Mixed culture biofilms of Salmonella Typhimurium and cultivable indigenous microorganisms on lettuce show enhanced resistance of their sessile cells to cold oxygen plasma
Control of foodborne pathogens in fresh produce is crucial for food safety, and numerous Salmonella Typhimurium (ST) outbreaks have been reported already. The present study was done to assess effectiveness of cold oxygen plasma (COP) against biofilms of ST mixed with cultivable indigenous microorgan...
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| Veröffentlicht in: | Food microbiology 2015-04, Vol.46, p.383-394 |
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| creator | Jahid, Iqbal Kabir Han, Noori Zhang, Cheng-Yi Ha, Sang-Do |
| description | Control of foodborne pathogens in fresh produce is crucial for food safety, and numerous Salmonella Typhimurium (ST) outbreaks have been reported already. The present study was done to assess effectiveness of cold oxygen plasma (COP) against biofilms of ST mixed with cultivable indigenous microorganisms (CIM). ST and CIM were grown at 15 °C as monocultures and mixed cultures for planktonic state, biofilm on stainless steel, and lettuce leaves. Thereafter, the samples were treated with COP and surviving populations were counted using plate counting methods. Biofilms and stomatal colonization were examined using field emission scanning electron microscopy (FESEM) and food quality was assessed after treatment. Mixed cultures of ST and CIM showed an antagonistic interaction on lettuce but not on SS or in planktonic state. Mixed cultures showed significantly (p |
| doi_str_mv | 10.1016/j.fm.2014.08.003 |
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•Salmonella Typhimurium and resident microflora on lettuce show competitive action.•Mixed biofilms are more resistant than monocultures to cold oxygen plasma (COP).•COP switches Salmonella Typhimurium from smooth to rugose.•COP is ineffective to reduce biofilms population from lettuce.</description><identifier>ISSN: 0740-0020</identifier><identifier>EISSN: 1095-9998</identifier><identifier>DOI: 10.1016/j.fm.2014.08.003</identifier><identifier>PMID: 25475308</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Biofilms - drug effects ; Cold oxygen plasma ; Cold Temperature ; Indigenous microorganisms ; Lactuca - microbiology ; Mixed culture biofilms ; Oxygen - chemistry ; Oxygen - pharmacology ; Plant Leaves - microbiology ; Plasma Gases - pharmacology ; Salmonella Typhimurium ; Salmonella typhimurium - drug effects ; Salmonella typhimurium - growth & development ; Salmonella typhimurium - physiology ; Weibull model</subject><ispartof>Food microbiology, 2015-04, Vol.46, p.383-394</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-38b895ed8f27a0c12d0e717234ecb24a7cb4b0306c6e25304b6886d8ee719eef3</citedby><cites>FETCH-LOGICAL-c383t-38b895ed8f27a0c12d0e717234ecb24a7cb4b0306c6e25304b6886d8ee719eef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0740002014002020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25475308$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jahid, Iqbal Kabir</creatorcontrib><creatorcontrib>Han, Noori</creatorcontrib><creatorcontrib>Zhang, Cheng-Yi</creatorcontrib><creatorcontrib>Ha, Sang-Do</creatorcontrib><title>Mixed culture biofilms of Salmonella Typhimurium and cultivable indigenous microorganisms on lettuce show enhanced resistance of their sessile cells to cold oxygen plasma</title><title>Food microbiology</title><addtitle>Food Microbiol</addtitle><description>Control of foodborne pathogens in fresh produce is crucial for food safety, and numerous Salmonella Typhimurium (ST) outbreaks have been reported already. The present study was done to assess effectiveness of cold oxygen plasma (COP) against biofilms of ST mixed with cultivable indigenous microorganisms (CIM). ST and CIM were grown at 15 °C as monocultures and mixed cultures for planktonic state, biofilm on stainless steel, and lettuce leaves. Thereafter, the samples were treated with COP and surviving populations were counted using plate counting methods. Biofilms and stomatal colonization were examined using field emission scanning electron microscopy (FESEM) and food quality was assessed after treatment. Mixed cultures of ST and CIM showed an antagonistic interaction on lettuce but not on SS or in planktonic state. Mixed cultures showed significantly (p < 0.05) greater resistance to COP compared to monoculture biofilms on lettuce but not on SS or planktonic state. Shift from smooth to rugose colony type was found for planktonic and for biofilms on SS but not on lettuce for ST. Mixed culture biofilms colonized stomata on the inside as demonstrated by FESEM. Although, lettuce quality was not affected by COP, this technology has to be optimized for further development of the successful inactivation of complex multispecies biofilm structures presented by real food environment.
•Salmonella Typhimurium and resident microflora on lettuce show competitive action.•Mixed biofilms are more resistant than monocultures to cold oxygen plasma (COP).•COP switches Salmonella Typhimurium from smooth to rugose.•COP is ineffective to reduce biofilms population from lettuce.</description><subject>Biofilms - drug effects</subject><subject>Cold oxygen plasma</subject><subject>Cold Temperature</subject><subject>Indigenous microorganisms</subject><subject>Lactuca - microbiology</subject><subject>Mixed culture biofilms</subject><subject>Oxygen - chemistry</subject><subject>Oxygen - pharmacology</subject><subject>Plant Leaves - microbiology</subject><subject>Plasma Gases - pharmacology</subject><subject>Salmonella Typhimurium</subject><subject>Salmonella typhimurium - drug effects</subject><subject>Salmonella typhimurium - growth & development</subject><subject>Salmonella typhimurium - physiology</subject><subject>Weibull model</subject><issn>0740-0020</issn><issn>1095-9998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkT2P1DAQhi0E4paDngq5pEkYx_lw6NCJL-kQBUdtOc7kdlZ2vNjJcfuX-JU42oMOicounnnGfl_GXgooBYj2zaGcfFmBqEtQJYB8xHYC-qbo-149ZjvoaigAKrhgz1I6AAjRyP4pu6iaumskqB379YXuceR2dcsakQ8UJnI-8TDxb8b5MKNzht-cjnvya6TVczOfcbozg0NO80i3OIc1cU82hhBvzUxpU8zc4bKsFnnah58c572ZbV4WMVFatvu2ZtkjRZ4wJco6m_clvgRugxt5uD9lNz86k7x5zp5MxiV88XBesu8f3t9cfSquv378fPXuurBSyaWQalB9g6Oaqs6AFdUI2ImukjXaoapNZ4d6AAmtbbHKIdRDq1Q7KsxUjzjJS_b67D3G8GPFtGhPaXuXmTF_U4u27nKobdf-ByrrSomuVRmFM5ozSinipI-RvIknLUBvZeqDnrzeytSgdC4zj7x6sK-Dx_HvwJ_2MvD2DGCO444w6mQJt4wpol30GOjf9t8SxrLu</recordid><startdate>201504</startdate><enddate>201504</enddate><creator>Jahid, Iqbal Kabir</creator><creator>Han, Noori</creator><creator>Zhang, Cheng-Yi</creator><creator>Ha, Sang-Do</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201504</creationdate><title>Mixed culture biofilms of Salmonella Typhimurium and cultivable indigenous microorganisms on lettuce show enhanced resistance of their sessile cells to cold oxygen plasma</title><author>Jahid, Iqbal Kabir ; Han, Noori ; Zhang, Cheng-Yi ; Ha, Sang-Do</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-38b895ed8f27a0c12d0e717234ecb24a7cb4b0306c6e25304b6886d8ee719eef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biofilms - drug effects</topic><topic>Cold oxygen plasma</topic><topic>Cold Temperature</topic><topic>Indigenous microorganisms</topic><topic>Lactuca - microbiology</topic><topic>Mixed culture biofilms</topic><topic>Oxygen - chemistry</topic><topic>Oxygen - pharmacology</topic><topic>Plant Leaves - microbiology</topic><topic>Plasma Gases - pharmacology</topic><topic>Salmonella Typhimurium</topic><topic>Salmonella typhimurium - drug effects</topic><topic>Salmonella typhimurium - growth & development</topic><topic>Salmonella typhimurium - physiology</topic><topic>Weibull model</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jahid, Iqbal Kabir</creatorcontrib><creatorcontrib>Han, Noori</creatorcontrib><creatorcontrib>Zhang, Cheng-Yi</creatorcontrib><creatorcontrib>Ha, Sang-Do</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jahid, Iqbal Kabir</au><au>Han, Noori</au><au>Zhang, Cheng-Yi</au><au>Ha, Sang-Do</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mixed culture biofilms of Salmonella Typhimurium and cultivable indigenous microorganisms on lettuce show enhanced resistance of their sessile cells to cold oxygen plasma</atitle><jtitle>Food microbiology</jtitle><addtitle>Food Microbiol</addtitle><date>2015-04</date><risdate>2015</risdate><volume>46</volume><spage>383</spage><epage>394</epage><pages>383-394</pages><issn>0740-0020</issn><eissn>1095-9998</eissn><abstract>Control of foodborne pathogens in fresh produce is crucial for food safety, and numerous Salmonella Typhimurium (ST) outbreaks have been reported already. The present study was done to assess effectiveness of cold oxygen plasma (COP) against biofilms of ST mixed with cultivable indigenous microorganisms (CIM). ST and CIM were grown at 15 °C as monocultures and mixed cultures for planktonic state, biofilm on stainless steel, and lettuce leaves. Thereafter, the samples were treated with COP and surviving populations were counted using plate counting methods. Biofilms and stomatal colonization were examined using field emission scanning electron microscopy (FESEM) and food quality was assessed after treatment. Mixed cultures of ST and CIM showed an antagonistic interaction on lettuce but not on SS or in planktonic state. Mixed cultures showed significantly (p < 0.05) greater resistance to COP compared to monoculture biofilms on lettuce but not on SS or planktonic state. Shift from smooth to rugose colony type was found for planktonic and for biofilms on SS but not on lettuce for ST. Mixed culture biofilms colonized stomata on the inside as demonstrated by FESEM. Although, lettuce quality was not affected by COP, this technology has to be optimized for further development of the successful inactivation of complex multispecies biofilm structures presented by real food environment.
•Salmonella Typhimurium and resident microflora on lettuce show competitive action.•Mixed biofilms are more resistant than monocultures to cold oxygen plasma (COP).•COP switches Salmonella Typhimurium from smooth to rugose.•COP is ineffective to reduce biofilms population from lettuce.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25475308</pmid><doi>10.1016/j.fm.2014.08.003</doi><tpages>12</tpages></addata></record> |
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| subjects | Biofilms - drug effects Cold oxygen plasma Cold Temperature Indigenous microorganisms Lactuca - microbiology Mixed culture biofilms Oxygen - chemistry Oxygen - pharmacology Plant Leaves - microbiology Plasma Gases - pharmacology Salmonella Typhimurium Salmonella typhimurium - drug effects Salmonella typhimurium - growth & development Salmonella typhimurium - physiology Weibull model |
| title | Mixed culture biofilms of Salmonella Typhimurium and cultivable indigenous microorganisms on lettuce show enhanced resistance of their sessile cells to cold oxygen plasma |
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