Pleiotrophin stimulates fibroblasts and endothelial and epithelial cells and is expressed in human cancer
Previously we reported the purification of the heparin-binding growth factor pleiotrophin (PTN) from supernatants of the human breast cancer cell line MDA-MB-231. To investigate further the biological activities of PTN and its potential role in cancer, we cloned a PTN cDNA and expressed the gene in...
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| Veröffentlicht in: | The Journal of biological chemistry 1992-12, Vol.267 (36), p.25889-25897 |
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| container_issue | 36 |
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| creator | WENJING FANG HARTMANN, N CHOW, D. T TATE RIEGEL, A WELLSTEIN, A |
| description | Previously we reported the purification of the heparin-binding growth factor pleiotrophin (PTN) from supernatants of the human
breast cancer cell line MDA-MB-231. To investigate further the biological activities of PTN and its potential role in cancer,
we cloned a PTN cDNA and expressed the gene in a human kidney and in a human adrenal carcinoma cell line (SW-13). The supernatants
harvested from cells transfected with PTN contained a heparin-binding specific protein of an apparent molecular mass of 18
kDa. These supernatants stimulated the proliferation of endothelial cells as well as the anchorage-independent growth of SW-13
cells and of normal rat kidney fibroblasts. Furthermore, SW-13 cells transfected with PTN acquired autonomous growth in soft
agar and were tumorigenic in athymic nude mice. In contrast to these results with PTN from human cells, PTN obtained from
insect cells (Sf9) using recombinant baculovirus as a vector was biologically inactive. We detected high levels of PTN mRNA
in 16 of 27 primary human breast cancer samples (62%) as well as in 8 of 8 carcinogen-induced rat mammary tumors. Furthermore,
9 of 34 human tumor cell lines of different origin showed detectable PTN mRNA. We conclude that PTN may function as a tumor
growth and angiogenesis factor in addition to its role during embryonic development. |
| doi_str_mv | 10.1016/s0021-9258(18)35692-8 |
| format | Article |
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breast cancer cell line MDA-MB-231. To investigate further the biological activities of PTN and its potential role in cancer,
we cloned a PTN cDNA and expressed the gene in a human kidney and in a human adrenal carcinoma cell line (SW-13). The supernatants
harvested from cells transfected with PTN contained a heparin-binding specific protein of an apparent molecular mass of 18
kDa. These supernatants stimulated the proliferation of endothelial cells as well as the anchorage-independent growth of SW-13
cells and of normal rat kidney fibroblasts. Furthermore, SW-13 cells transfected with PTN acquired autonomous growth in soft
agar and were tumorigenic in athymic nude mice. In contrast to these results with PTN from human cells, PTN obtained from
insect cells (Sf9) using recombinant baculovirus as a vector was biologically inactive. We detected high levels of PTN mRNA
in 16 of 27 primary human breast cancer samples (62%) as well as in 8 of 8 carcinogen-induced rat mammary tumors. Furthermore,
9 of 34 human tumor cell lines of different origin showed detectable PTN mRNA. We conclude that PTN may function as a tumor
growth and angiogenesis factor in addition to its role during embryonic development.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(18)35692-8</identifier><identifier>PMID: 1464602</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Adrenal Gland Neoplasms ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; carcinoma ; Carrier Proteins ; Cell Division - drug effects ; Cell Line ; Cloning, Molecular ; Cytokines - genetics ; Cytokines - pharmacology ; Cytokines - physiology ; embryogenesis ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; expression ; Female ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Genetic Vectors ; Host-tumor relations. Immunology. Biological markers ; Humans ; Insecta ; Kidney ; Male ; man ; Medical sciences ; Mitogens - physiology ; Molecular Sequence Data ; Neoplasms - genetics ; Oligodeoxyribonucleotides ; pleiotrophin ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; role ; Transfection ; Tumor Cells, Cultured ; Tumors</subject><ispartof>The Journal of biological chemistry, 1992-12, Vol.267 (36), p.25889-25897</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-45230450af09605130ae814fa49a90ef999aa6cbcbc4e554cb6ef378ca83b8cc3</citedby><cites>FETCH-LOGICAL-c506t-45230450af09605130ae814fa49a90ef999aa6cbcbc4e554cb6ef378ca83b8cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4554834$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1464602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WENJING FANG</creatorcontrib><creatorcontrib>HARTMANN, N</creatorcontrib><creatorcontrib>CHOW, D. T</creatorcontrib><creatorcontrib>TATE RIEGEL, A</creatorcontrib><creatorcontrib>WELLSTEIN, A</creatorcontrib><title>Pleiotrophin stimulates fibroblasts and endothelial and epithelial cells and is expressed in human cancer</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Previously we reported the purification of the heparin-binding growth factor pleiotrophin (PTN) from supernatants of the human
breast cancer cell line MDA-MB-231. To investigate further the biological activities of PTN and its potential role in cancer,
we cloned a PTN cDNA and expressed the gene in a human kidney and in a human adrenal carcinoma cell line (SW-13). The supernatants
harvested from cells transfected with PTN contained a heparin-binding specific protein of an apparent molecular mass of 18
kDa. These supernatants stimulated the proliferation of endothelial cells as well as the anchorage-independent growth of SW-13
cells and of normal rat kidney fibroblasts. Furthermore, SW-13 cells transfected with PTN acquired autonomous growth in soft
agar and were tumorigenic in athymic nude mice. In contrast to these results with PTN from human cells, PTN obtained from
insect cells (Sf9) using recombinant baculovirus as a vector was biologically inactive. We detected high levels of PTN mRNA
in 16 of 27 primary human breast cancer samples (62%) as well as in 8 of 8 carcinogen-induced rat mammary tumors. Furthermore,
9 of 34 human tumor cell lines of different origin showed detectable PTN mRNA. We conclude that PTN may function as a tumor
growth and angiogenesis factor in addition to its role during embryonic development.</description><subject>Adrenal Gland Neoplasms</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>carcinoma</subject><subject>Carrier Proteins</subject><subject>Cell Division - drug effects</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>Cytokines - genetics</subject><subject>Cytokines - pharmacology</subject><subject>Cytokines - physiology</subject><subject>embryogenesis</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>expression</subject><subject>Female</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Genetic Vectors</subject><subject>Host-tumor relations. Immunology. Biological markers</subject><subject>Humans</subject><subject>Insecta</subject><subject>Kidney</subject><subject>Male</subject><subject>man</subject><subject>Medical sciences</subject><subject>Mitogens - physiology</subject><subject>Molecular Sequence Data</subject><subject>Neoplasms - genetics</subject><subject>Oligodeoxyribonucleotides</subject><subject>pleiotrophin</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>role</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFtrFTEQx4Mo9bT6EQr7IGIftiabS5NHKd6goFAF38JszqwbyV7M7FL99s1xj-3kYfgzv7nkz9i54JeCC_OWOG9E7Rpt3wh7IbVxTW2fsJ3gVtZSix9P2e4Bec5OiX7xEsqJE3YilFGGNzsWvyaM05KnuY9jRUsc1gQLUtXFNk9tAlqognFf4biflh5ThLTpOf6XAVPaoEgV_pkzEmERY9WvA4xVgDFgfsGedZAIXx7zGfv-4f2360_1zZePn6_f3dRBc7PUSjeSK82h485wLSQHtEJ1oBw4jp1zDsCEtjyFWqvQGuzklQ1gZWtDkGfs9TZ3ztPvFWnxQ6TDiTDitJIXRjnVOF5AvYEhT0QZOz_nOED-6wX3B4v97cE_f_DPC-v_Wext6Ts_LljbAfePXZunpf7qWAcKkLpcvh_pAVPlaCvVI9bHn_1dzOjbOIUeB9-YKy-NL2utk_fGnJHA</recordid><startdate>19921225</startdate><enddate>19921225</enddate><creator>WENJING FANG</creator><creator>HARTMANN, N</creator><creator>CHOW, D. T</creator><creator>TATE RIEGEL, A</creator><creator>WELLSTEIN, A</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope></search><sort><creationdate>19921225</creationdate><title>Pleiotrophin stimulates fibroblasts and endothelial and epithelial cells and is expressed in human cancer</title><author>WENJING FANG ; HARTMANN, N ; CHOW, D. T ; TATE RIEGEL, A ; WELLSTEIN, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-45230450af09605130ae814fa49a90ef999aa6cbcbc4e554cb6ef378ca83b8cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adrenal Gland Neoplasms</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>carcinoma</topic><topic>Carrier Proteins</topic><topic>Cell Division - drug effects</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>Cytokines - genetics</topic><topic>Cytokines - pharmacology</topic><topic>Cytokines - physiology</topic><topic>embryogenesis</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>expression</topic><topic>Female</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Genetic Vectors</topic><topic>Host-tumor relations. Immunology. Biological markers</topic><topic>Humans</topic><topic>Insecta</topic><topic>Kidney</topic><topic>Male</topic><topic>man</topic><topic>Medical sciences</topic><topic>Mitogens - physiology</topic><topic>Molecular Sequence Data</topic><topic>Neoplasms - genetics</topic><topic>Oligodeoxyribonucleotides</topic><topic>pleiotrophin</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>role</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WENJING FANG</creatorcontrib><creatorcontrib>HARTMANN, N</creatorcontrib><creatorcontrib>CHOW, D. T</creatorcontrib><creatorcontrib>TATE RIEGEL, A</creatorcontrib><creatorcontrib>WELLSTEIN, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WENJING FANG</au><au>HARTMANN, N</au><au>CHOW, D. T</au><au>TATE RIEGEL, A</au><au>WELLSTEIN, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pleiotrophin stimulates fibroblasts and endothelial and epithelial cells and is expressed in human cancer</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1992-12-25</date><risdate>1992</risdate><volume>267</volume><issue>36</issue><spage>25889</spage><epage>25897</epage><pages>25889-25897</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Previously we reported the purification of the heparin-binding growth factor pleiotrophin (PTN) from supernatants of the human
breast cancer cell line MDA-MB-231. To investigate further the biological activities of PTN and its potential role in cancer,
we cloned a PTN cDNA and expressed the gene in a human kidney and in a human adrenal carcinoma cell line (SW-13). The supernatants
harvested from cells transfected with PTN contained a heparin-binding specific protein of an apparent molecular mass of 18
kDa. These supernatants stimulated the proliferation of endothelial cells as well as the anchorage-independent growth of SW-13
cells and of normal rat kidney fibroblasts. Furthermore, SW-13 cells transfected with PTN acquired autonomous growth in soft
agar and were tumorigenic in athymic nude mice. In contrast to these results with PTN from human cells, PTN obtained from
insect cells (Sf9) using recombinant baculovirus as a vector was biologically inactive. We detected high levels of PTN mRNA
in 16 of 27 primary human breast cancer samples (62%) as well as in 8 of 8 carcinogen-induced rat mammary tumors. Furthermore,
9 of 34 human tumor cell lines of different origin showed detectable PTN mRNA. We conclude that PTN may function as a tumor
growth and angiogenesis factor in addition to its role during embryonic development.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1464602</pmid><doi>10.1016/s0021-9258(18)35692-8</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1992-12, Vol.267 (36), p.25889-25897 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16494290 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adrenal Gland Neoplasms Amino Acid Sequence Animals Base Sequence Biological and medical sciences carcinoma Carrier Proteins Cell Division - drug effects Cell Line Cloning, Molecular Cytokines - genetics Cytokines - pharmacology Cytokines - physiology embryogenesis Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Epithelial Cells - cytology Epithelial Cells - drug effects expression Female Fibroblasts - cytology Fibroblasts - drug effects Genetic Vectors Host-tumor relations. Immunology. Biological markers Humans Insecta Kidney Male man Medical sciences Mitogens - physiology Molecular Sequence Data Neoplasms - genetics Oligodeoxyribonucleotides pleiotrophin Polymerase Chain Reaction Promoter Regions, Genetic RNA, Messenger - analysis RNA, Messenger - genetics RNA, Messenger - metabolism role Transfection Tumor Cells, Cultured Tumors |
| title | Pleiotrophin stimulates fibroblasts and endothelial and epithelial cells and is expressed in human cancer |
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