Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique
INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hosp...
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Veröffentlicht in: | Revista da Sociedade Brasileira de Medicina Tropical 2012-03, Vol.45 (2), p.156-158 |
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description | INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emilio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 plus or minus 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm3 (average: 159 plus or minus 250 cells/mm3). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis. |
doi_str_mv | 10.1590/S0037-8682201200020000 |
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METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emilio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 plus or minus 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm3 (average: 159 plus or minus 250 cells/mm3). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis.</description><identifier>ISSN: 0037-8682</identifier><identifier>EISSN: 1678-9849</identifier><identifier>DOI: 10.1590/S0037-8682201200020000</identifier><language>eng</language><subject>Cryptosporidium ; Dientamoeba fragilis ; Endolimax nana ; Human immunodeficiency virus ; Isospora belli ; Schistosoma mansoni ; Strongyloides stercoralis</subject><ispartof>Revista da Sociedade Brasileira de Medicina Tropical, 2012-03, Vol.45 (2), p.156-158</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Garcia, Juliana Alves</creatorcontrib><title>Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique</title><title>Revista da Sociedade Brasileira de Medicina Tropical</title><description>INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emilio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 plus or minus 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm3 (average: 159 plus or minus 250 cells/mm3). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis.</description><subject>Cryptosporidium</subject><subject>Dientamoeba fragilis</subject><subject>Endolimax nana</subject><subject>Human immunodeficiency virus</subject><subject>Isospora belli</subject><subject>Schistosoma mansoni</subject><subject>Strongyloides stercoralis</subject><issn>0037-8682</issn><issn>1678-9849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqVjTtPw0AQhE-ISBjCX0Bb0pjcI_hRIgxK6iDa6BLW8aLznfGeBfn3XCSUnmI0xXwzI8Sdkg_qsZaLjZSmzKui0loqLaU8SV6ITBVlldfVsr4U2Zm5EtfMnwkqTa0z4RqMuI8UPIQWGkIfbR9wZ6Ed7YEcMZCHwcZTwvBNsYPV-n3xtG42sDvCxOQPYIGpHxy1hB9AYxrrsLcx_BxdaqeDztPXhHMxa61jvP3zG3H_-vL2vMqHMaSY47Yn3qNz1mOYeKuKpS60KY0y_0B_AQuBVIY</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Garcia, Juliana Alves</creator><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20120301</creationdate><title>Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique</title><author>Garcia, Juliana Alves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_16426237313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Cryptosporidium</topic><topic>Dientamoeba fragilis</topic><topic>Endolimax nana</topic><topic>Human immunodeficiency virus</topic><topic>Isospora belli</topic><topic>Schistosoma mansoni</topic><topic>Strongyloides stercoralis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcia, Juliana Alves</creatorcontrib><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Revista da Sociedade Brasileira de Medicina Tropical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcia, Juliana Alves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique</atitle><jtitle>Revista da Sociedade Brasileira de Medicina Tropical</jtitle><date>2012-03-01</date><risdate>2012</risdate><volume>45</volume><issue>2</issue><spage>156</spage><epage>158</epage><pages>156-158</pages><issn>0037-8682</issn><eissn>1678-9849</eissn><abstract>INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emilio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 plus or minus 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm3 (average: 159 plus or minus 250 cells/mm3). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis.</abstract><doi>10.1590/S0037-8682201200020000</doi></addata></record> |
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subjects | Cryptosporidium Dientamoeba fragilis Endolimax nana Human immunodeficiency virus Isospora belli Schistosoma mansoni Strongyloides stercoralis |
title | Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
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