The expression of an aquaporin promoter from Mesembryanthemum crystallinum in tobacco [Nicotiana tabacum]
The promoter region of the MipB gene encoding an aquaporin from Mesembryanthemum crystallinum was isolated and used in a transcriptional fusion to control uidA expression in tobacco. The sequence of the promoter was determined for 2 kb upstream of the translation initiation site. Three start sites w...
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| Veröffentlicht in: | Plant and cell physiology 1997-12, Vol.38 (12), p.1326-1332 |
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| container_title | Plant and cell physiology |
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| creator | Yamada, S. (University of Arizona, Tucson (USA)) Nelson, D.E Ley, E Marquez, S Bohnert, H |
| description | The promoter region of the MipB gene encoding an aquaporin from Mesembryanthemum crystallinum was isolated and used in a transcriptional fusion to control uidA expression in tobacco. The sequence of the promoter was determined for 2 kb upstream of the translation initiation site. Three start sites were utilized with approximately equal frequency, located 176, 170 and 161 bases, respectively, upstream of the translation initiation site. As judged by analysis of GUS expression, promoter MIpB retains its specificity in transgenic tobacco. In germinating seedlings, all cells showed GUS expression of different intensities with the strongest signals in root meristems. In older seedlings, GUS staining was observed in rapidly expanding cells -- root and apical meristem, and lateral root primordia. In mature plants, strong GUS activity was located to glandular trichomes, subepidermal cells of the stem and petioles, to cells surrounding vascular tissues as well as in xylem parenchyma cells. In immature floral organs, GUS expression was strong in sepals, petals, stamen, and pistil. The intensity declined as they matured. In general, this promoter was active in rapidly expanding cells and cells with high water flux capacity, especially in the xylem parenchyma |
| doi_str_mv | 10.1093/oxfordjournals.pcp.a029125 |
| format | Article |
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(University of Arizona, Tucson (USA)) ; Nelson, D.E ; Ley, E ; Marquez, S ; Bohnert, H</creator><creatorcontrib>Yamada, S. (University of Arizona, Tucson (USA)) ; Nelson, D.E ; Ley, E ; Marquez, S ; Bohnert, H</creatorcontrib><description>The promoter region of the MipB gene encoding an aquaporin from Mesembryanthemum crystallinum was isolated and used in a transcriptional fusion to control uidA expression in tobacco. The sequence of the promoter was determined for 2 kb upstream of the translation initiation site. Three start sites were utilized with approximately equal frequency, located 176, 170 and 161 bases, respectively, upstream of the translation initiation site. As judged by analysis of GUS expression, promoter MIpB retains its specificity in transgenic tobacco. In germinating seedlings, all cells showed GUS expression of different intensities with the strongest signals in root meristems. In older seedlings, GUS staining was observed in rapidly expanding cells -- root and apical meristem, and lateral root primordia. In mature plants, strong GUS activity was located to glandular trichomes, subepidermal cells of the stem and petioles, to cells surrounding vascular tissues as well as in xylem parenchyma cells. In immature floral organs, GUS expression was strong in sepals, petals, stamen, and pistil. The intensity declined as they matured. In general, this promoter was active in rapidly expanding cells and cells with high water flux capacity, especially in the xylem parenchyma</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/oxfordjournals.pcp.a029125</identifier><identifier>PMID: 9522465</identifier><identifier>CODEN: PCPHA5</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Aquaporin ; Aquaporins ; Biological and medical sciences ; Cell physiology ; Cloning, Molecular ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE EXPRESSION ; Glucuronidase - genetics ; Ion Channels - genetics ; MESEMBRYANTHEMUM ; Mesembryanthemum crystallinum ; Nicotiana ; NICOTIANA TABACUM ; Plant physiology and development ; Plant Proteins - genetics ; Plants - genetics ; Plants, Genetically Modified ; Plants, Toxic ; Plasma membrane and permeation ; Promoter analysis ; Promoter Regions, Genetic ; PROTEINAS ; PROTEINE ; PROTEINS ; TRANSCRIPCION ; TRANSCRIPTION ; Transcription, Genetic ; uidA expression</subject><ispartof>Plant and cell physiology, 1997-12, Vol.38 (12), p.1326-1332</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-90beabde222d1637010e65ddf3cc5b734b4807452a0d4f407c4f8dc7a8ac9ea23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2222466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9522465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamada, S. (University of Arizona, Tucson (USA))</creatorcontrib><creatorcontrib>Nelson, D.E</creatorcontrib><creatorcontrib>Ley, E</creatorcontrib><creatorcontrib>Marquez, S</creatorcontrib><creatorcontrib>Bohnert, H</creatorcontrib><title>The expression of an aquaporin promoter from Mesembryanthemum crystallinum in tobacco [Nicotiana tabacum]</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>The promoter region of the MipB gene encoding an aquaporin from Mesembryanthemum crystallinum was isolated and used in a transcriptional fusion to control uidA expression in tobacco. The sequence of the promoter was determined for 2 kb upstream of the translation initiation site. Three start sites were utilized with approximately equal frequency, located 176, 170 and 161 bases, respectively, upstream of the translation initiation site. As judged by analysis of GUS expression, promoter MIpB retains its specificity in transgenic tobacco. In germinating seedlings, all cells showed GUS expression of different intensities with the strongest signals in root meristems. In older seedlings, GUS staining was observed in rapidly expanding cells -- root and apical meristem, and lateral root primordia. In mature plants, strong GUS activity was located to glandular trichomes, subepidermal cells of the stem and petioles, to cells surrounding vascular tissues as well as in xylem parenchyma cells. In immature floral organs, GUS expression was strong in sepals, petals, stamen, and pistil. The intensity declined as they matured. In general, this promoter was active in rapidly expanding cells and cells with high water flux capacity, especially in the xylem parenchyma</description><subject>Aquaporin</subject><subject>Aquaporins</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Cloning, Molecular</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE EXPRESSION</subject><subject>Glucuronidase - genetics</subject><subject>Ion Channels - genetics</subject><subject>MESEMBRYANTHEMUM</subject><subject>Mesembryanthemum crystallinum</subject><subject>Nicotiana</subject><subject>NICOTIANA TABACUM</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plants - genetics</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>Plasma membrane and permeation</subject><subject>Promoter analysis</subject><subject>Promoter Regions, Genetic</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><subject>Transcription, Genetic</subject><subject>uidA expression</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkdtu1DAQhi0EKkvhEZAshLjL1sc44Q6tSg8qbS-KhIqQNXFs6pLEqZ1Iu2-Pq40WceXxzDczv38j9IGSNSU1PwlbF2L7GOY4QJfWoxnXQFhNmXyBVlQoWtRE8pdoRQhnBVEVfY3epPRISI45OUJHtWRMlHKF_N2DxXY7RpuSDwMODsOA4WmGMUQ_4DGGPkw2YpcD_M0m2zdxB8P0YPu5xybu0gRd54d8yfgUGjAm4J_X3oTJwwB4gpya-19v0SuX1dp3y3mMvn89vducF1c3ZxebL1eFkZROWXljoWktY6ylJVeEElvKtnXcGNkoLhpRESUkA9IKJ4gywlWtUVCBqS0wfow-7edm6U-zTZPufTK262CwYU6alkKWTIkMft6DJoaUonV6jL6HuNOU6Gef9f8-6-yzXnzOze-XLXPT2_bQuhib6x-XOiQDnYswGJ8OWH5dxsqMFXvMp8luD2WIf3SpuJL6_Me9vj8TG1lmUbf_1joIGn7HPPLyltZ1lX-aU8r_AtoVp0E</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Yamada, S. (University of Arizona, Tucson (USA))</creator><creator>Nelson, D.E</creator><creator>Ley, E</creator><creator>Marquez, S</creator><creator>Bohnert, H</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19971201</creationdate><title>The expression of an aquaporin promoter from Mesembryanthemum crystallinum in tobacco [Nicotiana tabacum]</title><author>Yamada, S. (University of Arizona, Tucson (USA)) ; Nelson, D.E ; Ley, E ; Marquez, S ; Bohnert, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-90beabde222d1637010e65ddf3cc5b734b4807452a0d4f407c4f8dc7a8ac9ea23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Aquaporin</topic><topic>Aquaporins</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>Cloning, Molecular</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE EXPRESSION</topic><topic>Glucuronidase - genetics</topic><topic>Ion Channels - genetics</topic><topic>MESEMBRYANTHEMUM</topic><topic>Mesembryanthemum crystallinum</topic><topic>Nicotiana</topic><topic>NICOTIANA TABACUM</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plants - genetics</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Toxic</topic><topic>Plasma membrane and permeation</topic><topic>Promoter analysis</topic><topic>Promoter Regions, Genetic</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription, Genetic</topic><topic>uidA expression</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamada, S. (University of Arizona, Tucson (USA))</creatorcontrib><creatorcontrib>Nelson, D.E</creatorcontrib><creatorcontrib>Ley, E</creatorcontrib><creatorcontrib>Marquez, S</creatorcontrib><creatorcontrib>Bohnert, H</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamada, S. (University of Arizona, Tucson (USA))</au><au>Nelson, D.E</au><au>Ley, E</au><au>Marquez, S</au><au>Bohnert, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The expression of an aquaporin promoter from Mesembryanthemum crystallinum in tobacco [Nicotiana tabacum]</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>38</volume><issue>12</issue><spage>1326</spage><epage>1332</epage><pages>1326-1332</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><coden>PCPHA5</coden><abstract>The promoter region of the MipB gene encoding an aquaporin from Mesembryanthemum crystallinum was isolated and used in a transcriptional fusion to control uidA expression in tobacco. The sequence of the promoter was determined for 2 kb upstream of the translation initiation site. Three start sites were utilized with approximately equal frequency, located 176, 170 and 161 bases, respectively, upstream of the translation initiation site. As judged by analysis of GUS expression, promoter MIpB retains its specificity in transgenic tobacco. In germinating seedlings, all cells showed GUS expression of different intensities with the strongest signals in root meristems. In older seedlings, GUS staining was observed in rapidly expanding cells -- root and apical meristem, and lateral root primordia. In mature plants, strong GUS activity was located to glandular trichomes, subepidermal cells of the stem and petioles, to cells surrounding vascular tissues as well as in xylem parenchyma cells. In immature floral organs, GUS expression was strong in sepals, petals, stamen, and pistil. The intensity declined as they matured. In general, this promoter was active in rapidly expanding cells and cells with high water flux capacity, especially in the xylem parenchyma</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>9522465</pmid><doi>10.1093/oxfordjournals.pcp.a029125</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant and cell physiology, 1997-12, Vol.38 (12), p.1326-1332 |
| issn | 0032-0781 1471-9053 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan |
| subjects | Aquaporin Aquaporins Biological and medical sciences Cell physiology Cloning, Molecular EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION Glucuronidase - genetics Ion Channels - genetics MESEMBRYANTHEMUM Mesembryanthemum crystallinum Nicotiana NICOTIANA TABACUM Plant physiology and development Plant Proteins - genetics Plants - genetics Plants, Genetically Modified Plants, Toxic Plasma membrane and permeation Promoter analysis Promoter Regions, Genetic PROTEINAS PROTEINE PROTEINS TRANSCRIPCION TRANSCRIPTION Transcription, Genetic uidA expression |
| title | The expression of an aquaporin promoter from Mesembryanthemum crystallinum in tobacco [Nicotiana tabacum] |
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