Evaluation of a tissue homogenization technique that isolates nuclei for the in vivo single cell gel electrophoresis (comet) assay: a collaborative study by five laboratories

We evaluated a tissue homogenization technique that isolates nuclei for use in the in vivo comet assay. Five laboratories independently tested the technique using the liver, kidney, lung, spleen, and bone marrow of untreated and mutagen-treated male CD-1 mice. The direct mutagen methylmethanesulfona...

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Veröffentlicht in:	Mutation research 1998-10, Vol.418 (2), p.131-140
Hauptverfasser:	Miyamae, Youichi, Yamamoto, Mika, Sasaki, Yu.F, Kobayashi, Hiroshi, Igarashi-Soga, Miyuki, Shimoi, Kayoko, Hayashi, Makoto
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Bone Marrow - drug effects Bone Marrow - ultrastructure Cell Fractionation - methods Cell Nucleus - drug effects Cell Nucleus - genetics Chemical mutagenesis Comet assay Diethylnitrosamine - toxicity DNA Damage - genetics DNA lesion Electrophoresis, Agar Gel - methods Ethidium - chemistry Fluorescent Dyes - chemistry In vivo Kidney - drug effects Kidney - ultrastructure Liver - drug effects Liver - ultrastructure Lung - drug effects Lung - ultrastructure Male Medical sciences Methyl Methanesulfonate - toxicity Mice Microscopy, Fluorescence Mouse Mutagens - toxicity Reproducibility of Results Single cell gel electrophoresis (SCG) assay Spleen - drug effects Spleen - ultrastructure Toxicology
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