Applicability of a Lactobacillus amylovorus strain as co-culture for natural folate bio-enrichment of fermented milk

The ability of 55 strains from different Lactobacillus species to produce folate was investigated. In order to evaluate folic acid productivity, lactobacilli were cultivated in the folate-free culture medium (FACM). Most of the tested strains needed folate for growth. The production and the extent o...

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Veröffentlicht in:International journal of food microbiology 2014-11, Vol.191, p.10-16
Hauptverfasser: Laiño, Jonathan Emiliano, Juarez del Valle, Marianela, Savoy de Giori, Graciela, LeBlanc, Jean Guy Joseph
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container_title International journal of food microbiology
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creator Laiño, Jonathan Emiliano
Juarez del Valle, Marianela
Savoy de Giori, Graciela
LeBlanc, Jean Guy Joseph
description The ability of 55 strains from different Lactobacillus species to produce folate was investigated. In order to evaluate folic acid productivity, lactobacilli were cultivated in the folate-free culture medium (FACM). Most of the tested strains needed folate for growth. The production and the extent of vitamin accumulation were distinctive features of individual strains. Lactobacillus amylovorus CRL887 was selected for further studies because of its ability to produce significantly higher concentrations of vitamin (81.2±5.4μg/L). The safety of this newly identified folate producing strain was evaluated through healthy experimental mice. No bacterial translocation was detected in liver and spleen after consumption of CRL887 during 7 days and no undesirable side effects were observed in the animals that received this strain. This strain in co-culture with previously selected folate producing starter cultures (Lactobacillus bulgaricus CRL871, and Streptococcus thermophilus CRL803 and CRL415) yielded a yogurt containing high folate concentrations (263.1±2.4μg/L); a single portion of which would provide 15% of the recommended dietary allowance. This is the first report where a Lactobacillus amylovorus strain was successfully used as co-culture for natural folate bio-enrichment of fermented milk. •Lactobacillus amylovorus CRL887 produces high folate concentrations.•Co-culture of L. amylovorus CRL887 increases folate levels in yogurt.•Yogurt by co-culture of L. amylovorus CRL887 provides 14% RDA.
doi_str_mv 10.1016/j.ijfoodmicro.2014.08.031
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In order to evaluate folic acid productivity, lactobacilli were cultivated in the folate-free culture medium (FACM). Most of the tested strains needed folate for growth. The production and the extent of vitamin accumulation were distinctive features of individual strains. Lactobacillus amylovorus CRL887 was selected for further studies because of its ability to produce significantly higher concentrations of vitamin (81.2±5.4μg/L). The safety of this newly identified folate producing strain was evaluated through healthy experimental mice. No bacterial translocation was detected in liver and spleen after consumption of CRL887 during 7 days and no undesirable side effects were observed in the animals that received this strain. This strain in co-culture with previously selected folate producing starter cultures (Lactobacillus bulgaricus CRL871, and Streptococcus thermophilus CRL803 and CRL415) yielded a yogurt containing high folate concentrations (263.1±2.4μg/L); a single portion of which would provide 15% of the recommended dietary allowance. 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Psychology ; Lactic acid bacteria ; Lactobacillus ; Lactobacillus - growth &amp; development ; Lactobacillus - metabolism ; Lactobacillus amylovorus ; Lactobacillus bulgaricus ; Mice ; Milk - microbiology ; Streptococcus thermophilus ; Streptococcus thermophilus - metabolism ; Yogurt - analysis ; Yogurt - microbiology</subject><ispartof>International journal of food microbiology, 2014-11, Vol.191, p.10-16</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. 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In order to evaluate folic acid productivity, lactobacilli were cultivated in the folate-free culture medium (FACM). Most of the tested strains needed folate for growth. The production and the extent of vitamin accumulation were distinctive features of individual strains. Lactobacillus amylovorus CRL887 was selected for further studies because of its ability to produce significantly higher concentrations of vitamin (81.2±5.4μg/L). The safety of this newly identified folate producing strain was evaluated through healthy experimental mice. No bacterial translocation was detected in liver and spleen after consumption of CRL887 during 7 days and no undesirable side effects were observed in the animals that received this strain. 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subjects Animals
Biological and medical sciences
Coculture Techniques
Fermentation
Fermented milk
Folate
Folic Acid - analysis
Folic Acid - biosynthesis
Food industries
Food Microbiology
Fundamental and applied biological sciences. Psychology
Lactic acid bacteria
Lactobacillus
Lactobacillus - growth & development
Lactobacillus - metabolism
Lactobacillus amylovorus
Lactobacillus bulgaricus
Mice
Milk - microbiology
Streptococcus thermophilus
Streptococcus thermophilus - metabolism
Yogurt - analysis
Yogurt - microbiology
title Applicability of a Lactobacillus amylovorus strain as co-culture for natural folate bio-enrichment of fermented milk
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