Analysis of Tween 80 as an esterase/ lipase substrate for lipolytic activity assay

The Tween 80 assay to detect lipolytic activities in agar media was evaluated. A spectrophotometric assay for Tween 80 hydrolysis was established. The specific activities with Tween 80, as well as with some conventional lipase-type and esterase-type substrates, were measured using several lipases an...

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Veröffentlicht in:	Biotechnology techniques 1998, Vol.12 (3), p.183-186
Hauptverfasser:	PLOU, F. J, FERRER, M, NUERO, O. M, CALVO, M. V, ALCALDE, M, REYES, F, BALLESTEROS, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Enzyme engineering Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous
Online-Zugang:	Volltext
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