Purification and characterization of the SRS2 DNA helicase of the yeast Saccharomyces cerevisiae

The SRS2 gene of Saccharomyces cerevisiae was identified through mutational analysis as a suppressor of radiation-sensitive mutations in the error-prone repair pathway and by a hyper-recombination phenotype. Comparison of the derived amino acid sequence revealed the gene to have high homology to the...

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Veröffentlicht in:	The Journal of biological chemistry 1993-01, Vol.268 (2), p.1252-1259
Hauptverfasser:	LEI RONG, KLEIN, H. L
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - antagonists & inhibitors Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Cloning, Molecular DNA Helicases - genetics DNA Helicases - isolation & purification DNA Helicases - metabolism Enzymes and enzyme inhibitors Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Fungal Kinetics Miscellaneous Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides Plasmids Polymerase Chain Reaction - methods Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Restriction Mapping Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Substrate Specificity
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