Sequence polymorphisms in Zmisa2 gene are significantly associated with starch pasting and gelatinization properties in maize (Zea mays L.)
Researches on the genetic basis of starch pasting and gelatinization properties will provide foundation of maize improvement for quality, feed and industrial applications. Maize gene Zmisa2 , encoding an isoamylase-type starch debranching enzyme, plays important roles in starch biosynthesis. In this...
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| Veröffentlicht in: | Molecular breeding 2014-12, Vol.34 (4), p.1833-1842 |
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| creator | Yang, Zefeng Zhang, Enying Jiang, Ying Xu, Shuhui Pan, Liang Chen, Qing Xu, Chenwu |
| description | Researches on the genetic basis of starch pasting and gelatinization properties will provide foundation of maize improvement for quality, feed and industrial applications. Maize gene
Zmisa2
, encoding an isoamylase-type starch debranching enzyme, plays important roles in starch biosynthesis. In this study, the genomic sequences of the gene
Zmisa2
in 72 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. In addition, seven pasting and four gelatinization properties of maize were measured for the tested inbred lines using rapid visco analyzer and differential scanning calorimeter, respectively. A total of 99 sequence variants, including 91 SNPs and 8 indels, were identified at the promoter and coding regions of this gene. Although the frequency of polymorphism in promoter region is much higher than that of coding region, the SNPs in the coding region of maize gene
Zmisa2
classified this gene into 21 haplotypes, which encode 11 different ISA2 proteins. Furthermore, the association of the variants of
Zmisa2
gene with maize starch pasting and gelatinization properties was estimated, and the results revealed that seven SNPs in coding region, including four nonsynonymous sites, were significantly associated with phenotypic variations of pasting time and enthalpy of transition (Δ
H
). These results suggested that the polymorphism in maize
Zmisa2
locus could be used in molecular marker-assisted selection for improvement of quality in maize breeding programs. |
| doi_str_mv | 10.1007/s11032-014-0142-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1647002370</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2259416559</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-c30c37ce02c7c1f86b18d67adf8d94dc5088d151d9ee21f621f4af6115e9f7633</originalsourceid><addsrcrecordid>eNp1UU2LFDEQbUTBdfUHeAt4WQ-9ppLuJH2UxdWFAQ_qZS8hpqtnsnSn21QPMvMX_NPWOMKC4OHVB7z3qOJV1WuQ1yClfUcAUqtaQnOCqo9Pqgtorao769xTnrWTtbaNfl69IHqQrOmMuah-fcEfe8wRxTKPh2kuyy7RRCJlcT8lCkpsMaMIBQWlbU5DiiGv40EEojmmsGIvfqZ1J2gNJe7EEmhNeStC7lk5Bl7SkeucxVLmBcua8I_7FNIRxdU9Bh4PJDbXb19Wz4YwEr762y-rb7cfvt58qjefP97dvN_UUTu1cpVR24hSRRthcOY7uN7Y0A-u75o-ttK5HlroO0QFg2E0YTAALXaDNVpfVldnX76In6fV86cRxzFknPfkwTRWSqWtZOqbf6gP875kvs4r1XYNmLbtmAVnViwzUcHBLyVNoRw8SH-Kx5_j8RzNCcofWaPOGmJu3mJ5dP6_6DfEwpTx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2259416559</pqid></control><display><type>article</type><title>Sequence polymorphisms in Zmisa2 gene are significantly associated with starch pasting and gelatinization properties in maize (Zea mays L.)</title><source>Springer Nature - Complete Springer Journals</source><creator>Yang, Zefeng ; Zhang, Enying ; Jiang, Ying ; Xu, Shuhui ; Pan, Liang ; Chen, Qing ; Xu, Chenwu</creator><creatorcontrib>Yang, Zefeng ; Zhang, Enying ; Jiang, Ying ; Xu, Shuhui ; Pan, Liang ; Chen, Qing ; Xu, Chenwu</creatorcontrib><description>Researches on the genetic basis of starch pasting and gelatinization properties will provide foundation of maize improvement for quality, feed and industrial applications. Maize gene
Zmisa2
, encoding an isoamylase-type starch debranching enzyme, plays important roles in starch biosynthesis. In this study, the genomic sequences of the gene
Zmisa2
in 72 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. In addition, seven pasting and four gelatinization properties of maize were measured for the tested inbred lines using rapid visco analyzer and differential scanning calorimeter, respectively. A total of 99 sequence variants, including 91 SNPs and 8 indels, were identified at the promoter and coding regions of this gene. Although the frequency of polymorphism in promoter region is much higher than that of coding region, the SNPs in the coding region of maize gene
Zmisa2
classified this gene into 21 haplotypes, which encode 11 different ISA2 proteins. Furthermore, the association of the variants of
Zmisa2
gene with maize starch pasting and gelatinization properties was estimated, and the results revealed that seven SNPs in coding region, including four nonsynonymous sites, were significantly associated with phenotypic variations of pasting time and enthalpy of transition (Δ
H
). These results suggested that the polymorphism in maize
Zmisa2
locus could be used in molecular marker-assisted selection for improvement of quality in maize breeding programs.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-014-0142-z</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Corn ; Enthalpy ; Gene polymorphism ; Gene sequencing ; Haplotypes ; Inbreeding ; Industrial applications ; Isoamylase ; Life Sciences ; Marker-assisted selection ; Molecular biology ; Nucleotides ; Phenotypic variations ; Plant biology ; Plant breeding ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polymorphism ; Properties (attributes) ; Single-nucleotide polymorphism ; Starch ; Zea mays</subject><ispartof>Molecular breeding, 2014-12, Vol.34 (4), p.1833-1842</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><rights>Molecular Breeding is a copyright of Springer, (2014). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-c30c37ce02c7c1f86b18d67adf8d94dc5088d151d9ee21f621f4af6115e9f7633</citedby><cites>FETCH-LOGICAL-c382t-c30c37ce02c7c1f86b18d67adf8d94dc5088d151d9ee21f621f4af6115e9f7633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-014-0142-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-014-0142-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Yang, Zefeng</creatorcontrib><creatorcontrib>Zhang, Enying</creatorcontrib><creatorcontrib>Jiang, Ying</creatorcontrib><creatorcontrib>Xu, Shuhui</creatorcontrib><creatorcontrib>Pan, Liang</creatorcontrib><creatorcontrib>Chen, Qing</creatorcontrib><creatorcontrib>Xu, Chenwu</creatorcontrib><title>Sequence polymorphisms in Zmisa2 gene are significantly associated with starch pasting and gelatinization properties in maize (Zea mays L.)</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>Researches on the genetic basis of starch pasting and gelatinization properties will provide foundation of maize improvement for quality, feed and industrial applications. Maize gene
Zmisa2
, encoding an isoamylase-type starch debranching enzyme, plays important roles in starch biosynthesis. In this study, the genomic sequences of the gene
Zmisa2
in 72 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. In addition, seven pasting and four gelatinization properties of maize were measured for the tested inbred lines using rapid visco analyzer and differential scanning calorimeter, respectively. A total of 99 sequence variants, including 91 SNPs and 8 indels, were identified at the promoter and coding regions of this gene. Although the frequency of polymorphism in promoter region is much higher than that of coding region, the SNPs in the coding region of maize gene
Zmisa2
classified this gene into 21 haplotypes, which encode 11 different ISA2 proteins. Furthermore, the association of the variants of
Zmisa2
gene with maize starch pasting and gelatinization properties was estimated, and the results revealed that seven SNPs in coding region, including four nonsynonymous sites, were significantly associated with phenotypic variations of pasting time and enthalpy of transition (Δ
H
). These results suggested that the polymorphism in maize
Zmisa2
locus could be used in molecular marker-assisted selection for improvement of quality in maize breeding programs.</description><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Corn</subject><subject>Enthalpy</subject><subject>Gene polymorphism</subject><subject>Gene sequencing</subject><subject>Haplotypes</subject><subject>Inbreeding</subject><subject>Industrial applications</subject><subject>Isoamylase</subject><subject>Life Sciences</subject><subject>Marker-assisted selection</subject><subject>Molecular biology</subject><subject>Nucleotides</subject><subject>Phenotypic variations</subject><subject>Plant biology</subject><subject>Plant breeding</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polymorphism</subject><subject>Properties (attributes)</subject><subject>Single-nucleotide polymorphism</subject><subject>Starch</subject><subject>Zea mays</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UU2LFDEQbUTBdfUHeAt4WQ-9ppLuJH2UxdWFAQ_qZS8hpqtnsnSn21QPMvMX_NPWOMKC4OHVB7z3qOJV1WuQ1yClfUcAUqtaQnOCqo9Pqgtorao769xTnrWTtbaNfl69IHqQrOmMuah-fcEfe8wRxTKPh2kuyy7RRCJlcT8lCkpsMaMIBQWlbU5DiiGv40EEojmmsGIvfqZ1J2gNJe7EEmhNeStC7lk5Bl7SkeucxVLmBcua8I_7FNIRxdU9Bh4PJDbXb19Wz4YwEr762y-rb7cfvt58qjefP97dvN_UUTu1cpVR24hSRRthcOY7uN7Y0A-u75o-ttK5HlroO0QFg2E0YTAALXaDNVpfVldnX76In6fV86cRxzFknPfkwTRWSqWtZOqbf6gP875kvs4r1XYNmLbtmAVnViwzUcHBLyVNoRw8SH-Kx5_j8RzNCcofWaPOGmJu3mJ5dP6_6DfEwpTx</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Yang, Zefeng</creator><creator>Zhang, Enying</creator><creator>Jiang, Ying</creator><creator>Xu, Shuhui</creator><creator>Pan, Liang</creator><creator>Chen, Qing</creator><creator>Xu, Chenwu</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20141201</creationdate><title>Sequence polymorphisms in Zmisa2 gene are significantly associated with starch pasting and gelatinization properties in maize (Zea mays L.)</title><author>Yang, Zefeng ; Zhang, Enying ; Jiang, Ying ; Xu, Shuhui ; Pan, Liang ; Chen, Qing ; Xu, Chenwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-c30c37ce02c7c1f86b18d67adf8d94dc5088d151d9ee21f621f4af6115e9f7633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Corn</topic><topic>Enthalpy</topic><topic>Gene polymorphism</topic><topic>Gene sequencing</topic><topic>Haplotypes</topic><topic>Inbreeding</topic><topic>Industrial applications</topic><topic>Isoamylase</topic><topic>Life Sciences</topic><topic>Marker-assisted selection</topic><topic>Molecular biology</topic><topic>Nucleotides</topic><topic>Phenotypic variations</topic><topic>Plant biology</topic><topic>Plant breeding</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polymorphism</topic><topic>Properties (attributes)</topic><topic>Single-nucleotide polymorphism</topic><topic>Starch</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Zefeng</creatorcontrib><creatorcontrib>Zhang, Enying</creatorcontrib><creatorcontrib>Jiang, Ying</creatorcontrib><creatorcontrib>Xu, Shuhui</creatorcontrib><creatorcontrib>Pan, Liang</creatorcontrib><creatorcontrib>Chen, Qing</creatorcontrib><creatorcontrib>Xu, Chenwu</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Zefeng</au><au>Zhang, Enying</au><au>Jiang, Ying</au><au>Xu, Shuhui</au><au>Pan, Liang</au><au>Chen, Qing</au><au>Xu, Chenwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence polymorphisms in Zmisa2 gene are significantly associated with starch pasting and gelatinization properties in maize (Zea mays L.)</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2014-12-01</date><risdate>2014</risdate><volume>34</volume><issue>4</issue><spage>1833</spage><epage>1842</epage><pages>1833-1842</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Researches on the genetic basis of starch pasting and gelatinization properties will provide foundation of maize improvement for quality, feed and industrial applications. Maize gene
Zmisa2
, encoding an isoamylase-type starch debranching enzyme, plays important roles in starch biosynthesis. In this study, the genomic sequences of the gene
Zmisa2
in 72 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. In addition, seven pasting and four gelatinization properties of maize were measured for the tested inbred lines using rapid visco analyzer and differential scanning calorimeter, respectively. A total of 99 sequence variants, including 91 SNPs and 8 indels, were identified at the promoter and coding regions of this gene. Although the frequency of polymorphism in promoter region is much higher than that of coding region, the SNPs in the coding region of maize gene
Zmisa2
classified this gene into 21 haplotypes, which encode 11 different ISA2 proteins. Furthermore, the association of the variants of
Zmisa2
gene with maize starch pasting and gelatinization properties was estimated, and the results revealed that seven SNPs in coding region, including four nonsynonymous sites, were significantly associated with phenotypic variations of pasting time and enthalpy of transition (Δ
H
). These results suggested that the polymorphism in maize
Zmisa2
locus could be used in molecular marker-assisted selection for improvement of quality in maize breeding programs.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11032-014-0142-z</doi><tpages>10</tpages></addata></record> |
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| ispartof | Molecular breeding, 2014-12, Vol.34 (4), p.1833-1842 |
| issn | 1380-3743 1572-9788 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biomedical and Life Sciences Biosynthesis Biotechnology Corn Enthalpy Gene polymorphism Gene sequencing Haplotypes Inbreeding Industrial applications Isoamylase Life Sciences Marker-assisted selection Molecular biology Nucleotides Phenotypic variations Plant biology Plant breeding Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polymorphism Properties (attributes) Single-nucleotide polymorphism Starch Zea mays |
| title | Sequence polymorphisms in Zmisa2 gene are significantly associated with starch pasting and gelatinization properties in maize (Zea mays L.) |
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