 0.1mgkg/body weight. The results obtained (mean±SD) were as follows: the terminal elimination half-life (t1/2β) was 5.75±2.26h, and total body clearance (ClT) was 354.1±82.6mL/kg/h. The volume of distribution at steady state (VSS) was 2344.4±238.7mL/kg. One metabolite of firocoxib was tentatively identified as desalkyl firocoxib (m/z 283). Firocoxib could be detected in plasma 3–5 days following i.v. administration in camels using a sensitive liquid chromatography high-resolution orbitrap mass spectrometry method.</description><subject>4-Butyrolactone - administration & dosage</subject><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - metabolism</subject><subject>4-Butyrolactone - pharmacokinetics</subject><subject>Administration, Intravenous</subject><subject>Animals</subject><subject>Automation</subject><subject>Camels</subject><subject>Camelus - metabolism</subject><subject>Clearance</subject><subject>Extraction</subject><subject>Firocoxib</subject><subject>High resolution</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - instrumentation</subject><subject>Mass Spectrometry - methods</subject><subject>Metabolism</subject><subject>Methyl alcohol</subject><subject>Orbitrap</subject><subject>Pharmacokinetics</subject><subject>Sulfones - administration & dosage</subject><subject>Sulfones - metabolism</subject><subject>Sulfones - pharmacokinetics</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EoqXwCCAv2WTwTxLHK4QqfipVggVI7CzHvmk8xHawnYp5Fl4Wj2botiv7Hn_XV_cchF5TsqOE9u_2u72ZU_TjjhHaVm1HGH2CLukgeMNF__NpvXeCNIRxdoFe5LwnhAoi-HN0wbq251TyS_T326yT1yb-cgGKMxnrYLGHose4uOxxLps94DjhyaVo4h83Yhew0R6Wyk4FUq1L0vcQ4lYV611wuQrFxYDHA96yC3d4dndzkyDHZTs9QDBzU-KKYxpdxVfsdc44r2BKXQtKOrxEzya9ZHh1Pq_Qj08fv19_aW6_fr65_nDbGC6H0mhtCRFyHLSWA3S8kx1re-gnbThtbUfF0Apba8pHpsFazaUWk2x5z6RkE79Cb0__rin-3iAX5V02sCw6QN1J0b6jXMihOvs4ymUrB066inYn1KSYc4JJrcl5nQ6KEnWMUO3VOUJ1jPAo1whr35vziG30YB-6_mdWgfcnoCYA9w6SysZVO8G6VM1TNrpHRvwDWc20ag</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Wasfi, Ibrahim A.</creator><creator>Saeed, Hanan M.</creator><creator>Agha, B.A.</creator><creator>Kamel, Asmaa M.</creator><creator>Biriki, Nasreen A. Al</creator><creator>Neaimi, Khaled M. Al</creator><creator>Ali, Waleed A. Al</creator><creator>Sultan, Saeed Mahmoud</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20150101</creationdate><title>Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry</title><author>Wasfi, Ibrahim A. ; Saeed, Hanan M. ; Agha, B.A. ; Kamel, Asmaa M. ; Biriki, Nasreen A. Al ; Neaimi, Khaled M. Al ; Ali, Waleed A. Al ; Sultan, Saeed Mahmoud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-aad0079b8aa98e53595246e6fac314d517847de6f13b2aedda39a7f94362992f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>4-Butyrolactone - administration & dosage</topic><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - metabolism</topic><topic>4-Butyrolactone - pharmacokinetics</topic><topic>Administration, Intravenous</topic><topic>Animals</topic><topic>Automation</topic><topic>Camels</topic><topic>Camelus - metabolism</topic><topic>Clearance</topic><topic>Extraction</topic><topic>Firocoxib</topic><topic>High resolution</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - instrumentation</topic><topic>Mass Spectrometry - methods</topic><topic>Metabolism</topic><topic>Methyl alcohol</topic><topic>Orbitrap</topic><topic>Pharmacokinetics</topic><topic>Sulfones - administration & dosage</topic><topic>Sulfones - metabolism</topic><topic>Sulfones - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wasfi, Ibrahim A.</creatorcontrib><creatorcontrib>Saeed, Hanan M.</creatorcontrib><creatorcontrib>Agha, B.A.</creatorcontrib><creatorcontrib>Kamel, Asmaa M.</creatorcontrib><creatorcontrib>Biriki, Nasreen A. Al</creatorcontrib><creatorcontrib>Neaimi, Khaled M. Al</creatorcontrib><creatorcontrib>Ali, Waleed A. Al</creatorcontrib><creatorcontrib>Sultan, Saeed Mahmoud</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wasfi, Ibrahim A.</au><au>Saeed, Hanan M.</au><au>Agha, B.A.</au><au>Kamel, Asmaa M.</au><au>Biriki, Nasreen A. Al</au><au>Neaimi, Khaled M. Al</au><au>Ali, Waleed A. Al</au><au>Sultan, Saeed Mahmoud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>974</volume><spage>17</spage><epage>23</epage><pages>17-23</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>•A method to quantify firocoxib by high-resolution orbitrap mass spectrometry.•Method validated with excellent recovery, linearity, precision and accuracy.•Method was successfully utilized for firocoxib pharmacokinetic analysis in camels.•The method was used to tentatively identify firocoxib metabolite in camel plasma.
In this study, we developed a high-resolution liquid chromatography mass spectrometry method for the pharmacokinetic study of firocoxib followed by full method validation. Following a solid-phase extraction, the firocoxib and internal standard (celecoxib) were separated on an Agilent Zorbax ZDB C18 column (50mm×2.1mm i.d., 3.5μm) with a gradient elution using methanol and 0.1% aqueous formic acid. Data acquisition was performed at 25,000 resolution with the automatic gain set to 1,000,000 and the maximum injection time of 100ms. Data were acquired in full-scan mode over a mass range of 100–550Da in positive electrospray mode. Linear calibration curves were obtained over the concentration ranges of 0.5–200ng/mL and no interfering peaks were detected at the retention time of firocoxib and internal standard in blank camel plasma samples. The mean extraction recoveries of firocoxib at three concentrations of 5, 25 and 75ng/mL ranged from 92 to 104%. Coefficient of variation of intra-day and inter-day precision were both <10%. The accuracy of the method ranged from 95 to 107%. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolism of firocoxib in camels (Camelus dromedarus) (n=5) following intravenous (i.v.) administration of a dose of 0.1mgkg/body weight. The results obtained (mean±SD) were as follows: the terminal elimination half-life (t1/2β) was 5.75±2.26h, and total body clearance (ClT) was 354.1±82.6mL/kg/h. The volume of distribution at steady state (VSS) was 2344.4±238.7mL/kg. One metabolite of firocoxib was tentatively identified as desalkyl firocoxib (m/z 283). Firocoxib could be detected in plasma 3–5 days following i.v. administration in camels using a sensitive liquid chromatography high-resolution orbitrap mass spectrometry method.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25463193</pmid><doi>10.1016/j.jchromb.2014.10.021</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2015-01, Vol.974, p.17-23 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1651379887 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 4-Butyrolactone - administration & dosage 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - metabolism 4-Butyrolactone - pharmacokinetics Administration, Intravenous Animals Automation Camels Camelus - metabolism Clearance Extraction Firocoxib High resolution Liquid chromatography Mass spectrometry Mass Spectrometry - instrumentation Mass Spectrometry - methods Metabolism Methyl alcohol Orbitrap Pharmacokinetics Sulfones - administration & dosage Sulfones - metabolism Sulfones - pharmacokinetics |
| title | Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T14%3A00%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pharmacokinetics%20and%20metabolism%20study%20of%20firocoxib%20in%20camels%20after%20intravenous%20administration%20by%20using%20high-resolution%20bench-top%20orbitrap%20mass%20spectrometry&rft.jtitle=Journal%20of%20chromatography.%20B,%20Analytical%20technologies%20in%20the%20biomedical%20and%20life%20sciences&rft.au=Wasfi,%20Ibrahim%20A.&rft.date=2015-01-01&rft.volume=974&rft.spage=17&rft.epage=23&rft.pages=17-23&rft.issn=1570-0232&rft.eissn=1873-376X&rft_id=info:doi/10.1016/j.jchromb.2014.10.021&rft_dat=%3Cproquest_cross%3E1639498305%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1639498305&rft_id=info:pmid/25463193&rft_els_id=S157002321400662X&rfr_iscdi=true |