Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry
The diaminobenzidine (DAB) reaction product can be greatly intensified by incubating the reacted tissue in either nitro blue tetrazolium or tetranitro blue tetrazolium and then exposing the tissue to strong light. Epi-illumination through a microscope objective enables the photochromic intensificati...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroscience methods 1992-09, Vol.44 (2), p.217-223 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 223 |
|---|---|
| container_issue | 2 |
| container_start_page | 217 |
| container_title | Journal of neuroscience methods |
| container_volume | 44 |
| creator | Vaney, David I. |
| description | The diaminobenzidine (DAB) reaction product can be greatly intensified by incubating the reacted tissue in either nitro blue tetrazolium or tetranitro blue tetrazolium and then exposing the tissue to strong light. Epi-illumination through a microscope objective enables the photochromic intensification to be carried out under direct visual control, with optimal intensification taking only 10–30 s through a 20 × objective. Alternatively, the whole preparation can be intensified in a few minutes by passing it back and forth under a fibre light guide. The method can be used to intensify cells that have been labelled either by immunoperoxidase techniques or with intracellular tracers such as horseradish peroxidase and neurobiotin. |
| doi_str_mv | 10.1016/0165-0270(92)90013-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16507622</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0165027092900134</els_id><sourcerecordid>16507622</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-155fa79185b03bdc432305a4be2a51dda77024ff5a195d5e8dd19358acda22c03</originalsourceid><addsrcrecordid>eNp9UU2LFDEQDeKyjqv_QCEHET30mqSTSbcHQRY_FhbWg4K3UJ1UO5HuZEzSwu4_8l-a3hnWm4dQCfVe1ct7hDzj7Jwzvn1Tj2qY0OxVL173jPG2kQ_IhndaNFvdfX9INveQR-Rxzj8ZY7Jn21NyyqWWnWo35M-XXSzR7lKcvaU-FAzZj95C8THQOFLnYfYhDhhuvfMBaUKwd819im6xpZJo2WF9YsZgcSUVLAlu4-SXmWaYSn5LYb-fjmMzHWNadyWwOE3LBIlOMNSrDz8oBEf9PC8h7nxepeFca7p5Qk5GmDI-PdYz8u3jh68Xn5ur60-XF--vGiu5Lg1XagTd804NrB2cla1omQI5oADFnQOtmZDjqID3yinsnON9qzqwDoSwrD0jLw9z6_9-LZiLqftXnRAwLtlUS5neClGB8gC0KeaccDT75GdIN4YzsyZkVvvNar_phblLyMhKe36cvwwzun-kQyS1_-LYh2xhGhME6_M9TMpetX1XYe8OMKxe_PaYTLZ-9d_5hLYYF_3_dfwFoamytA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16507622</pqid></control><display><type>article</type><title>Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Vaney, David I.</creator><creatorcontrib>Vaney, David I.</creatorcontrib><description>The diaminobenzidine (DAB) reaction product can be greatly intensified by incubating the reacted tissue in either nitro blue tetrazolium or tetranitro blue tetrazolium and then exposing the tissue to strong light. Epi-illumination through a microscope objective enables the photochromic intensification to be carried out under direct visual control, with optimal intensification taking only 10–30 s through a 20 × objective. Alternatively, the whole preparation can be intensified in a few minutes by passing it back and forth under a fibre light guide. The method can be used to intensify cells that have been labelled either by immunoperoxidase techniques or with intracellular tracers such as horseradish peroxidase and neurobiotin.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/0165-0270(92)90013-4</identifier><identifier>PMID: 1474853</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Biotin ; Cats ; Choline O-Acetyltransferase - immunology ; Choline O-Acetyltransferase - metabolism ; Diaminobenzidine ; Fundamental and applied biological sciences. Psychology ; General aspects. Models. Methods ; Horseradish Peroxidase ; Hydrogen-Ion Concentration ; Immunoenzyme Techniques ; Immunohistochemistry ; Immunohistochemistry - methods ; In Vitro Techniques ; Neurobiotin ; Neurons - ultrastructure ; Nitroblue Tetrazolium - chemistry ; p-Dimethylaminoazobenzene - chemistry ; Photochromic intensification ; Rabbits ; Rats ; Retina ; Retina - cytology ; Tetrazolium salts ; Tissue Fixation ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 1992-09, Vol.44 (2), p.217-223</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-155fa79185b03bdc432305a4be2a51dda77024ff5a195d5e8dd19358acda22c03</citedby><cites>FETCH-LOGICAL-c417t-155fa79185b03bdc432305a4be2a51dda77024ff5a195d5e8dd19358acda22c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0165027092900134$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4495398$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1474853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vaney, David I.</creatorcontrib><title>Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>The diaminobenzidine (DAB) reaction product can be greatly intensified by incubating the reacted tissue in either nitro blue tetrazolium or tetranitro blue tetrazolium and then exposing the tissue to strong light. Epi-illumination through a microscope objective enables the photochromic intensification to be carried out under direct visual control, with optimal intensification taking only 10–30 s through a 20 × objective. Alternatively, the whole preparation can be intensified in a few minutes by passing it back and forth under a fibre light guide. The method can be used to intensify cells that have been labelled either by immunoperoxidase techniques or with intracellular tracers such as horseradish peroxidase and neurobiotin.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotin</subject><subject>Cats</subject><subject>Choline O-Acetyltransferase - immunology</subject><subject>Choline O-Acetyltransferase - metabolism</subject><subject>Diaminobenzidine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Horseradish Peroxidase</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunoenzyme Techniques</subject><subject>Immunohistochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>In Vitro Techniques</subject><subject>Neurobiotin</subject><subject>Neurons - ultrastructure</subject><subject>Nitroblue Tetrazolium - chemistry</subject><subject>p-Dimethylaminoazobenzene - chemistry</subject><subject>Photochromic intensification</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Retina</subject><subject>Retina - cytology</subject><subject>Tetrazolium salts</subject><subject>Tissue Fixation</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU2LFDEQDeKyjqv_QCEHET30mqSTSbcHQRY_FhbWg4K3UJ1UO5HuZEzSwu4_8l-a3hnWm4dQCfVe1ct7hDzj7Jwzvn1Tj2qY0OxVL173jPG2kQ_IhndaNFvdfX9INveQR-Rxzj8ZY7Jn21NyyqWWnWo35M-XXSzR7lKcvaU-FAzZj95C8THQOFLnYfYhDhhuvfMBaUKwd819im6xpZJo2WF9YsZgcSUVLAlu4-SXmWaYSn5LYb-fjmMzHWNadyWwOE3LBIlOMNSrDz8oBEf9PC8h7nxepeFca7p5Qk5GmDI-PdYz8u3jh68Xn5ur60-XF--vGiu5Lg1XagTd804NrB2cla1omQI5oADFnQOtmZDjqID3yinsnON9qzqwDoSwrD0jLw9z6_9-LZiLqftXnRAwLtlUS5neClGB8gC0KeaccDT75GdIN4YzsyZkVvvNar_phblLyMhKe36cvwwzun-kQyS1_-LYh2xhGhME6_M9TMpetX1XYe8OMKxe_PaYTLZ-9d_5hLYYF_3_dfwFoamytA</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Vaney, David I.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19920901</creationdate><title>Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry</title><author>Vaney, David I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-155fa79185b03bdc432305a4be2a51dda77024ff5a195d5e8dd19358acda22c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotin</topic><topic>Cats</topic><topic>Choline O-Acetyltransferase - immunology</topic><topic>Choline O-Acetyltransferase - metabolism</topic><topic>Diaminobenzidine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Horseradish Peroxidase</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunoenzyme Techniques</topic><topic>Immunohistochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>In Vitro Techniques</topic><topic>Neurobiotin</topic><topic>Neurons - ultrastructure</topic><topic>Nitroblue Tetrazolium - chemistry</topic><topic>p-Dimethylaminoazobenzene - chemistry</topic><topic>Photochromic intensification</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Retina</topic><topic>Retina - cytology</topic><topic>Tetrazolium salts</topic><topic>Tissue Fixation</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vaney, David I.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaney, David I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>44</volume><issue>2</issue><spage>217</spage><epage>223</epage><pages>217-223</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>The diaminobenzidine (DAB) reaction product can be greatly intensified by incubating the reacted tissue in either nitro blue tetrazolium or tetranitro blue tetrazolium and then exposing the tissue to strong light. Epi-illumination through a microscope objective enables the photochromic intensification to be carried out under direct visual control, with optimal intensification taking only 10–30 s through a 20 × objective. Alternatively, the whole preparation can be intensified in a few minutes by passing it back and forth under a fibre light guide. The method can be used to intensify cells that have been labelled either by immunoperoxidase techniques or with intracellular tracers such as horseradish peroxidase and neurobiotin.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1474853</pmid><doi>10.1016/0165-0270(92)90013-4</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-0270 |
| ispartof | Journal of neuroscience methods, 1992-09, Vol.44 (2), p.217-223 |
| issn | 0165-0270 1872-678X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16507622 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Biotin Cats Choline O-Acetyltransferase - immunology Choline O-Acetyltransferase - metabolism Diaminobenzidine Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Horseradish Peroxidase Hydrogen-Ion Concentration Immunoenzyme Techniques Immunohistochemistry Immunohistochemistry - methods In Vitro Techniques Neurobiotin Neurons - ultrastructure Nitroblue Tetrazolium - chemistry p-Dimethylaminoazobenzene - chemistry Photochromic intensification Rabbits Rats Retina Retina - cytology Tetrazolium salts Tissue Fixation Vertebrates: nervous system and sense organs |
| title | Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T22%3A03%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Photochromic%20intensification%20of%20diaminobenzidine%20reaction%20product%20in%20the%20presence%20of%20tetrazolium%20salts:%20applications%20for%20intracellular%20labelling%20and%20immunohistochemistry&rft.jtitle=Journal%20of%20neuroscience%20methods&rft.au=Vaney,%20David%20I.&rft.date=1992-09-01&rft.volume=44&rft.issue=2&rft.spage=217&rft.epage=223&rft.pages=217-223&rft.issn=0165-0270&rft.eissn=1872-678X&rft.coden=JNMEDT&rft_id=info:doi/10.1016/0165-0270(92)90013-4&rft_dat=%3Cproquest_cross%3E16507622%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16507622&rft_id=info:pmid/1474853&rft_els_id=0165027092900134&rfr_iscdi=true |