Acute effects of cadmium on osmoregulation of the freshwater teleost Prochilodus lineatus: Enzymes activity and plasma ions
•Cd effects were evaluated on plasma ions and ATPases of Prochilodus lineatus.•Fish were exposed to 1 and 10μgCdL−1 for 24 and 96h.•Na+/K+-ATPase and carbonic anhydrase decreased in gills and kidney after Cd exposure.•Gill Ca2+-ATPase and plasma Ca2+ decreased after Cd exposure.•Cd concentrations se...
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| Veröffentlicht in: | Aquatic toxicology 2014-11, Vol.156, p.161-168 |
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| description | •Cd effects were evaluated on plasma ions and ATPases of Prochilodus lineatus.•Fish were exposed to 1 and 10μgCdL−1 for 24 and 96h.•Na+/K+-ATPase and carbonic anhydrase decreased in gills and kidney after Cd exposure.•Gill Ca2+-ATPase and plasma Ca2+ decreased after Cd exposure.•Cd concentrations set by Brazilian guidelines affect Ca2+ regulation of P. lineatus.
Cadmium (Cd) is a trace element that is very toxic to fish. It is commonly found in surface waters contaminated with industrial effluents. When dissolved in water, Cd can rapidly cause physiological changes in the gills and kidneys of freshwater fish. The objective of this study was to evaluate the acute effects of Cd on the osmoregulation of the Neotropical fish Prochilodus lineatus. Juvenile fish were exposed to Cd at two concentrations [1 (Cd1) and 10 (Cd10) μgL−1] for 24 and 96h. The effects of Cd were evaluated through the analysis of ions (Na+, K+, Ca2+, and Cl−) and plasma osmolality, and by measuring the activities of enzymes involved in osmoregulation obtained from the gills and kidney. Fish exposed to Cd for 24 and 96h showed a decrease in Na+/K+-ATPase activity in the gills and kidney. The activity of carbonic anhydrase decreased in the gills after 24h and in both tissues after 96h of Cd exposure. The gill Ca2+-ATPase activity also decreased with Cd exposure, with a concomitant drop in the plasma concentration of Ca2+. Despite the hypocalcemia, there were no changes in the concentration of the ions Na+, K+, and Cl− or in plasma osmolality. Among the enzymes involved in ion transport, H+-ATPase was the only enzyme that showed increased activity in gills, whereas its activity in kidney remained unchanged. The results of this study demonstrate that waterborne Cd at the maximum concentrations set by Brazilian guidelines for freshwater affects the gills and kidney functions of P. lineatus. Acute exposure to Cd resulted in the decrease of the activity of enzymes, which culminated with the loss of the fish's ability to regulate the levels of calcium in the blood, leading to hypocalcemia. |
| doi_str_mv | 10.1016/j.aquatox.2014.08.009 |
| format | Article |
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Cadmium (Cd) is a trace element that is very toxic to fish. It is commonly found in surface waters contaminated with industrial effluents. When dissolved in water, Cd can rapidly cause physiological changes in the gills and kidneys of freshwater fish. The objective of this study was to evaluate the acute effects of Cd on the osmoregulation of the Neotropical fish Prochilodus lineatus. Juvenile fish were exposed to Cd at two concentrations [1 (Cd1) and 10 (Cd10) μgL−1] for 24 and 96h. The effects of Cd were evaluated through the analysis of ions (Na+, K+, Ca2+, and Cl−) and plasma osmolality, and by measuring the activities of enzymes involved in osmoregulation obtained from the gills and kidney. Fish exposed to Cd for 24 and 96h showed a decrease in Na+/K+-ATPase activity in the gills and kidney. The activity of carbonic anhydrase decreased in the gills after 24h and in both tissues after 96h of Cd exposure. The gill Ca2+-ATPase activity also decreased with Cd exposure, with a concomitant drop in the plasma concentration of Ca2+. Despite the hypocalcemia, there were no changes in the concentration of the ions Na+, K+, and Cl− or in plasma osmolality. Among the enzymes involved in ion transport, H+-ATPase was the only enzyme that showed increased activity in gills, whereas its activity in kidney remained unchanged. The results of this study demonstrate that waterborne Cd at the maximum concentrations set by Brazilian guidelines for freshwater affects the gills and kidney functions of P. lineatus. Acute exposure to Cd resulted in the decrease of the activity of enzymes, which culminated with the loss of the fish's ability to regulate the levels of calcium in the blood, leading to hypocalcemia.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2014.08.009</identifier><identifier>PMID: 25203423</identifier><identifier>CODEN: AQTODG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agnatha. Pisces ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; Brazil ; Ca2+-ATPase ; Cadmium - toxicity ; Carbonic anhydrase ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on vertebrates ; Enzyme Activation - drug effects ; Fishes - metabolism ; Fresh Water ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gills - drug effects ; Gills - enzymology ; H+-ATPase ; Hypocalcemia ; Ion Transport - drug effects ; Ions - blood ; Ions - metabolism ; Kidney - drug effects ; Na+/K+-ATPase ; Osmoregulation - drug effects ; Prochilodus lineatus ; Sodium-Potassium-Exchanging ATPase - metabolism ; Teleostei ; Water Pollutants, Chemical - toxicity</subject><ispartof>Aquatic toxicology, 2014-11, Vol.156, p.161-168</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-a66147af0000be37515fd33d4e6abc32402a45a128efb29d5ea97e1573c0f1ca3</citedby><cites>FETCH-LOGICAL-c428t-a66147af0000be37515fd33d4e6abc32402a45a128efb29d5ea97e1573c0f1ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquatox.2014.08.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28887744$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25203423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, Alexandre O.F. da</creatorcontrib><creatorcontrib>Martinez, Cláudia B.R.</creatorcontrib><title>Acute effects of cadmium on osmoregulation of the freshwater teleost Prochilodus lineatus: Enzymes activity and plasma ions</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>•Cd effects were evaluated on plasma ions and ATPases of Prochilodus lineatus.•Fish were exposed to 1 and 10μgCdL−1 for 24 and 96h.•Na+/K+-ATPase and carbonic anhydrase decreased in gills and kidney after Cd exposure.•Gill Ca2+-ATPase and plasma Ca2+ decreased after Cd exposure.•Cd concentrations set by Brazilian guidelines affect Ca2+ regulation of P. lineatus.
Cadmium (Cd) is a trace element that is very toxic to fish. It is commonly found in surface waters contaminated with industrial effluents. When dissolved in water, Cd can rapidly cause physiological changes in the gills and kidneys of freshwater fish. The objective of this study was to evaluate the acute effects of Cd on the osmoregulation of the Neotropical fish Prochilodus lineatus. Juvenile fish were exposed to Cd at two concentrations [1 (Cd1) and 10 (Cd10) μgL−1] for 24 and 96h. The effects of Cd were evaluated through the analysis of ions (Na+, K+, Ca2+, and Cl−) and plasma osmolality, and by measuring the activities of enzymes involved in osmoregulation obtained from the gills and kidney. Fish exposed to Cd for 24 and 96h showed a decrease in Na+/K+-ATPase activity in the gills and kidney. The activity of carbonic anhydrase decreased in the gills after 24h and in both tissues after 96h of Cd exposure. The gill Ca2+-ATPase activity also decreased with Cd exposure, with a concomitant drop in the plasma concentration of Ca2+. Despite the hypocalcemia, there were no changes in the concentration of the ions Na+, K+, and Cl− or in plasma osmolality. Among the enzymes involved in ion transport, H+-ATPase was the only enzyme that showed increased activity in gills, whereas its activity in kidney remained unchanged. The results of this study demonstrate that waterborne Cd at the maximum concentrations set by Brazilian guidelines for freshwater affects the gills and kidney functions of P. lineatus. Acute exposure to Cd resulted in the decrease of the activity of enzymes, which culminated with the loss of the fish's ability to regulate the levels of calcium in the blood, leading to hypocalcemia.</description><subject>Agnatha. Pisces</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Brazil</subject><subject>Ca2+-ATPase</subject><subject>Cadmium - toxicity</subject><subject>Carbonic anhydrase</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on vertebrates</subject><subject>Enzyme Activation - drug effects</subject><subject>Fishes - metabolism</subject><subject>Fresh Water</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gills - drug effects</subject><subject>Gills - enzymology</subject><subject>H+-ATPase</subject><subject>Hypocalcemia</subject><subject>Ion Transport - drug effects</subject><subject>Ions - blood</subject><subject>Ions - metabolism</subject><subject>Kidney - drug effects</subject><subject>Na+/K+-ATPase</subject><subject>Osmoregulation - drug effects</subject><subject>Prochilodus lineatus</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Teleostei</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuLFDEQgIMo7rj6E5RcBC89JumkH15kWdYHLOhBwVuoSVecDN2d2VR63XH_vN3MqEetS1Hw1YP6GHsuxVoKWb3ereFmghzv1kpIvRbNWoj2AVvJpm4LaaR-yFYzVxVam29n7AnRTsyhdPuYnSmjRKlVuWL3F27KyNF7dJl49NxBN4Rp4HHkkYaY8PvUQw5L6XneIvcJafsDMiaescdImX9O0W1DH7uJeB9GhDzRG341_jwMSBxcDrchHziMHd_3QAPweR49ZY889ITPTvmcfX139eXyQ3H96f3Hy4vrwmnV5AKqSuoa_HL-BsvaSOO7suw0VrBxpdJCgTYgVYN-o9rOILQ1SlOXTnjpoDxnr45z9yneTEjZDoEc9j2MGCeystK1EJVqxX-gqm1N1ZgFNUfUpUiU0Nt9CgOkg5XCLorszp4U2UWRFY2dFc19L04rps2A3Z-u305m4OUJAHLQ-wSjC_SXa5qmrrWeubdHDuff3QZMllzA0WEX0izTdjH845RftIy0NA</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Silva, Alexandre O.F. da</creator><creator>Martinez, Cláudia B.R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QH</scope><scope>7ST</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope></search><sort><creationdate>20141101</creationdate><title>Acute effects of cadmium on osmoregulation of the freshwater teleost Prochilodus lineatus: Enzymes activity and plasma ions</title><author>Silva, Alexandre O.F. da ; Martinez, Cláudia B.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-a66147af0000be37515fd33d4e6abc32402a45a128efb29d5ea97e1573c0f1ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Agnatha. Pisces</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Brazil</topic><topic>Ca2+-ATPase</topic><topic>Cadmium - toxicity</topic><topic>Carbonic anhydrase</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on vertebrates</topic><topic>Enzyme Activation - drug effects</topic><topic>Fishes - metabolism</topic><topic>Fresh Water</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gills - drug effects</topic><topic>Gills - enzymology</topic><topic>H+-ATPase</topic><topic>Hypocalcemia</topic><topic>Ion Transport - drug effects</topic><topic>Ions - blood</topic><topic>Ions - metabolism</topic><topic>Kidney - drug effects</topic><topic>Na+/K+-ATPase</topic><topic>Osmoregulation - drug effects</topic><topic>Prochilodus lineatus</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>Teleostei</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva, Alexandre O.F. da</creatorcontrib><creatorcontrib>Martinez, Cláudia B.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, Alexandre O.F. da</au><au>Martinez, Cláudia B.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute effects of cadmium on osmoregulation of the freshwater teleost Prochilodus lineatus: Enzymes activity and plasma ions</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>156</volume><spage>161</spage><epage>168</epage><pages>161-168</pages><issn>0166-445X</issn><eissn>1879-1514</eissn><coden>AQTODG</coden><abstract>•Cd effects were evaluated on plasma ions and ATPases of Prochilodus lineatus.•Fish were exposed to 1 and 10μgCdL−1 for 24 and 96h.•Na+/K+-ATPase and carbonic anhydrase decreased in gills and kidney after Cd exposure.•Gill Ca2+-ATPase and plasma Ca2+ decreased after Cd exposure.•Cd concentrations set by Brazilian guidelines affect Ca2+ regulation of P. lineatus.
Cadmium (Cd) is a trace element that is very toxic to fish. It is commonly found in surface waters contaminated with industrial effluents. When dissolved in water, Cd can rapidly cause physiological changes in the gills and kidneys of freshwater fish. The objective of this study was to evaluate the acute effects of Cd on the osmoregulation of the Neotropical fish Prochilodus lineatus. Juvenile fish were exposed to Cd at two concentrations [1 (Cd1) and 10 (Cd10) μgL−1] for 24 and 96h. The effects of Cd were evaluated through the analysis of ions (Na+, K+, Ca2+, and Cl−) and plasma osmolality, and by measuring the activities of enzymes involved in osmoregulation obtained from the gills and kidney. Fish exposed to Cd for 24 and 96h showed a decrease in Na+/K+-ATPase activity in the gills and kidney. The activity of carbonic anhydrase decreased in the gills after 24h and in both tissues after 96h of Cd exposure. The gill Ca2+-ATPase activity also decreased with Cd exposure, with a concomitant drop in the plasma concentration of Ca2+. Despite the hypocalcemia, there were no changes in the concentration of the ions Na+, K+, and Cl− or in plasma osmolality. Among the enzymes involved in ion transport, H+-ATPase was the only enzyme that showed increased activity in gills, whereas its activity in kidney remained unchanged. The results of this study demonstrate that waterborne Cd at the maximum concentrations set by Brazilian guidelines for freshwater affects the gills and kidney functions of P. lineatus. Acute exposure to Cd resulted in the decrease of the activity of enzymes, which culminated with the loss of the fish's ability to regulate the levels of calcium in the blood, leading to hypocalcemia.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>25203423</pmid><doi>10.1016/j.aquatox.2014.08.009</doi><tpages>8</tpages></addata></record> |
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| subjects | Agnatha. Pisces Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Brazil Ca2+-ATPase Cadmium - toxicity Carbonic anhydrase Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates Enzyme Activation - drug effects Fishes - metabolism Fresh Water Freshwater Fundamental and applied biological sciences. Psychology Gills - drug effects Gills - enzymology H+-ATPase Hypocalcemia Ion Transport - drug effects Ions - blood Ions - metabolism Kidney - drug effects Na+/K+-ATPase Osmoregulation - drug effects Prochilodus lineatus Sodium-Potassium-Exchanging ATPase - metabolism Teleostei Water Pollutants, Chemical - toxicity |
| title | Acute effects of cadmium on osmoregulation of the freshwater teleost Prochilodus lineatus: Enzymes activity and plasma ions |
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