Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori
Juvenile hormone (JH) I, II and III in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs wa...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (5), p.988-991 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | FURUTA, Kenjiro ICHIKAWA, Akio MURATA, Mika KUWANO, Eiichi SHINODA, Tetsuro SHIOTSUKI, Takahiro |
| description | Juvenile hormone (JH) I, II and III in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 µM sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation. |
| doi_str_mv | 10.1271/bbb.120883 |
| format | Article |
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JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 µM sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.120883</identifier><identifier>PMID: 23649254</identifier><language>eng</language><publisher>England: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Animals ; Biochemistry ; Biotechnology ; Bombyx - chemistry ; Bombyx mori ; Chromatography, Liquid ; Hemolymph - chemistry ; Hormones ; Hydrogen-Ion Concentration ; juvenile hormone ; Juvenile Hormones - analysis ; Juvenile Hormones - chemistry ; liquid chromatography-mass spectrometry (LC-MS) ; Liquids ; Mass Spectrometry ; Methanol - chemistry ; Methyl alcohol ; Salts - chemistry ; silkworm ; Silkworms ; Sodium ; Spectrometry ; Stadiums ; titration</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (5), p.988-991</ispartof><rights>2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013</rights><rights>Copyright Japan Science and Technology Agency 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c727t-665ea75165ff9c018e5e19fb7f61535e8acbb3e6ddfc049236dfd7c5796b3c283</citedby><cites>FETCH-LOGICAL-c727t-665ea75165ff9c018e5e19fb7f61535e8acbb3e6ddfc049236dfd7c5796b3c283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23649254$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FURUTA, Kenjiro</creatorcontrib><creatorcontrib>ICHIKAWA, Akio</creatorcontrib><creatorcontrib>MURATA, Mika</creatorcontrib><creatorcontrib>KUWANO, Eiichi</creatorcontrib><creatorcontrib>SHINODA, Tetsuro</creatorcontrib><creatorcontrib>SHIOTSUKI, Takahiro</creatorcontrib><title>Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Juvenile hormone (JH) I, II and III in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 µM sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Bombyx - chemistry</subject><subject>Bombyx mori</subject><subject>Chromatography, Liquid</subject><subject>Hemolymph - chemistry</subject><subject>Hormones</subject><subject>Hydrogen-Ion Concentration</subject><subject>juvenile hormone</subject><subject>Juvenile Hormones - analysis</subject><subject>Juvenile Hormones - chemistry</subject><subject>liquid chromatography-mass spectrometry (LC-MS)</subject><subject>Liquids</subject><subject>Mass Spectrometry</subject><subject>Methanol - chemistry</subject><subject>Methyl alcohol</subject><subject>Salts - chemistry</subject><subject>silkworm</subject><subject>Silkworms</subject><subject>Sodium</subject><subject>Spectrometry</subject><subject>Stadiums</subject><subject>titration</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U9vFCEYBnBiNHZbvfgBDIkX03QUhuHPHHXVrmaNh9aTBwIMpNQBtjBjnW8vm209GBM98Yb88gaeB4BnGL3CLcevtdZ1QEKQB2CFSccb1nf8IVihHrNGdBQfgeNSrhGqFxQ_BkctYV3f0m4Fvr2zk83BRzX5FKFe4HbdfL6AycFP8w8b_WjhJuWQooWXvtICfYQbG9K4hN3V3k1XFl748fttZWfwbQp6-QlDyv4JeOTUWOzTu_MEfP3w_nK9abZfzj-u32wbw1s-NYxRqzjFjDrXG4SFpRb3TnPHMCXUCmW0JpYNgzOoPpuwwQ3cUN4zTUwryAl4edi7y-lmtmWSwRdjx1FFm-YiMWMIMdoK_B-04zUl0fN_U0Jpx6lAe_riD3qd5hzrnyXuuEAtQgJVdXpQJqdSsnVyl31QeZEYyX2RshYpD0VW_Pxu5ayDHX7T--YqoAfgo6vBq5r-OMhJLWPKLqtofJHkL4t_AUvDqII</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>FURUTA, Kenjiro</creator><creator>ICHIKAWA, Akio</creator><creator>MURATA, Mika</creator><creator>KUWANO, Eiichi</creator><creator>SHINODA, Tetsuro</creator><creator>SHIOTSUKI, Takahiro</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7TB</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>2013</creationdate><title>Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori</title><author>FURUTA, Kenjiro ; ICHIKAWA, Akio ; MURATA, Mika ; KUWANO, Eiichi ; SHINODA, Tetsuro ; SHIOTSUKI, Takahiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c727t-665ea75165ff9c018e5e19fb7f61535e8acbb3e6ddfc049236dfd7c5796b3c283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Bombyx - chemistry</topic><topic>Bombyx mori</topic><topic>Chromatography, Liquid</topic><topic>Hemolymph - chemistry</topic><topic>Hormones</topic><topic>Hydrogen-Ion Concentration</topic><topic>juvenile hormone</topic><topic>Juvenile Hormones - analysis</topic><topic>Juvenile Hormones - chemistry</topic><topic>liquid chromatography-mass spectrometry (LC-MS)</topic><topic>Liquids</topic><topic>Mass Spectrometry</topic><topic>Methanol - chemistry</topic><topic>Methyl alcohol</topic><topic>Salts - chemistry</topic><topic>silkworm</topic><topic>Silkworms</topic><topic>Sodium</topic><topic>Spectrometry</topic><topic>Stadiums</topic><topic>titration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FURUTA, Kenjiro</creatorcontrib><creatorcontrib>ICHIKAWA, Akio</creatorcontrib><creatorcontrib>MURATA, Mika</creatorcontrib><creatorcontrib>KUWANO, Eiichi</creatorcontrib><creatorcontrib>SHINODA, Tetsuro</creatorcontrib><creatorcontrib>SHIOTSUKI, Takahiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FURUTA, Kenjiro</au><au>ICHIKAWA, Akio</au><au>MURATA, Mika</au><au>KUWANO, Eiichi</au><au>SHINODA, Tetsuro</au><au>SHIOTSUKI, Takahiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2013</date><risdate>2013</risdate><volume>77</volume><issue>5</issue><spage>988</spage><epage>991</epage><pages>988-991</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Juvenile hormone (JH) I, II and III in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 µM sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation.</abstract><cop>England</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>23649254</pmid><doi>10.1271/bbb.120883</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Biochemistry Biotechnology Bombyx - chemistry Bombyx mori Chromatography, Liquid Hemolymph - chemistry Hormones Hydrogen-Ion Concentration juvenile hormone Juvenile Hormones - analysis Juvenile Hormones - chemistry liquid chromatography-mass spectrometry (LC-MS) Liquids Mass Spectrometry Methanol - chemistry Methyl alcohol Salts - chemistry silkworm Silkworms Sodium Spectrometry Stadiums titration |
| title | Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori |
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