Action of diphtheria toxin does not depend on the induction of large, stable pores across biological membranes
Vero cells exposed to diphtheria toxin at pH 4.5 leak monovalent cations but not amino acids or phosphorylated metabolites; affected cells do not take up trypan blue. Monovalent cation leakage is inhibited by 1 mM Cd2+, but not by 1 mM Zn2+ or Ca2+. Cd2+ blocks calcein leakage from liposomes and clo...
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| Veröffentlicht in: | The Journal of membrane biology 1990-01, Vol.113 (1), p.67-74 |
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| container_title | The Journal of membrane biology |
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| creator | Alder, G M Bashford, C L Pasternak, C A |
| description | Vero cells exposed to diphtheria toxin at pH 4.5 leak monovalent cations but not amino acids or phosphorylated metabolites; affected cells do not take up trypan blue. Monovalent cation leakage is inhibited by 1 mM Cd2+, but not by 1 mM Zn2+ or Ca2+. Cd2+ blocks calcein leakage from liposomes and closes diphtheria toxin-induced channels in lipid bilayers. It is concluded that translocation of the A fragment of diphtheria toxin across biological membranes does not depend on the formation of large stable pores, but that small Cd2(+)-sensitive pores may play a role. |
| doi_str_mv | 10.1007/BF01869607 |
| format | Article |
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Monovalent cation leakage is inhibited by 1 mM Cd2+, but not by 1 mM Zn2+ or Ca2+. Cd2+ blocks calcein leakage from liposomes and closes diphtheria toxin-induced channels in lipid bilayers. It is concluded that translocation of the A fragment of diphtheria toxin across biological membranes does not depend on the formation of large stable pores, but that small Cd2(+)-sensitive pores may play a role.</description><identifier>ISSN: 0022-2631</identifier><identifier>EISSN: 1432-1424</identifier><identifier>DOI: 10.1007/BF01869607</identifier><identifier>PMID: 2304072</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Cadmium - pharmacology ; Cell Membrane - metabolism ; cell membranes ; Corynebacterium diphtheriae ; Cytotoxins - pharmacology ; cytoxicity ; Diphtheria Toxin - pharmacology ; Fluoresceins - metabolism ; induction ; Kinetics ; Lipid Bilayers - metabolism ; Liposomes - metabolism ; Permeability ; pores ; Potassium - metabolism ; Protein Biosynthesis ; requirements ; Sodium - metabolism ; toxins ; Vero Cells</subject><ispartof>The Journal of membrane biology, 1990-01, Vol.113 (1), p.67-74</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-30cdc80f5bc8fc26aea98bb3fdfb00990e38aa76a111c90276e2bdf23fa913e43</citedby><cites>FETCH-LOGICAL-c313t-30cdc80f5bc8fc26aea98bb3fdfb00990e38aa76a111c90276e2bdf23fa913e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2304072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alder, G M</creatorcontrib><creatorcontrib>Bashford, C L</creatorcontrib><creatorcontrib>Pasternak, C A</creatorcontrib><title>Action of diphtheria toxin does not depend on the induction of large, stable pores across biological membranes</title><title>The Journal of membrane biology</title><addtitle>J Membr Biol</addtitle><description>Vero cells exposed to diphtheria toxin at pH 4.5 leak monovalent cations but not amino acids or phosphorylated metabolites; affected cells do not take up trypan blue. 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| identifier | ISSN: 0022-2631 |
| ispartof | The Journal of membrane biology, 1990-01, Vol.113 (1), p.67-74 |
| issn | 0022-2631 1432-1424 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Cadmium - pharmacology Cell Membrane - metabolism cell membranes Corynebacterium diphtheriae Cytotoxins - pharmacology cytoxicity Diphtheria Toxin - pharmacology Fluoresceins - metabolism induction Kinetics Lipid Bilayers - metabolism Liposomes - metabolism Permeability pores Potassium - metabolism Protein Biosynthesis requirements Sodium - metabolism toxins Vero Cells |
| title | Action of diphtheria toxin does not depend on the induction of large, stable pores across biological membranes |
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