Aire-dependent peripheral tissue antigen mRNAs in mTEC cells feature networking refractoriness to microRNA interaction
Abstract The downregulation of PTA genes in mTECs is associated with the loss of self-tolerance, and the role of miRNAs in this process is not fully understood. Therefore, we studied the expression of mRNAs and miRNAs in mTECs from autoimmune NOD mice during the period when loss of self-tolerance oc...
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| Veröffentlicht in: | Immunobiology (1979) 2015-01, Vol.220 (1), p.93-102 |
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| container_title | Immunobiology (1979) |
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| creator | Macedo, Claudia Oliveira, Ernna H Almeida, Renata S Donate, Paula B Fornari, Thaís A Pezzi, Nicole Sakamoto-Hojo, Elza T Donadi, Eduardo A Passos, Geraldo A |
| description | Abstract The downregulation of PTA genes in mTECs is associated with the loss of self-tolerance, and the role of miRNAs in this process is not fully understood. Therefore, we studied the expression of mRNAs and miRNAs in mTECs from autoimmune NOD mice during the period when loss of self-tolerance occurs in parallel with non-autoimmune BALB/c mice. Although the expression of the transcriptional regulator Aire was unchanged, we observed downregulation of a set of PTA mRNAs. A set of miRNAs was also differentially expressed in these mice. The reconstruction of miRNA–mRNA interaction networks identified the controller miRNAs and predicted the PTA mRNA targets. Interestingly, the known Aire-dependent PTAs exhibited pronounced refractoriness in the networking interaction with miRNAs. This study reveals the existence of a new mechanism in mTECs, and this mechanism may have importance in the control of self-tolerance. |
| doi_str_mv | 10.1016/j.imbio.2014.08.015 |
| format | Article |
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Therefore, we studied the expression of mRNAs and miRNAs in mTECs from autoimmune NOD mice during the period when loss of self-tolerance occurs in parallel with non-autoimmune BALB/c mice. Although the expression of the transcriptional regulator Aire was unchanged, we observed downregulation of a set of PTA mRNAs. A set of miRNAs was also differentially expressed in these mice. The reconstruction of miRNA–mRNA interaction networks identified the controller miRNAs and predicted the PTA mRNA targets. Interestingly, the known Aire-dependent PTAs exhibited pronounced refractoriness in the networking interaction with miRNAs. This study reveals the existence of a new mechanism in mTECs, and this mechanism may have importance in the control of self-tolerance.</description><identifier>ISSN: 0171-2985</identifier><identifier>EISSN: 1878-3279</identifier><identifier>DOI: 10.1016/j.imbio.2014.08.015</identifier><identifier>PMID: 25220732</identifier><language>eng</language><publisher>Netherlands: Elsevier GmbH</publisher><subject>Advanced Basic Science ; Aire gene ; AIRE Protein ; Allergy and Immunology ; Animals ; Animals, Newborn ; Antigens - genetics ; Antigens - immunology ; Cluster Analysis ; Epistasis, Genetic ; Epithelial Cells - immunology ; Epithelial Cells - metabolism ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Gene Regulatory Networks ; Mice ; microRNAs ; MicroRNAs - genetics ; Peripheral tissue antigen ; Post-transcriptional control ; RNA Interference ; RNA, Messenger - genetics ; Self Tolerance ; Thymus ; Thymus Gland - cytology ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic</subject><ispartof>Immunobiology (1979), 2015-01, Vol.220 (1), p.93-102</ispartof><rights>Elsevier GmbH</rights><rights>2014 Elsevier GmbH</rights><rights>Copyright © 2014 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-7211c189ea75e3018d6a7343ad4a4b88343e421677676f12ed23b1d41819a68d3</citedby><cites>FETCH-LOGICAL-c517t-7211c189ea75e3018d6a7343ad4a4b88343e421677676f12ed23b1d41819a68d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0171298514001582$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25220732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Macedo, Claudia</creatorcontrib><creatorcontrib>Oliveira, Ernna H</creatorcontrib><creatorcontrib>Almeida, Renata S</creatorcontrib><creatorcontrib>Donate, Paula B</creatorcontrib><creatorcontrib>Fornari, Thaís A</creatorcontrib><creatorcontrib>Pezzi, Nicole</creatorcontrib><creatorcontrib>Sakamoto-Hojo, Elza T</creatorcontrib><creatorcontrib>Donadi, Eduardo A</creatorcontrib><creatorcontrib>Passos, Geraldo A</creatorcontrib><title>Aire-dependent peripheral tissue antigen mRNAs in mTEC cells feature networking refractoriness to microRNA interaction</title><title>Immunobiology (1979)</title><addtitle>Immunobiology</addtitle><description>Abstract The downregulation of PTA genes in mTECs is associated with the loss of self-tolerance, and the role of miRNAs in this process is not fully understood. Therefore, we studied the expression of mRNAs and miRNAs in mTECs from autoimmune NOD mice during the period when loss of self-tolerance occurs in parallel with non-autoimmune BALB/c mice. Although the expression of the transcriptional regulator Aire was unchanged, we observed downregulation of a set of PTA mRNAs. A set of miRNAs was also differentially expressed in these mice. The reconstruction of miRNA–mRNA interaction networks identified the controller miRNAs and predicted the PTA mRNA targets. Interestingly, the known Aire-dependent PTAs exhibited pronounced refractoriness in the networking interaction with miRNAs. This study reveals the existence of a new mechanism in mTECs, and this mechanism may have importance in the control of self-tolerance.</description><subject>Advanced Basic Science</subject><subject>Aire gene</subject><subject>AIRE Protein</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antigens - genetics</subject><subject>Antigens - immunology</subject><subject>Cluster Analysis</subject><subject>Epistasis, Genetic</subject><subject>Epithelial Cells - immunology</subject><subject>Epithelial Cells - metabolism</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Gene Regulatory Networks</subject><subject>Mice</subject><subject>microRNAs</subject><subject>MicroRNAs - genetics</subject><subject>Peripheral tissue antigen</subject><subject>Post-transcriptional control</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>Self Tolerance</subject><subject>Thymus</subject><subject>Thymus Gland - cytology</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><issn>0171-2985</issn><issn>1878-3279</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk9v1DAQxS0EokvhEyAhH7kkeOwkdg4grVblj1SBBOVsee3Z4m3iBNsp6rfHYQsHLnDySH7P45nfI-Q5sBoYdK-OtR_3fqo5g6ZmqmbQPiAbUFJVgsv-IdkwkFDxXrVn5ElKR8ag51I9Jme85ZxJwTfkdusjVg5nDA5DpjNGP3_DaAaafUoLUhOyv8ZAx88ft4n6Ulxd7KjFYUj0gCYvEWnA_GOKNz5c04iHaGyeog-YEs0THb2NUzEXb8b1zk_hKXl0MEPCZ_fnOfn69uJq9766_PTuw257WdkWZK4kB7CgejSyRcFAuc5I0QjjGtPslSolNhw6KTvZHYCj42IPrgEFvemUE-fk5endOU7fF0xZjz6tfzcBpyVp6BrJWCuY-A8p73vZCN4UqThJy2AplYn1HP1o4p0Gplc2-qh_sdErG82ULmyK68V9g2U_ovvj-Q2jCF6fBFg2cusx6mQ9BouuMLJZu8n_o8Gbv_x28MFbM9zgHabjtMRQlq1BJ66Z_rLGY00HNCUZreLiJ7-JtZY</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Macedo, Claudia</creator><creator>Oliveira, Ernna H</creator><creator>Almeida, Renata S</creator><creator>Donate, Paula B</creator><creator>Fornari, Thaís A</creator><creator>Pezzi, Nicole</creator><creator>Sakamoto-Hojo, Elza T</creator><creator>Donadi, Eduardo A</creator><creator>Passos, Geraldo A</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20150101</creationdate><title>Aire-dependent peripheral tissue antigen mRNAs in mTEC cells feature networking refractoriness to microRNA interaction</title><author>Macedo, Claudia ; Oliveira, Ernna H ; Almeida, Renata S ; Donate, Paula B ; Fornari, Thaís A ; Pezzi, Nicole ; Sakamoto-Hojo, Elza T ; Donadi, Eduardo A ; Passos, Geraldo A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-7211c189ea75e3018d6a7343ad4a4b88343e421677676f12ed23b1d41819a68d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Advanced Basic Science</topic><topic>Aire gene</topic><topic>AIRE Protein</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antigens - genetics</topic><topic>Antigens - immunology</topic><topic>Cluster Analysis</topic><topic>Epistasis, Genetic</topic><topic>Epithelial Cells - immunology</topic><topic>Epithelial Cells - metabolism</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Gene Regulatory Networks</topic><topic>Mice</topic><topic>microRNAs</topic><topic>MicroRNAs - genetics</topic><topic>Peripheral tissue antigen</topic><topic>Post-transcriptional control</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>Self Tolerance</topic><topic>Thymus</topic><topic>Thymus Gland - cytology</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Macedo, Claudia</creatorcontrib><creatorcontrib>Oliveira, Ernna H</creatorcontrib><creatorcontrib>Almeida, Renata S</creatorcontrib><creatorcontrib>Donate, Paula B</creatorcontrib><creatorcontrib>Fornari, Thaís A</creatorcontrib><creatorcontrib>Pezzi, Nicole</creatorcontrib><creatorcontrib>Sakamoto-Hojo, Elza T</creatorcontrib><creatorcontrib>Donadi, Eduardo A</creatorcontrib><creatorcontrib>Passos, Geraldo A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Immunobiology (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Macedo, Claudia</au><au>Oliveira, Ernna H</au><au>Almeida, Renata S</au><au>Donate, Paula B</au><au>Fornari, Thaís A</au><au>Pezzi, Nicole</au><au>Sakamoto-Hojo, Elza T</au><au>Donadi, Eduardo A</au><au>Passos, Geraldo A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aire-dependent peripheral tissue antigen mRNAs in mTEC cells feature networking refractoriness to microRNA interaction</atitle><jtitle>Immunobiology (1979)</jtitle><addtitle>Immunobiology</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>220</volume><issue>1</issue><spage>93</spage><epage>102</epage><pages>93-102</pages><issn>0171-2985</issn><eissn>1878-3279</eissn><abstract>Abstract The downregulation of PTA genes in mTECs is associated with the loss of self-tolerance, and the role of miRNAs in this process is not fully understood. Therefore, we studied the expression of mRNAs and miRNAs in mTECs from autoimmune NOD mice during the period when loss of self-tolerance occurs in parallel with non-autoimmune BALB/c mice. Although the expression of the transcriptional regulator Aire was unchanged, we observed downregulation of a set of PTA mRNAs. A set of miRNAs was also differentially expressed in these mice. The reconstruction of miRNA–mRNA interaction networks identified the controller miRNAs and predicted the PTA mRNA targets. Interestingly, the known Aire-dependent PTAs exhibited pronounced refractoriness in the networking interaction with miRNAs. This study reveals the existence of a new mechanism in mTECs, and this mechanism may have importance in the control of self-tolerance.</abstract><cop>Netherlands</cop><pub>Elsevier GmbH</pub><pmid>25220732</pmid><doi>10.1016/j.imbio.2014.08.015</doi><tpages>10</tpages></addata></record> |
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| subjects | Advanced Basic Science Aire gene AIRE Protein Allergy and Immunology Animals Animals, Newborn Antigens - genetics Antigens - immunology Cluster Analysis Epistasis, Genetic Epithelial Cells - immunology Epithelial Cells - metabolism Female Gene Expression Profiling Gene Expression Regulation Gene Regulatory Networks Mice microRNAs MicroRNAs - genetics Peripheral tissue antigen Post-transcriptional control RNA Interference RNA, Messenger - genetics Self Tolerance Thymus Thymus Gland - cytology Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic |
| title | Aire-dependent peripheral tissue antigen mRNAs in mTEC cells feature networking refractoriness to microRNA interaction |
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