Haematology, morphology and blood cells characteristics of male and female Siamese fighting fish (Betta splendens)
The aim of the present study was to obtain baseline data on blood cell size, morphology and haematological parameters in Siamese fighting fish (Betta splendens) since there is limited information in the published literature. Blood samples from the caudal vein of apparently healthy Siamese fighting f...
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creator | Motlagh, Somayeh Pourali Zarejabad, Asad Mohammadi Nasrabadi, Rasol Ghorbani Ahmadifar, Ehsan Molaee, Masoud |
description | The aim of the present study was to obtain baseline data on blood cell size, morphology and haematological parameters in Siamese fighting fish
(Betta splendens)
since there is limited information in the published literature. Blood samples from the caudal vein of apparently healthy Siamese fighting fish (male:
n
= 40 and female:
n
= 36) were collected. Haematological values of the blood samples were determined using standard techniques. The morphological features of blood cells were described according to observations made by light microscopy. The various types of blood cells measurement were carried out with the help of a stage and an ocular micrometre at a magnification of ×1,000. Erythrocytes, thrombocytes and four types of leucocytes: lymphocytes, monocytes, heterophils and eosinophils, were distinguished and characterised. The average size of the erythrocyte cell and nucleus was 97.33 and 16.28 μm
2
, respectively. Results showed a positive correlation between erythrocyte size and nucleus size for Siamese fighting fish (
r
= 0.470,
p
|
doi_str_mv | 10.1007/s00580-010-1058-6 |
format | Article |
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(Betta splendens)
since there is limited information in the published literature. Blood samples from the caudal vein of apparently healthy Siamese fighting fish (male:
n
= 40 and female:
n
= 36) were collected. Haematological values of the blood samples were determined using standard techniques. The morphological features of blood cells were described according to observations made by light microscopy. The various types of blood cells measurement were carried out with the help of a stage and an ocular micrometre at a magnification of ×1,000. Erythrocytes, thrombocytes and four types of leucocytes: lymphocytes, monocytes, heterophils and eosinophils, were distinguished and characterised. The average size of the erythrocyte cell and nucleus was 97.33 and 16.28 μm
2
, respectively. Results showed a positive correlation between erythrocyte size and nucleus size for Siamese fighting fish (
r
= 0.470,
p
< 0.01). We also found sex-dependent differences for total white blood cell count, lymphocytes and heterophils in Siamese fighting fish (
p
< 0.05). Statistical analysis revealed that differences in other haematological parameters and blood cell morphology, between male and female fish were not statistically significant (
p
> 0.05).</description><identifier>ISSN: 1618-5641</identifier><identifier>EISSN: 1618-565X</identifier><identifier>DOI: 10.1007/s00580-010-1058-6</identifier><language>eng</language><publisher>London: Springer-Verlag</publisher><subject>Betta splendens ; Hematology ; Medicine ; Medicine & Public Health ; Oncology ; Original Article ; Pathology</subject><ispartof>Comparative clinical pathology, 2012-02, Vol.21 (1), p.15-21</ispartof><rights>Springer-Verlag London Limited 2010</rights><rights>Springer-Verlag London Limited 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2646-aa8ed424996cd176bdbccd74917f56a615b400a37501512a9236ad75a1865c6d3</citedby><cites>FETCH-LOGICAL-c2646-aa8ed424996cd176bdbccd74917f56a615b400a37501512a9236ad75a1865c6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00580-010-1058-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00580-010-1058-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Motlagh, Somayeh Pourali</creatorcontrib><creatorcontrib>Zarejabad, Asad Mohammadi</creatorcontrib><creatorcontrib>Nasrabadi, Rasol Ghorbani</creatorcontrib><creatorcontrib>Ahmadifar, Ehsan</creatorcontrib><creatorcontrib>Molaee, Masoud</creatorcontrib><title>Haematology, morphology and blood cells characteristics of male and female Siamese fighting fish (Betta splendens)</title><title>Comparative clinical pathology</title><addtitle>Comp Clin Pathol</addtitle><description>The aim of the present study was to obtain baseline data on blood cell size, morphology and haematological parameters in Siamese fighting fish
(Betta splendens)
since there is limited information in the published literature. Blood samples from the caudal vein of apparently healthy Siamese fighting fish (male:
n
= 40 and female:
n
= 36) were collected. Haematological values of the blood samples were determined using standard techniques. The morphological features of blood cells were described according to observations made by light microscopy. The various types of blood cells measurement were carried out with the help of a stage and an ocular micrometre at a magnification of ×1,000. Erythrocytes, thrombocytes and four types of leucocytes: lymphocytes, monocytes, heterophils and eosinophils, were distinguished and characterised. The average size of the erythrocyte cell and nucleus was 97.33 and 16.28 μm
2
, respectively. Results showed a positive correlation between erythrocyte size and nucleus size for Siamese fighting fish (
r
= 0.470,
p
< 0.01). We also found sex-dependent differences for total white blood cell count, lymphocytes and heterophils in Siamese fighting fish (
p
< 0.05). Statistical analysis revealed that differences in other haematological parameters and blood cell morphology, between male and female fish were not statistically significant (
p
> 0.05).</description><subject>Betta splendens</subject><subject>Hematology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Pathology</subject><issn>1618-5641</issn><issn>1618-565X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1Lw0AQhoMoWKs_wNuClwpGd5LsbnLU4hcUPKjgLUx3N21Kkq076aH_3m0qIoKneQaed9jZiaJz4NfAubohzkXOYw48hkCxPIhGICGPhRQfhz-cwXF0QrTiHESepqPIP6FtsXeNW2yvWOv8ejkww86weeOcYdo2DTG9RI-6t76mvtbEXMVabOzgVXbA1xpbS5ZV9WLZ190iAC3Z5M72PTJaN7YztqPL0-iowobs2XcdR-8P92_Tp3j28vg8vZ3FOpGZjBFza7IkKwqpDSg5N3OtjcoKUJWQKEHMM84xVSLsAgkWSSrRKIGQS6GlScfRZD937d3nxlJftjXtlsHOug2VIDPFIU1EGtSLP-rKbXwXXlcCL8K3JZlSwYK9pb0j8rYq175u0W-DVO6uUO6vUPKhF3kpQybZZyi43cL635P_C30BkL2JvA</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Motlagh, Somayeh Pourali</creator><creator>Zarejabad, Asad Mohammadi</creator><creator>Nasrabadi, Rasol Ghorbani</creator><creator>Ahmadifar, Ehsan</creator><creator>Molaee, Masoud</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>201202</creationdate><title>Haematology, morphology and blood cells characteristics of male and female Siamese fighting fish (Betta splendens)</title><author>Motlagh, Somayeh Pourali ; Zarejabad, Asad Mohammadi ; Nasrabadi, Rasol Ghorbani ; Ahmadifar, Ehsan ; Molaee, Masoud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2646-aa8ed424996cd176bdbccd74917f56a615b400a37501512a9236ad75a1865c6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Betta splendens</topic><topic>Hematology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Motlagh, Somayeh Pourali</creatorcontrib><creatorcontrib>Zarejabad, Asad Mohammadi</creatorcontrib><creatorcontrib>Nasrabadi, Rasol Ghorbani</creatorcontrib><creatorcontrib>Ahmadifar, Ehsan</creatorcontrib><creatorcontrib>Molaee, Masoud</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Comparative clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Motlagh, Somayeh Pourali</au><au>Zarejabad, Asad Mohammadi</au><au>Nasrabadi, Rasol Ghorbani</au><au>Ahmadifar, Ehsan</au><au>Molaee, Masoud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Haematology, morphology and blood cells characteristics of male and female Siamese fighting fish (Betta splendens)</atitle><jtitle>Comparative clinical pathology</jtitle><stitle>Comp Clin Pathol</stitle><date>2012-02</date><risdate>2012</risdate><volume>21</volume><issue>1</issue><spage>15</spage><epage>21</epage><pages>15-21</pages><issn>1618-5641</issn><eissn>1618-565X</eissn><abstract>The aim of the present study was to obtain baseline data on blood cell size, morphology and haematological parameters in Siamese fighting fish
(Betta splendens)
since there is limited information in the published literature. Blood samples from the caudal vein of apparently healthy Siamese fighting fish (male:
n
= 40 and female:
n
= 36) were collected. Haematological values of the blood samples were determined using standard techniques. The morphological features of blood cells were described according to observations made by light microscopy. The various types of blood cells measurement were carried out with the help of a stage and an ocular micrometre at a magnification of ×1,000. Erythrocytes, thrombocytes and four types of leucocytes: lymphocytes, monocytes, heterophils and eosinophils, were distinguished and characterised. The average size of the erythrocyte cell and nucleus was 97.33 and 16.28 μm
2
, respectively. Results showed a positive correlation between erythrocyte size and nucleus size for Siamese fighting fish (
r
= 0.470,
p
< 0.01). We also found sex-dependent differences for total white blood cell count, lymphocytes and heterophils in Siamese fighting fish (
p
< 0.05). Statistical analysis revealed that differences in other haematological parameters and blood cell morphology, between male and female fish were not statistically significant (
p
> 0.05).</abstract><cop>London</cop><pub>Springer-Verlag</pub><doi>10.1007/s00580-010-1058-6</doi><tpages>7</tpages></addata></record> |
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subjects | Betta splendens Hematology Medicine Medicine & Public Health Oncology Original Article Pathology |
title | Haematology, morphology and blood cells characteristics of male and female Siamese fighting fish (Betta splendens) |
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