Full-Thickness Skin with Mature Hair Follicles Generated from Tissue Culture Expanded Human Cells
The goal of regenerative medicine is to reconstruct fully functional organs from tissue culture expanded human cells. In this study, we report a method for human reconstructed skin (hRSK) when starting with human cells. We implanted tissue culture expanded human epidermal and dermal cells into an ex...
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| Veröffentlicht in: | Tissue engineering. Part A 2014-12, Vol.20 (23-24), p.3314-3321 |
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| container_issue | 23-24 |
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| container_title | Tissue engineering. Part A |
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| creator | Wu, Xunwei Scott, Larry Washenik, Ken Stenn, Kurt |
| description | The goal of regenerative medicine is to reconstruct fully functional organs from tissue culture expanded human cells. In this study, we report a method for human reconstructed skin (hRSK) when starting with human cells. We implanted tissue culture expanded human epidermal and dermal cells into an excision wound on the back of immunodeficient mice. Pigmented skin covered the wound 4 weeks after implantation. Hair shafts were visible at 12 weeks and prominent at 14 weeks. Histologically, the hRSK comprises an intact epidermis and dermis with mature hair follicles, sebaceous glands and most notably, and unique to this system, subcutis. Morphogenesis, differentiation, and maturation of the hRSK mirror the human fetal process. Human antigen markers demonstrate that the constituent cells are of human origin for at least 6 months. The degree of new skin formation is most complete when using tissue culture expanded cells from fetal skin, but it also occurs with expanded newborn and adult cells; however, no appendages formed when we grafted both adult dermal and epidermal cells. The hRSK system promises to be valuable as a laboratory model for studying biological, pathological, and pharmaceutical problems of human skin. |
| doi_str_mv | 10.1089/ten.tea.2013.0759 |
| format | Article |
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In this study, we report a method for human reconstructed skin (hRSK) when starting with human cells. We implanted tissue culture expanded human epidermal and dermal cells into an excision wound on the back of immunodeficient mice. Pigmented skin covered the wound 4 weeks after implantation. Hair shafts were visible at 12 weeks and prominent at 14 weeks. Histologically, the hRSK comprises an intact epidermis and dermis with mature hair follicles, sebaceous glands and most notably, and unique to this system, subcutis. Morphogenesis, differentiation, and maturation of the hRSK mirror the human fetal process. Human antigen markers demonstrate that the constituent cells are of human origin for at least 6 months. The degree of new skin formation is most complete when using tissue culture expanded cells from fetal skin, but it also occurs with expanded newborn and adult cells; however, no appendages formed when we grafted both adult dermal and epidermal cells. The hRSK system promises to be valuable as a laboratory model for studying biological, pathological, and pharmaceutical problems of human skin.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2013.0759</identifier><identifier>PMID: 25074625</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Cell culture ; Dermis - cytology ; Epidermis - cytology ; Hair Follicle - cytology ; Histology ; Humans ; Male ; Mice ; Original Articles ; Skin ; Skin - cytology ; Tissue Engineering</subject><ispartof>Tissue engineering. 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The degree of new skin formation is most complete when using tissue culture expanded cells from fetal skin, but it also occurs with expanded newborn and adult cells; however, no appendages formed when we grafted both adult dermal and epidermal cells. The hRSK system promises to be valuable as a laboratory model for studying biological, pathological, and pharmaceutical problems of human skin.</description><subject>Animals</subject><subject>Cell culture</subject><subject>Dermis - cytology</subject><subject>Epidermis - cytology</subject><subject>Hair Follicle - cytology</subject><subject>Histology</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Original Articles</subject><subject>Skin</subject><subject>Skin - cytology</subject><subject>Tissue Engineering</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkT1vFDEQhi0EIiHwA2iQJRqaPcYf648SnXI5pCAKDolu5dubVZx4vYe9VuDf4-VCCqoU1lieZ1559BDylsGKgbEfZ4yrGd2KAxMr0K19Rs6ZFboRov3x_PEu2Rl5lfMtgAKl9UtyxlvQUvH2nLhNCaHZ3fj-LmLO9Nudj_Tezzf0i5tLQrp1PtHNFILvA2Z6hRGTm_FAhzSNdOdzLkjXJfyFL38dXTzU5raMLtI1hpBfkxeDCxnfPNQL8n1zuVtvm-uvV5_Xn66bXrZmboa-1z3gwOpmDK2pKY4hKMWdkWxw1ri9EJYf5B5aDi2CHgzHgXMtrJVGXJAPp9xjmn4WzHM3-tzXH7iIU8kdU5IrrqSFJ6BCcm0qXtH3_6G3U0mxLrJQYKQCzSrFTlSfppwTDt0x-dGl3x2DblHVVVX1uG5R1S2q6sy7h-SyH_HwOPHPTQX0CVieXYzB4x7T_IToP8HHojc</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Wu, Xunwei</creator><creator>Scott, Larry</creator><creator>Washenik, Ken</creator><creator>Stenn, Kurt</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20141201</creationdate><title>Full-Thickness Skin with Mature Hair Follicles Generated from Tissue Culture Expanded Human Cells</title><author>Wu, Xunwei ; 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Xunwei</au><au>Scott, Larry</au><au>Washenik, Ken</au><au>Stenn, Kurt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Full-Thickness Skin with Mature Hair Follicles Generated from Tissue Culture Expanded Human Cells</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>20</volume><issue>23-24</issue><spage>3314</spage><epage>3321</epage><pages>3314-3321</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>The goal of regenerative medicine is to reconstruct fully functional organs from tissue culture expanded human cells. In this study, we report a method for human reconstructed skin (hRSK) when starting with human cells. 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| ispartof | Tissue engineering. Part A, 2014-12, Vol.20 (23-24), p.3314-3321 |
| issn | 1937-3341 1937-335X |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell culture Dermis - cytology Epidermis - cytology Hair Follicle - cytology Histology Humans Male Mice Original Articles Skin Skin - cytology Tissue Engineering |
| title | Full-Thickness Skin with Mature Hair Follicles Generated from Tissue Culture Expanded Human Cells |
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