Excystment In vitro of Notocotylus attenuatus (Trematoda: Notocotyloidea) Metacercariae
Optimum in vitro excystation of Notocotylus attenuatus (Rudolphi, 1809) metacercarial cysts occurred following pretreatment of the cyst for 2 hr in N,N′,N′-polyoxyethylene(10)-N-tallow-1,3-diamino propane/water solution (1:2), a second pretreatment (15 min) in 1.0% HC1 acid-1.0% pepsin (pH 2.0), and...
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| Veröffentlicht in: | The Journal of parasitology 1993-02, Vol.79 (1), p.92-97 |
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| container_title | The Journal of parasitology |
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| creator | Graczyk, Thaddeus K. Shift, Clive J. |
| description | Optimum in vitro excystation of Notocotylus attenuatus (Rudolphi, 1809) metacercarial cysts occurred following pretreatment of the cyst for 2 hr in N,N′,N′-polyoxyethylene(10)-N-tallow-1,3-diamino propane/water solution (1:2), a second pretreatment (15 min) in 1.0% HC1 acid-1.0% pepsin (pH 2.0), and then 2 hr treatment in an excystation medium containing 0.5% trypsin plus 0.5% bile salts in Earle's balanced salt solution adjusted with 7.5% NaHCO3to pH 7.8-8.0. The rate of excystation was rapid and reached 71.7% by 1 hr, increasing to 78.7% after 2 hr. Storage of metacercarial cysts for up to 12 wk at 4 C in Locke's 1:1 solution did not alter cyst viability. In vitro excystation was influenced by structures in the mucoid envelope of the cyst, including a funnel-shaped cavity and the narrow tube. This is the first report of N. attenuatus from North America. |
| doi_str_mv | 10.2307/3283283 |
| format | Article |
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The rate of excystation was rapid and reached 71.7% by 1 hr, increasing to 78.7% after 2 hr. Storage of metacercarial cysts for up to 12 wk at 4 C in Locke's 1:1 solution did not alter cyst viability. In vitro excystation was influenced by structures in the mucoid envelope of the cyst, including a funnel-shaped cavity and the narrow tube. This is the first report of N. attenuatus from North America.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.2307/3283283</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Bile acids ; Biological and medical sciences ; Cysts ; Detergents ; Freshwater ; Fundamental and applied biological sciences. Psychology ; General aspects and techniques. Study of several systematic groups. 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The rate of excystation was rapid and reached 71.7% by 1 hr, increasing to 78.7% after 2 hr. Storage of metacercarial cysts for up to 12 wk at 4 C in Locke's 1:1 solution did not alter cyst viability. In vitro excystation was influenced by structures in the mucoid envelope of the cyst, including a funnel-shaped cavity and the narrow tube. This is the first report of N. attenuatus from North America.</description><subject>Bile acids</subject><subject>Biological and medical sciences</subject><subject>Cysts</subject><subject>Detergents</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Immunology</subject><subject>Invertebrates</subject><subject>Larvae</subject><subject>Life Cycle</subject><subject>Metacercariae</subject><subject>Miscellaneous techniques</subject><subject>Notocotylus attenuatus</subject><subject>Parasites</subject><subject>Parasitology</subject><subject>Pretreatment</subject><subject>Techniques</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNp10E1Lw0AQBuBFFKxV_As5iB-H6H5kNxtvpVQtVL1UPIbJZgIpSbbubsT-e1Na7EmYYebw8B5eQi4ZveeCpg-C6-0ckRHLRBpzkchjMqKU81iITJ6SM-9XlFI57Ih8zn7MxocWuxDNu-i7Ds5GtorebLDGhk3T-whCwK6HMLy3S4ctBFvC44HYukS4i14xgEFnwNWA5-Skgsbjxf6OycfTbDl9iRfvz_PpZBEbprMQp4KnBU8oLzOJoqAio6WqNPCyyAxHzSVXTBgtaWIKqNLUABdK6FQjBYZcjMn1Lnft7FePPuRt7Q02DXRoe58zJSnTKhngzQ4aZ713WOVrV7fgNjmj-ba4fF_cIK_2keANNJWDztT-jydKSZaoA1v5YN2_ab_4FHeO</recordid><startdate>19930201</startdate><enddate>19930201</enddate><creator>Graczyk, Thaddeus K.</creator><creator>Shift, Clive J.</creator><general>American Society of Parasitologists</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>19930201</creationdate><title>Excystment In vitro of Notocotylus attenuatus (Trematoda: Notocotyloidea) Metacercariae</title><author>Graczyk, Thaddeus K. ; Shift, Clive J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c189t-7327b2402d95e3b0390d6f8a2db9c2e8252613c8504cbaf77ca2363878e0a1e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bile acids</topic><topic>Biological and medical sciences</topic><topic>Cysts</topic><topic>Detergents</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Immunology</topic><topic>Invertebrates</topic><topic>Larvae</topic><topic>Life Cycle</topic><topic>Metacercariae</topic><topic>Miscellaneous techniques</topic><topic>Notocotylus attenuatus</topic><topic>Parasites</topic><topic>Parasitology</topic><topic>Pretreatment</topic><topic>Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Graczyk, Thaddeus K.</creatorcontrib><creatorcontrib>Shift, Clive J.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Graczyk, Thaddeus K.</au><au>Shift, Clive J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Excystment In vitro of Notocotylus attenuatus (Trematoda: Notocotyloidea) Metacercariae</atitle><jtitle>The Journal of parasitology</jtitle><date>1993-02-01</date><risdate>1993</risdate><volume>79</volume><issue>1</issue><spage>92</spage><epage>97</epage><pages>92-97</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>Optimum in vitro excystation of Notocotylus attenuatus (Rudolphi, 1809) metacercarial cysts occurred following pretreatment of the cyst for 2 hr in N,N′,N′-polyoxyethylene(10)-N-tallow-1,3-diamino propane/water solution (1:2), a second pretreatment (15 min) in 1.0% HC1 acid-1.0% pepsin (pH 2.0), and then 2 hr treatment in an excystation medium containing 0.5% trypsin plus 0.5% bile salts in Earle's balanced salt solution adjusted with 7.5% NaHCO3to pH 7.8-8.0. The rate of excystation was rapid and reached 71.7% by 1 hr, increasing to 78.7% after 2 hr. Storage of metacercarial cysts for up to 12 wk at 4 C in Locke's 1:1 solution did not alter cyst viability. In vitro excystation was influenced by structures in the mucoid envelope of the cyst, including a funnel-shaped cavity and the narrow tube. This is the first report of N. attenuatus from North America.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><doi>10.2307/3283283</doi><tpages>6</tpages></addata></record> |
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| ispartof | The Journal of parasitology, 1993-02, Vol.79 (1), p.92-97 |
| issn | 0022-3395 1937-2345 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16501864 |
| source | Jstor Complete Legacy |
| subjects | Bile acids Biological and medical sciences Cysts Detergents Freshwater Fundamental and applied biological sciences. Psychology General aspects and techniques. Study of several systematic groups. Models Immunology Invertebrates Larvae Life Cycle Metacercariae Miscellaneous techniques Notocotylus attenuatus Parasites Parasitology Pretreatment Techniques |
| title | Excystment In vitro of Notocotylus attenuatus (Trematoda: Notocotyloidea) Metacercariae |
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