Phenotypic analysis of proteinase a mutants. Implications for autoactivation and the maturation pathway of the vacuolar hydrolases of Saccharomyces cerevisiae

We have isolated a number of mutants deficient in activity of the vacuolar hydrolase proteinase A (PrA). The mutations were sequenced and although they all map in the PEP4 gene, which encodes the precursor to PrA, three distinguishable phenotypes have surfaced. The properties of the pep4-7 missense...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 1993-04, Vol.268 (12), p.8990-8998
Hauptverfasser:	Woolford, C.A, Noble, J.A, Garman, J.D, Tam, M.F, Innis, M.A, Jones, E.W
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Aspartic Acid Endopeptidases - genetics Aspartic Acid Endopeptidases - isolation & purification Aspartic Acid Endopeptidases - metabolism Base Sequence Binding Sites Biological and medical sciences DNA, Fungal Enzyme Activation Enzymes and enzyme inhibitors FENOTIPOS Fundamental and applied biological sciences. Psychology Genetic Complementation Test Hydrolases Hydrolases - metabolism Molecular Sequence Data Mutagenesis, Site-Directed MUTANT MUTANTES PHENOTYPE PROTEASAS PROTEASE Restriction Mapping SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE VACUOLA VACUOLE Vacuoles - enzymology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!