Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR)
In eukaryotes, cell morphogenesis is regulated coordinately with the cell cycle. In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (5), p.1086-1091 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | KUME, Kazunori KUBOTA, Shunsuke KOYANO, Takayuki KANAI, Muneyoshi MIZUNUMA, Masaki TODA, Takashi HIRATA, Dai |
| description | In eukaryotes, cell morphogenesis is regulated coordinately with the cell cycle. In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following cytokinesis. Here we show that the conserved leucine-rich repeat protein Lrp1 is required for cell morphogenesis as a newly recognized component of MOR. Lrp1 has 4 leucine-rich repeats in its N-terminus and is a homolog of the budding yeast Sog2, which is a component of the RAM network (regulation of Ace2 activity and cellular morphogenesis). Lrp1 was essential for both cell growth and cell morphogenesis as were the other MOR components. Lrp1 was localized to the SPBs (spindle pole bodies, the yeast equivalent of the animal centrosome) throughout the cell cycle and to the medial ring during cytokinesis. Lrp1 interacted with Nak1 and was important for Orb6 kinase activity. Thus Lrp1 proved to function upstream of Orb6 in cell morphogenesis. |
| doi_str_mv | 10.1271/bbb.130064 |
| format | Article |
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In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following cytokinesis. Here we show that the conserved leucine-rich repeat protein Lrp1 is required for cell morphogenesis as a newly recognized component of MOR. Lrp1 has 4 leucine-rich repeats in its N-terminus and is a homolog of the budding yeast Sog2, which is a component of the RAM network (regulation of Ace2 activity and cellular morphogenesis). Lrp1 was essential for both cell growth and cell morphogenesis as were the other MOR components. Lrp1 was localized to the SPBs (spindle pole bodies, the yeast equivalent of the animal centrosome) throughout the cell cycle and to the medial ring during cytokinesis. Lrp1 interacted with Nak1 and was important for Orb6 kinase activity. Thus Lrp1 proved to function upstream of Orb6 in cell morphogenesis.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.130064</identifier><identifier>PMID: 23649273</identifier><language>eng</language><publisher>England: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Amino Acid Sequence ; Carrier Proteins - metabolism ; Cell Cycle Proteins - metabolism ; cell polarity ; Cell Proliferation ; cell separation ; Cytokinesis ; Intracellular Signaling Peptides and Proteins - chemistry ; Intracellular Signaling Peptides and Proteins - metabolism ; leucine-rich repeat ; Microtubules - metabolism ; Molecular Sequence Data ; morphogenesis Orb6 network (MOR) ; Protein Serine-Threonine Kinases - metabolism ; Protein Transport ; Saccharomyces cerevisiae ; Schizosaccharomyces - cytology ; Schizosaccharomyces - metabolism ; Schizosaccharomyces pombe ; Schizosaccharomyces pombe Proteins - chemistry ; Schizosaccharomyces pombe Proteins - metabolism ; Sequence Homology, Amino Acid ; yeast</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (5), p.1086-1091</ispartof><rights>2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013</rights><rights>Copyright Japan Science and Technology Agency 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-2c2e800dc84f6def5fb5b6f171238ab124529f1a14434ddd51bbb900cd3994aa3</citedby><cites>FETCH-LOGICAL-c624t-2c2e800dc84f6def5fb5b6f171238ab124529f1a14434ddd51bbb900cd3994aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27906,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23649273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KUME, Kazunori</creatorcontrib><creatorcontrib>KUBOTA, Shunsuke</creatorcontrib><creatorcontrib>KOYANO, Takayuki</creatorcontrib><creatorcontrib>KANAI, Muneyoshi</creatorcontrib><creatorcontrib>MIZUNUMA, Masaki</creatorcontrib><creatorcontrib>TODA, Takashi</creatorcontrib><creatorcontrib>HIRATA, Dai</creatorcontrib><title>Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR)</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>In eukaryotes, cell morphogenesis is regulated coordinately with the cell cycle. In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following cytokinesis. Here we show that the conserved leucine-rich repeat protein Lrp1 is required for cell morphogenesis as a newly recognized component of MOR. Lrp1 has 4 leucine-rich repeats in its N-terminus and is a homolog of the budding yeast Sog2, which is a component of the RAM network (regulation of Ace2 activity and cellular morphogenesis). Lrp1 was essential for both cell growth and cell morphogenesis as were the other MOR components. Lrp1 was localized to the SPBs (spindle pole bodies, the yeast equivalent of the animal centrosome) throughout the cell cycle and to the medial ring during cytokinesis. Lrp1 interacted with Nak1 and was important for Orb6 kinase activity. Thus Lrp1 proved to function upstream of Orb6 in cell morphogenesis.</description><subject>Amino Acid Sequence</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>cell polarity</subject><subject>Cell Proliferation</subject><subject>cell separation</subject><subject>Cytokinesis</subject><subject>Intracellular Signaling Peptides and Proteins - chemistry</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>leucine-rich repeat</subject><subject>Microtubules - metabolism</subject><subject>Molecular Sequence Data</subject><subject>morphogenesis Orb6 network (MOR)</subject><subject>Protein Serine-Threonine Kinases - metabolism</subject><subject>Protein Transport</subject><subject>Saccharomyces cerevisiae</subject><subject>Schizosaccharomyces - cytology</subject><subject>Schizosaccharomyces - metabolism</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins - chemistry</subject><subject>Schizosaccharomyces pombe Proteins - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>yeast</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0d9qFDEUBvAgil2rNz6ABLypwtSc_JudS1laLWxdWfTCq5CZOXFTZ5IxmUH6CL61ka1eFEEIBMKPj5zzEfIc2DnwGt60bXsOgjEtH5AVCFlXupH1Q7JiDehqLRWckCc53zBWHhQ8JidcaNnwWqzIz0ufs4-BfkGbZ7rFpfMBq73vDnSPE9qZfkxxRh_oNk1ArzK9yBnD7O1AXUx0g8NAr2OaDvErBsw-U1sO3cRxiqFAGh2dD3jP7FKr6Qecf8T0jZ5d7_avnpJHzg4Zn93dp-Tz5cWnzftqu3t3tXm7rTrN5VzxjuOasb5bS6d7dMq1qtUOauBibVvgUvHGgQUphez7XkFZT8NY14umkdaKU3J2zJ1S_L5gns3oc1emsAHjkg1oWTMADfL_VCglayW5LvTlPXoTlxTKIAZkvWacsbop6vVRdSnmnNCZKfnRplsDzPzu0pTPmmOXBb-4i1zaEfu_9E95Bagj8KE0Mdqyy6E3s70dYnLJhs5nI_4R_AsXn6qH</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>KUME, Kazunori</creator><creator>KUBOTA, Shunsuke</creator><creator>KOYANO, Takayuki</creator><creator>KANAI, Muneyoshi</creator><creator>MIZUNUMA, Masaki</creator><creator>TODA, Takashi</creator><creator>HIRATA, Dai</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>2013</creationdate><title>Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR)</title><author>KUME, Kazunori ; KUBOTA, Shunsuke ; KOYANO, Takayuki ; KANAI, Muneyoshi ; MIZUNUMA, Masaki ; TODA, Takashi ; HIRATA, Dai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c624t-2c2e800dc84f6def5fb5b6f171238ab124529f1a14434ddd51bbb900cd3994aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino Acid Sequence</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>cell polarity</topic><topic>Cell Proliferation</topic><topic>cell separation</topic><topic>Cytokinesis</topic><topic>Intracellular Signaling Peptides and Proteins - chemistry</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>leucine-rich repeat</topic><topic>Microtubules - metabolism</topic><topic>Molecular Sequence Data</topic><topic>morphogenesis Orb6 network (MOR)</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>Protein Transport</topic><topic>Saccharomyces cerevisiae</topic><topic>Schizosaccharomyces - cytology</topic><topic>Schizosaccharomyces - metabolism</topic><topic>Schizosaccharomyces pombe</topic><topic>Schizosaccharomyces pombe Proteins - chemistry</topic><topic>Schizosaccharomyces pombe Proteins - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KUME, Kazunori</creatorcontrib><creatorcontrib>KUBOTA, Shunsuke</creatorcontrib><creatorcontrib>KOYANO, Takayuki</creatorcontrib><creatorcontrib>KANAI, Muneyoshi</creatorcontrib><creatorcontrib>MIZUNUMA, Masaki</creatorcontrib><creatorcontrib>TODA, Takashi</creatorcontrib><creatorcontrib>HIRATA, Dai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KUME, Kazunori</au><au>KUBOTA, Shunsuke</au><au>KOYANO, Takayuki</au><au>KANAI, Muneyoshi</au><au>MIZUNUMA, Masaki</au><au>TODA, Takashi</au><au>HIRATA, Dai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR)</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2013</date><risdate>2013</risdate><volume>77</volume><issue>5</issue><spage>1086</spage><epage>1091</epage><pages>1086-1091</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>In eukaryotes, cell morphogenesis is regulated coordinately with the cell cycle. In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following cytokinesis. Here we show that the conserved leucine-rich repeat protein Lrp1 is required for cell morphogenesis as a newly recognized component of MOR. Lrp1 has 4 leucine-rich repeats in its N-terminus and is a homolog of the budding yeast Sog2, which is a component of the RAM network (regulation of Ace2 activity and cellular morphogenesis). Lrp1 was essential for both cell growth and cell morphogenesis as were the other MOR components. Lrp1 was localized to the SPBs (spindle pole bodies, the yeast equivalent of the animal centrosome) throughout the cell cycle and to the medial ring during cytokinesis. Lrp1 interacted with Nak1 and was important for Orb6 kinase activity. Thus Lrp1 proved to function upstream of Orb6 in cell morphogenesis.</abstract><cop>England</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>23649273</pmid><doi>10.1271/bbb.130064</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (5), p.1086-1091 |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Carrier Proteins - metabolism Cell Cycle Proteins - metabolism cell polarity Cell Proliferation cell separation Cytokinesis Intracellular Signaling Peptides and Proteins - chemistry Intracellular Signaling Peptides and Proteins - metabolism leucine-rich repeat Microtubules - metabolism Molecular Sequence Data morphogenesis Orb6 network (MOR) Protein Serine-Threonine Kinases - metabolism Protein Transport Saccharomyces cerevisiae Schizosaccharomyces - cytology Schizosaccharomyces - metabolism Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins - chemistry Schizosaccharomyces pombe Proteins - metabolism Sequence Homology, Amino Acid yeast |
| title | Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR) |
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