Direct visualisation and quantification of cellular cytotoxicity using two colour fluorescence

A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated kil...

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Veröffentlicht in:	Journal of immunological methods 1992-01, Vol.156 (1), p.47-54
Hauptverfasser:	Kroesen, Bart-Jan, Mesander, G., ter Haar, J.G., The, T.H., de Leij, L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Activated T cell Biological and medical sciences Cellular cytotoxicity Fundamental and applied biological sciences. Psychology Fundamental immunology Lymphokine activated killer cell Membrane fluorescence Molecular immunology Natural killer cell Techniques
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Beschreibung
Zusammenfassung:	A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well as targeted T cell mediated cellular cytotoxicity were quantified using the fluorescence method and compared to results obtained with the 51chromium ( 51Cr) release assay. A good correlation was found after an assay period of 4–8 h indicating that the fluorescence method is a reliable alternative to the 51Cr release assay.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(92)90009-I