Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on blood and spleen natural killer (NK) cell activity in the mouse

The immunotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were studied in male A/J mice after a loading dose of 5 micrograms TCDD/kg body wt. followed by 3 weekly maintenance doses of 1.42 micrograms TCDD/kg b.w. administered intraperitoneally. Tissue samples and immune cells were prepar...

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Veröffentlicht in:Toxicology letters 1992-05, Vol.60 (3), p.247-256
Hauptverfasser: FUNSETH, E, NILS-GUNNAR ILBÄCK
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description The immunotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were studied in male A/J mice after a loading dose of 5 micrograms TCDD/kg body wt. followed by 3 weekly maintenance doses of 1.42 micrograms TCDD/kg b.w. administered intraperitoneally. Tissue samples and immune cells were prepared on two occasions, i.e. on days 28 and 120 after the first injection of TCDD. This dose of TCDD evoked classical histological signs of liver damage and lipid accumulation, as well as thymic atrophy. Red (RBC) blood cell counts were significantly lowered in the TCDD group on day 28, but were normal on day 120. White (WBC) blood cell counts were normal in the TCDD group. Natural killer (NK) cell activity increased 3.4-fold (P less than 0.01) and 2.2-fold (P less than 0.01) in the blood and spleen, respectively, after 28 days, and these effects persisted on day 120. The increased NK-cell activity occurred concomitantly with a decreased proliferative response of spleen lymphocytes to the T-cell mitogen concanavalin A after both 28 (65%) and 120 days (58%). The proliferative response of spleen cells to the B-cell mitogen lipopolysaccharide seemed, however, unaffected. We have thus shown for the first time that TCDD induces an increased activity of NK cells that occurs simultaneously in the blood and spleen. This effect may indicate a general compensatory activation of the body's defences brought about by disturbances in the function of other arms of the immune system.
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Tissue samples and immune cells were prepared on two occasions, i.e. on days 28 and 120 after the first injection of TCDD. This dose of TCDD evoked classical histological signs of liver damage and lipid accumulation, as well as thymic atrophy. Red (RBC) blood cell counts were significantly lowered in the TCDD group on day 28, but were normal on day 120. White (WBC) blood cell counts were normal in the TCDD group. Natural killer (NK) cell activity increased 3.4-fold (P less than 0.01) and 2.2-fold (P less than 0.01) in the blood and spleen, respectively, after 28 days, and these effects persisted on day 120. The increased NK-cell activity occurred concomitantly with a decreased proliferative response of spleen lymphocytes to the T-cell mitogen concanavalin A after both 28 (65%) and 120 days (58%). The proliferative response of spleen cells to the B-cell mitogen lipopolysaccharide seemed, however, unaffected. 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Toxic occupational diseases</topic><topic>Killer Cells, Natural - drug effects</topic><topic>Killer Cells, Natural - physiology</topic><topic>Leukocyte Count - drug effects</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphoid Tissue - cytology</topic><topic>Lymphoid Tissue - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Polychlorinated Dibenzodioxins - blood</topic><topic>Polychlorinated Dibenzodioxins - toxicity</topic><topic>Spleen - cytology</topic><topic>Spleen - drug effects</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - physiology</topic><topic>Toxicology</topic><topic>Various organic compounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FUNSETH, E</creatorcontrib><creatorcontrib>NILS-GUNNAR ILBÄCK</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FUNSETH, E</au><au>NILS-GUNNAR ILBÄCK</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on blood and spleen natural killer (NK) cell activity in the mouse</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>1992-05</date><risdate>1992</risdate><volume>60</volume><issue>3</issue><spage>247</spage><epage>256</epage><pages>247-256</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>The immunotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were studied in male A/J mice after a loading dose of 5 micrograms TCDD/kg body wt. followed by 3 weekly maintenance doses of 1.42 micrograms TCDD/kg b.w. administered intraperitoneally. Tissue samples and immune cells were prepared on two occasions, i.e. on days 28 and 120 after the first injection of TCDD. This dose of TCDD evoked classical histological signs of liver damage and lipid accumulation, as well as thymic atrophy. Red (RBC) blood cell counts were significantly lowered in the TCDD group on day 28, but were normal on day 120. White (WBC) blood cell counts were normal in the TCDD group. Natural killer (NK) cell activity increased 3.4-fold (P less than 0.01) and 2.2-fold (P less than 0.01) in the blood and spleen, respectively, after 28 days, and these effects persisted on day 120. The increased NK-cell activity occurred concomitantly with a decreased proliferative response of spleen lymphocytes to the T-cell mitogen concanavalin A after both 28 (65%) and 120 days (58%). The proliferative response of spleen cells to the B-cell mitogen lipopolysaccharide seemed, however, unaffected. We have thus shown for the first time that TCDD induces an increased activity of NK cells that occurs simultaneously in the blood and spleen. This effect may indicate a general compensatory activation of the body's defences brought about by disturbances in the function of other arms of the immune system.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Science</pub><pmid>1595084</pmid><tpages>10</tpages></addata></record>
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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Animals
Biological and medical sciences
Body Weight - drug effects
Chemical and industrial products toxicology. Toxic occupational diseases
Killer Cells, Natural - drug effects
Killer Cells, Natural - physiology
Leukocyte Count - drug effects
Lymphocyte Activation - drug effects
Lymphoid Tissue - cytology
Lymphoid Tissue - drug effects
Male
Medical sciences
Mice
Mice, Inbred Strains
Polychlorinated Dibenzodioxins - blood
Polychlorinated Dibenzodioxins - toxicity
Spleen - cytology
Spleen - drug effects
T-Lymphocytes - drug effects
T-Lymphocytes - physiology
Toxicology
Various organic compounds
title Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on blood and spleen natural killer (NK) cell activity in the mouse
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