ATP‐induced chondrocalcinosis
Objective. To determine whether adult articular cartilage mineralizes in the presence of ATP. Methods. Intact adult porcine articular cartilage and monolayers of chondrocytes were cultured in physiologic media containing ATP, and mineralization was measured as retention of 45Ca. Cartilage was analyz...
Gespeichert in:
| Veröffentlicht in: | Arthritis and rheumatism 1992-12, Vol.35 (12), p.1520-1525 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1525 |
|---|---|
| container_issue | 12 |
| container_start_page | 1520 |
| container_title | Arthritis and rheumatism |
| container_volume | 35 |
| creator | Ryan, Lawrence M. Kurup, Indira V. Derfus, Beth A. Kushnaryov, Vladimir M. |
| description | Objective. To determine whether adult articular cartilage mineralizes in the presence of ATP.
Methods. Intact adult porcine articular cartilage and monolayers of chondrocytes were cultured in physiologic media containing ATP, and mineralization was measured as retention of 45Ca. Cartilage was analyzed by electron microscopy.
Results. Articular cartilage sequestered 45Ca when incubated with 100 γM ATP. Use of the ATP analog α,β‐methylene ATP did not promote mineralization and addition of pyrophosphatase inhibited mineralization, indicating that hydrolysis of ATP to AMP and inorganic pyrophosphate is necessary for the process to occur. Mineral was concentrated in articular cartilage vesicles in the perichondral area.
Conclusion. Adult articular cartilage mineralizes in the presence of ATP, in a manner similar to that found with isolated matrix or articular cartilage vesicles. This supports the notion that these structures have a role in chondrocalcinosis. |
| doi_str_mv | 10.1002/art.1780351216 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16498050</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16498050</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4666-3ac594b76446a091e64c9575ec974b826310b432be35c77f0ebf77540021c2c23</originalsourceid><addsrcrecordid>eNqFkE1LAzEQhoMotVav3kQP4m1rJp-bYyl-QUGReg7ZbBYj292adCm9-RP8jf4SI1usN0_DMM-8886L0CngMWBMrk1YjUHmmHIgIPbQEDhRGQYK-2iIMWYZ5QoO0VGMb6kllNMBGgCTBIgaovPJ_Onr49M3ZWddeWFf26YMrTW19U0bfTxGB5WpozvZ1hF6ub2ZT--z2ePdw3QyyywTQmTUWK5YIQVjwmAFTjCruOTOKsmKnAgKuGCUFI5yK2WFXVFJyVkyBJZYQkfoqtddhva9c3GlFz5aV9emcW0XNQimcsxxAsc9aEMbY3CVXga_MGGjAeufRHRKRO8SSQtnW-WuWLhyh_cRpPnldm5i-rsKprE-_mKM5bnMecJUj6197Tb_HNWT5_kfC99zyXd1</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16498050</pqid></control><display><type>article</type><title>ATP‐induced chondrocalcinosis</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><creator>Ryan, Lawrence M. ; Kurup, Indira V. ; Derfus, Beth A. ; Kushnaryov, Vladimir M.</creator><creatorcontrib>Ryan, Lawrence M. ; Kurup, Indira V. ; Derfus, Beth A. ; Kushnaryov, Vladimir M.</creatorcontrib><description>Objective. To determine whether adult articular cartilage mineralizes in the presence of ATP.
Methods. Intact adult porcine articular cartilage and monolayers of chondrocytes were cultured in physiologic media containing ATP, and mineralization was measured as retention of 45Ca. Cartilage was analyzed by electron microscopy.
Results. Articular cartilage sequestered 45Ca when incubated with 100 γM ATP. Use of the ATP analog α,β‐methylene ATP did not promote mineralization and addition of pyrophosphatase inhibited mineralization, indicating that hydrolysis of ATP to AMP and inorganic pyrophosphate is necessary for the process to occur. Mineral was concentrated in articular cartilage vesicles in the perichondral area.
Conclusion. Adult articular cartilage mineralizes in the presence of ATP, in a manner similar to that found with isolated matrix or articular cartilage vesicles. This supports the notion that these structures have a role in chondrocalcinosis.</description><identifier>ISSN: 0004-3591</identifier><identifier>EISSN: 1529-0131</identifier><identifier>DOI: 10.1002/art.1780351216</identifier><identifier>PMID: 1472129</identifier><identifier>CODEN: ARHEAW</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Adenosine Triphosphate - pharmacology ; Animals ; Biological and medical sciences ; Calcification, Physiologic - drug effects ; Calcium - metabolism ; Calcium Phosphates - pharmacology ; Calcium Pyrophosphate - pharmacology ; Calcium Radioisotopes ; Cartilage, Articular - metabolism ; Cartilage, Articular - pathology ; Cartilage, Articular - ultrastructure ; Cells, Cultured ; Chondrocalcinosis - chemically induced ; Chondrocalcinosis - metabolism ; Chondrocalcinosis - pathology ; Diseases of the osteoarticular system ; Hydrolysis ; Medical sciences ; Microscopy, Electron ; Miscellaneous. Osteoarticular involvement in other diseases</subject><ispartof>Arthritis and rheumatism, 1992-12, Vol.35 (12), p.1520-1525</ispartof><rights>Copyright © 1992 American College of Rheumatology</rights><rights>1993 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4666-3ac594b76446a091e64c9575ec974b826310b432be35c77f0ebf77540021c2c23</citedby><cites>FETCH-LOGICAL-c4666-3ac594b76446a091e64c9575ec974b826310b432be35c77f0ebf77540021c2c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fart.1780351216$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fart.1780351216$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4488785$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1472129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ryan, Lawrence M.</creatorcontrib><creatorcontrib>Kurup, Indira V.</creatorcontrib><creatorcontrib>Derfus, Beth A.</creatorcontrib><creatorcontrib>Kushnaryov, Vladimir M.</creatorcontrib><title>ATP‐induced chondrocalcinosis</title><title>Arthritis and rheumatism</title><addtitle>Arthritis Rheum</addtitle><description>Objective. To determine whether adult articular cartilage mineralizes in the presence of ATP.
Methods. Intact adult porcine articular cartilage and monolayers of chondrocytes were cultured in physiologic media containing ATP, and mineralization was measured as retention of 45Ca. Cartilage was analyzed by electron microscopy.
Results. Articular cartilage sequestered 45Ca when incubated with 100 γM ATP. Use of the ATP analog α,β‐methylene ATP did not promote mineralization and addition of pyrophosphatase inhibited mineralization, indicating that hydrolysis of ATP to AMP and inorganic pyrophosphate is necessary for the process to occur. Mineral was concentrated in articular cartilage vesicles in the perichondral area.
Conclusion. Adult articular cartilage mineralizes in the presence of ATP, in a manner similar to that found with isolated matrix or articular cartilage vesicles. This supports the notion that these structures have a role in chondrocalcinosis.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcification, Physiologic - drug effects</subject><subject>Calcium - metabolism</subject><subject>Calcium Phosphates - pharmacology</subject><subject>Calcium Pyrophosphate - pharmacology</subject><subject>Calcium Radioisotopes</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cartilage, Articular - pathology</subject><subject>Cartilage, Articular - ultrastructure</subject><subject>Cells, Cultured</subject><subject>Chondrocalcinosis - chemically induced</subject><subject>Chondrocalcinosis - metabolism</subject><subject>Chondrocalcinosis - pathology</subject><subject>Diseases of the osteoarticular system</subject><subject>Hydrolysis</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Miscellaneous. Osteoarticular involvement in other diseases</subject><issn>0004-3591</issn><issn>1529-0131</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotVav3kQP4m1rJp-bYyl-QUGReg7ZbBYj292adCm9-RP8jf4SI1usN0_DMM-8886L0CngMWBMrk1YjUHmmHIgIPbQEDhRGQYK-2iIMWYZ5QoO0VGMb6kllNMBGgCTBIgaovPJ_Onr49M3ZWddeWFf26YMrTW19U0bfTxGB5WpozvZ1hF6ub2ZT--z2ePdw3QyyywTQmTUWK5YIQVjwmAFTjCruOTOKsmKnAgKuGCUFI5yK2WFXVFJyVkyBJZYQkfoqtddhva9c3GlFz5aV9emcW0XNQimcsxxAsc9aEMbY3CVXga_MGGjAeufRHRKRO8SSQtnW-WuWLhyh_cRpPnldm5i-rsKprE-_mKM5bnMecJUj6197Tb_HNWT5_kfC99zyXd1</recordid><startdate>199212</startdate><enddate>199212</enddate><creator>Ryan, Lawrence M.</creator><creator>Kurup, Indira V.</creator><creator>Derfus, Beth A.</creator><creator>Kushnaryov, Vladimir M.</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope></search><sort><creationdate>199212</creationdate><title>ATP‐induced chondrocalcinosis</title><author>Ryan, Lawrence M. ; Kurup, Indira V. ; Derfus, Beth A. ; Kushnaryov, Vladimir M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4666-3ac594b76446a091e64c9575ec974b826310b432be35c77f0ebf77540021c2c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcification, Physiologic - drug effects</topic><topic>Calcium - metabolism</topic><topic>Calcium Phosphates - pharmacology</topic><topic>Calcium Pyrophosphate - pharmacology</topic><topic>Calcium Radioisotopes</topic><topic>Cartilage, Articular - metabolism</topic><topic>Cartilage, Articular - pathology</topic><topic>Cartilage, Articular - ultrastructure</topic><topic>Cells, Cultured</topic><topic>Chondrocalcinosis - chemically induced</topic><topic>Chondrocalcinosis - metabolism</topic><topic>Chondrocalcinosis - pathology</topic><topic>Diseases of the osteoarticular system</topic><topic>Hydrolysis</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Miscellaneous. Osteoarticular involvement in other diseases</topic><toplevel>online_resources</toplevel><creatorcontrib>Ryan, Lawrence M.</creatorcontrib><creatorcontrib>Kurup, Indira V.</creatorcontrib><creatorcontrib>Derfus, Beth A.</creatorcontrib><creatorcontrib>Kushnaryov, Vladimir M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Arthritis and rheumatism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ryan, Lawrence M.</au><au>Kurup, Indira V.</au><au>Derfus, Beth A.</au><au>Kushnaryov, Vladimir M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ATP‐induced chondrocalcinosis</atitle><jtitle>Arthritis and rheumatism</jtitle><addtitle>Arthritis Rheum</addtitle><date>1992-12</date><risdate>1992</risdate><volume>35</volume><issue>12</issue><spage>1520</spage><epage>1525</epage><pages>1520-1525</pages><issn>0004-3591</issn><eissn>1529-0131</eissn><coden>ARHEAW</coden><abstract>Objective. To determine whether adult articular cartilage mineralizes in the presence of ATP.
Methods. Intact adult porcine articular cartilage and monolayers of chondrocytes were cultured in physiologic media containing ATP, and mineralization was measured as retention of 45Ca. Cartilage was analyzed by electron microscopy.
Results. Articular cartilage sequestered 45Ca when incubated with 100 γM ATP. Use of the ATP analog α,β‐methylene ATP did not promote mineralization and addition of pyrophosphatase inhibited mineralization, indicating that hydrolysis of ATP to AMP and inorganic pyrophosphate is necessary for the process to occur. Mineral was concentrated in articular cartilage vesicles in the perichondral area.
Conclusion. Adult articular cartilage mineralizes in the presence of ATP, in a manner similar to that found with isolated matrix or articular cartilage vesicles. This supports the notion that these structures have a role in chondrocalcinosis.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>1472129</pmid><doi>10.1002/art.1780351216</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0004-3591 |
| ispartof | Arthritis and rheumatism, 1992-12, Vol.35 (12), p.1520-1525 |
| issn | 0004-3591 1529-0131 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16498050 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - pharmacology Animals Biological and medical sciences Calcification, Physiologic - drug effects Calcium - metabolism Calcium Phosphates - pharmacology Calcium Pyrophosphate - pharmacology Calcium Radioisotopes Cartilage, Articular - metabolism Cartilage, Articular - pathology Cartilage, Articular - ultrastructure Cells, Cultured Chondrocalcinosis - chemically induced Chondrocalcinosis - metabolism Chondrocalcinosis - pathology Diseases of the osteoarticular system Hydrolysis Medical sciences Microscopy, Electron Miscellaneous. Osteoarticular involvement in other diseases |
| title | ATP‐induced chondrocalcinosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T13%3A16%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=ATP%E2%80%90induced%20chondrocalcinosis&rft.jtitle=Arthritis%20and%20rheumatism&rft.au=Ryan,%20Lawrence%20M.&rft.date=1992-12&rft.volume=35&rft.issue=12&rft.spage=1520&rft.epage=1525&rft.pages=1520-1525&rft.issn=0004-3591&rft.eissn=1529-0131&rft.coden=ARHEAW&rft_id=info:doi/10.1002/art.1780351216&rft_dat=%3Cproquest_cross%3E16498050%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16498050&rft_id=info:pmid/1472129&rfr_iscdi=true |