Changes in activity of soluble and immobilized peroxidases after treatment by various proteases and some metal ions
The proteases used were cabbage serine proteinase, trypsin, proteinase K, papain, pepsin, pronase and carboxypeptidase Y. The metal ions used were Mg, Mn, Cu, Hg, Pb and Ag. The incubation of cabbage peroxidase, soluble or immobilized, with seven different proteases and six different metal ions duri...
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| Veröffentlicht in: | Journal of molecular catalysis 1993-03, Vol.80 (1), p.117-125 |
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| container_end_page | 125 |
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| container_issue | 1 |
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| container_title | Journal of molecular catalysis |
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| creator | Grzywnowicz, Krzysztof Greppin, Hubert Brzyska, Maria Łobarzewski, Jerzy |
| description | The proteases used were cabbage serine proteinase, trypsin, proteinase K, papain, pepsin, pronase and carboxypeptidase Y. The metal ions used were Mg, Mn, Cu, Hg, Pb and Ag. The incubation of cabbage peroxidase, soluble or immobilized, with seven different proteases and six different metal ions during 1 to 2 hours caused an increase in enzyme activity up to 250%. Longer incubation times of the peroxidase with the proteases caused a decrease of the enzyme activity but the immobilization stabilized its activity, despite the action of proteases. Especially evident was activation (from 50% to 200%) by serine proteinases, including the cabbage serine proteinase, in the presence of Pb, Hg, Cu and Mg ions after one to two hours of incubation. The observed changes in the peroxidase activities after protease and metal ion treatment may resemble those in nature or in enzymatic bioreactors. |
| doi_str_mv | 10.1016/0304-5102(93)87114-N |
| format | Article |
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The metal ions used were Mg, Mn, Cu, Hg, Pb and Ag. The incubation of cabbage peroxidase, soluble or immobilized, with seven different proteases and six different metal ions during 1 to 2 hours caused an increase in enzyme activity up to 250%. Longer incubation times of the peroxidase with the proteases caused a decrease of the enzyme activity but the immobilization stabilized its activity, despite the action of proteases. Especially evident was activation (from 50% to 200%) by serine proteinases, including the cabbage serine proteinase, in the presence of Pb, Hg, Cu and Mg ions after one to two hours of incubation. The observed changes in the peroxidase activities after protease and metal ion treatment may resemble those in nature or in enzymatic bioreactors.</description><identifier>ISSN: 0304-5102</identifier><identifier>DOI: 10.1016/0304-5102(93)87114-N</identifier><identifier>CODEN: JMCADS</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; enzyme activity ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. 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The observed changes in the peroxidase activities after protease and metal ion treatment may resemble those in nature or in enzymatic bioreactors.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>enzyme activity</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>metal ions</topic><topic>Oxidoreductases</topic><topic>peroxidases, proteases</topic><toplevel>online_resources</toplevel><creatorcontrib>Grzywnowicz, Krzysztof</creatorcontrib><creatorcontrib>Greppin, Hubert</creatorcontrib><creatorcontrib>Brzyska, Maria</creatorcontrib><creatorcontrib>Łobarzewski, Jerzy</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of molecular catalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grzywnowicz, Krzysztof</au><au>Greppin, Hubert</au><au>Brzyska, Maria</au><au>Łobarzewski, Jerzy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in activity of soluble and immobilized peroxidases after treatment by various proteases and some metal ions</atitle><jtitle>Journal of molecular catalysis</jtitle><date>1993-03-03</date><risdate>1993</risdate><volume>80</volume><issue>1</issue><spage>117</spage><epage>125</epage><pages>117-125</pages><issn>0304-5102</issn><coden>JMCADS</coden><abstract>The proteases used were cabbage serine proteinase, trypsin, proteinase K, papain, pepsin, pronase and carboxypeptidase Y. 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| ispartof | Journal of molecular catalysis, 1993-03, Vol.80 (1), p.117-125 |
| issn | 0304-5102 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences enzyme activity Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology metal ions Oxidoreductases peroxidases, proteases |
| title | Changes in activity of soluble and immobilized peroxidases after treatment by various proteases and some metal ions |
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