MicroRNA-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting lipoprotein lipase gene in human THP-1 macrophages
Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by p...
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| Veröffentlicht in: | Biochimie 2014-11, Vol.106, p.81-90 |
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| creator | He, Ping-Ping Ouyang, Xin-Ping Tang, Yan-Yan Liao, Li Wang, Zong-Bao Lv, Yun-Cheng Tian, Guo-Ping Zhao, Guo-Jun Huang, Liang Yao, Feng Xie, Wei Tang, Yu Lin Chen, Wu-Jun Zhang, Min Li, Yuan Wu, Jian-Feng Peng, Juan Liu, Xiang-Yu Zheng, Xi-Long Yin, Wei-Dong Tang, Chao-Ke |
| description | Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting lipid accumulation and inflammatory response. However, the regulation of LPL at the post-transcriptional level by microRNAs has not been fully understood. In this study, we explored whether miR-590 affects the expression of LPL and its potential subsequent effects on lipid accumulation and pro-inflammatory cytokine secretion in human THP-1 macrophages.
Using bioinformatics analyses and dual-luciferase reporter assays, we found that miR-590 directly inhibited LPL protein and mRNA expression by targeting LPL 3′UTR. LPL Activity Assays showed that miR-590 reduced LPL activity in the culture media. Oil Red O staining and high-performance liquid chromatography assays showed that miR-590 had inhibitory effects on the lipid accumulation in human THP-1 macrophages. We also illustrated that miR-590 alleviated pro-inflammatory cytokine secretion in human THP-1 macrophages as measured by ELISA. With the method of small interfering RNA, we found that LPL siRNA can inhibit the miR-590 inhibitor-induced increase in lipid accumulation and secretion of pro-inflammatory cytokines in oxLDL-treated human THP-1 macrophages.
MiR-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting LPL gene in human THP-1 macrophages. Therefore, targeting miR-590 may offer a promising strategy to treat atherosclerotic cardiovascular diseases.
•Highly conserved miR-590 can directly target the 3′UTR of LPL.•MiR-590 can regulate the function of LPL.•MiR-590 attenuates lipid accumulation by targeting LPL gene in THP-1 macrophages.•MiR-590 attenuates inflammatory response by targeting LPL gene in THP-1 macrophages. |
| doi_str_mv | 10.1016/j.biochi.2014.08.003 |
| format | Article |
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Using bioinformatics analyses and dual-luciferase reporter assays, we found that miR-590 directly inhibited LPL protein and mRNA expression by targeting LPL 3′UTR. LPL Activity Assays showed that miR-590 reduced LPL activity in the culture media. Oil Red O staining and high-performance liquid chromatography assays showed that miR-590 had inhibitory effects on the lipid accumulation in human THP-1 macrophages. We also illustrated that miR-590 alleviated pro-inflammatory cytokine secretion in human THP-1 macrophages as measured by ELISA. With the method of small interfering RNA, we found that LPL siRNA can inhibit the miR-590 inhibitor-induced increase in lipid accumulation and secretion of pro-inflammatory cytokines in oxLDL-treated human THP-1 macrophages.
MiR-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting LPL gene in human THP-1 macrophages. Therefore, targeting miR-590 may offer a promising strategy to treat atherosclerotic cardiovascular diseases.
•Highly conserved miR-590 can directly target the 3′UTR of LPL.•MiR-590 can regulate the function of LPL.•MiR-590 attenuates lipid accumulation by targeting LPL gene in THP-1 macrophages.•MiR-590 attenuates inflammatory response by targeting LPL gene in THP-1 macrophages.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/j.biochi.2014.08.003</identifier><identifier>PMID: 25149060</identifier><language>eng</language><publisher>France: Elsevier B.V</publisher><subject>3' Untranslated Regions - genetics ; Atherosclerosis ; Base Sequence ; Blotting, Western ; Cell Line, Tumor ; Cytokines - secretion ; Gene Expression ; HEK293 Cells ; Human THP-1 macrophages ; Humans ; Inflammation Mediators - metabolism ; Lipids - analysis ; Lipoprotein Lipase - genetics ; Lipoprotein Lipase - metabolism ; Lipoproteins, LDL - pharmacology ; LPL ; Macrophages - drug effects ; Macrophages - metabolism ; MicroRNAs - genetics ; MiR-590 ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Interference ; Sequence Homology, Nucleic Acid</subject><ispartof>Biochimie, 2014-11, Vol.106, p.81-90</ispartof><rights>2014 Elsevier B.V. and Société française de biochimie et biologie Moléculaire (SFBBM)</rights><rights>Copyright © 2014 Elsevier B.V. and Société française de biochimie et biologie Moléculaire (SFBBM). All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c531t-83e1e04c8beb4c325791c7170da25ca32e3494f1275089dfc7f1dc36a410e4e23</citedby><cites>FETCH-LOGICAL-c531t-83e1e04c8beb4c325791c7170da25ca32e3494f1275089dfc7f1dc36a410e4e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0300908414002211$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25149060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Ping-Ping</creatorcontrib><creatorcontrib>Ouyang, Xin-Ping</creatorcontrib><creatorcontrib>Tang, Yan-Yan</creatorcontrib><creatorcontrib>Liao, Li</creatorcontrib><creatorcontrib>Wang, Zong-Bao</creatorcontrib><creatorcontrib>Lv, Yun-Cheng</creatorcontrib><creatorcontrib>Tian, Guo-Ping</creatorcontrib><creatorcontrib>Zhao, Guo-Jun</creatorcontrib><creatorcontrib>Huang, Liang</creatorcontrib><creatorcontrib>Yao, Feng</creatorcontrib><creatorcontrib>Xie, Wei</creatorcontrib><creatorcontrib>Tang, Yu Lin</creatorcontrib><creatorcontrib>Chen, Wu-Jun</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Wu, Jian-Feng</creatorcontrib><creatorcontrib>Peng, Juan</creatorcontrib><creatorcontrib>Liu, Xiang-Yu</creatorcontrib><creatorcontrib>Zheng, Xi-Long</creatorcontrib><creatorcontrib>Yin, Wei-Dong</creatorcontrib><creatorcontrib>Tang, Chao-Ke</creatorcontrib><title>MicroRNA-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting lipoprotein lipase gene in human THP-1 macrophages</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting lipid accumulation and inflammatory response. However, the regulation of LPL at the post-transcriptional level by microRNAs has not been fully understood. In this study, we explored whether miR-590 affects the expression of LPL and its potential subsequent effects on lipid accumulation and pro-inflammatory cytokine secretion in human THP-1 macrophages.
Using bioinformatics analyses and dual-luciferase reporter assays, we found that miR-590 directly inhibited LPL protein and mRNA expression by targeting LPL 3′UTR. LPL Activity Assays showed that miR-590 reduced LPL activity in the culture media. Oil Red O staining and high-performance liquid chromatography assays showed that miR-590 had inhibitory effects on the lipid accumulation in human THP-1 macrophages. We also illustrated that miR-590 alleviated pro-inflammatory cytokine secretion in human THP-1 macrophages as measured by ELISA. With the method of small interfering RNA, we found that LPL siRNA can inhibit the miR-590 inhibitor-induced increase in lipid accumulation and secretion of pro-inflammatory cytokines in oxLDL-treated human THP-1 macrophages.
MiR-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting LPL gene in human THP-1 macrophages. Therefore, targeting miR-590 may offer a promising strategy to treat atherosclerotic cardiovascular diseases.
•Highly conserved miR-590 can directly target the 3′UTR of LPL.•MiR-590 can regulate the function of LPL.•MiR-590 attenuates lipid accumulation by targeting LPL gene in THP-1 macrophages.•MiR-590 attenuates inflammatory response by targeting LPL gene in THP-1 macrophages.</description><subject>3' Untranslated Regions - genetics</subject><subject>Atherosclerosis</subject><subject>Base Sequence</subject><subject>Blotting, Western</subject><subject>Cell Line, Tumor</subject><subject>Cytokines - secretion</subject><subject>Gene Expression</subject><subject>HEK293 Cells</subject><subject>Human THP-1 macrophages</subject><subject>Humans</subject><subject>Inflammation Mediators - metabolism</subject><subject>Lipids - analysis</subject><subject>Lipoprotein Lipase - genetics</subject><subject>Lipoprotein Lipase - metabolism</subject><subject>Lipoproteins, LDL - pharmacology</subject><subject>LPL</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>MicroRNAs - genetics</subject><subject>MiR-590</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Interference</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdtu1DAQhi0EosvCGyDkS26Sjg85-AapquhBKrRC5dpynMmul8RZbAdpX4MnxssWLhFX9kjfPzP2R8hbBiUDVp_vys7NdutKDkyW0JYA4hlZsVq0Rc1a8ZysQAAUClp5Rl7FuAOACrh6Sc54xaSCGlbk5ydnw_zl80VRKaAmJfSLSRjp6Paup8baZVpGk9zsqfE93Ye5cH4YzTSZNIcDtYc0f3MeaUQb8DfXHWgyYZMLvzn2mXMoofPHu4lIN5jxXG6XyXj6ePNQMDqZvMZ-azYYX5MXgxkjvnk61-Tr1cfHy5vi7v769vLirrCVYKloBTIEadsOO2kFrxrFbMMa6A2vrBEchVRyYLypoFX9YJuB9VbURjJAiVysyftT37ze9wVj0pOLFsfReJyXqFktec0UE_-DcqWqtskK1kSe0PyeGAMOeh_cZMJBM9BHcXqnT-L0UZyGVudUjr17mrB0E_Z_Q39MZeDDCcD8JT8cBh2tQ2-xdwFt0v3s_j3hF1RQrM8</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>He, Ping-Ping</creator><creator>Ouyang, Xin-Ping</creator><creator>Tang, Yan-Yan</creator><creator>Liao, Li</creator><creator>Wang, Zong-Bao</creator><creator>Lv, Yun-Cheng</creator><creator>Tian, Guo-Ping</creator><creator>Zhao, Guo-Jun</creator><creator>Huang, Liang</creator><creator>Yao, Feng</creator><creator>Xie, Wei</creator><creator>Tang, Yu Lin</creator><creator>Chen, Wu-Jun</creator><creator>Zhang, Min</creator><creator>Li, Yuan</creator><creator>Wu, Jian-Feng</creator><creator>Peng, Juan</creator><creator>Liu, Xiang-Yu</creator><creator>Zheng, Xi-Long</creator><creator>Yin, Wei-Dong</creator><creator>Tang, Chao-Ke</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20141101</creationdate><title>MicroRNA-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting lipoprotein lipase gene in human THP-1 macrophages</title><author>He, Ping-Ping ; Ouyang, Xin-Ping ; Tang, Yan-Yan ; Liao, Li ; Wang, Zong-Bao ; Lv, Yun-Cheng ; Tian, Guo-Ping ; Zhao, Guo-Jun ; Huang, Liang ; Yao, Feng ; Xie, Wei ; Tang, Yu Lin ; Chen, Wu-Jun ; Zhang, Min ; Li, Yuan ; Wu, Jian-Feng ; Peng, Juan ; Liu, Xiang-Yu ; Zheng, Xi-Long ; Yin, Wei-Dong ; Tang, Chao-Ke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c531t-83e1e04c8beb4c325791c7170da25ca32e3494f1275089dfc7f1dc36a410e4e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>3' Untranslated Regions - genetics</topic><topic>Atherosclerosis</topic><topic>Base Sequence</topic><topic>Blotting, Western</topic><topic>Cell Line, Tumor</topic><topic>Cytokines - secretion</topic><topic>Gene Expression</topic><topic>HEK293 Cells</topic><topic>Human THP-1 macrophages</topic><topic>Humans</topic><topic>Inflammation Mediators - metabolism</topic><topic>Lipids - analysis</topic><topic>Lipoprotein Lipase - genetics</topic><topic>Lipoprotein Lipase - metabolism</topic><topic>Lipoproteins, LDL - pharmacology</topic><topic>LPL</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>MicroRNAs - genetics</topic><topic>MiR-590</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA Interference</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Ping-Ping</creatorcontrib><creatorcontrib>Ouyang, Xin-Ping</creatorcontrib><creatorcontrib>Tang, Yan-Yan</creatorcontrib><creatorcontrib>Liao, Li</creatorcontrib><creatorcontrib>Wang, Zong-Bao</creatorcontrib><creatorcontrib>Lv, Yun-Cheng</creatorcontrib><creatorcontrib>Tian, Guo-Ping</creatorcontrib><creatorcontrib>Zhao, Guo-Jun</creatorcontrib><creatorcontrib>Huang, Liang</creatorcontrib><creatorcontrib>Yao, Feng</creatorcontrib><creatorcontrib>Xie, Wei</creatorcontrib><creatorcontrib>Tang, Yu Lin</creatorcontrib><creatorcontrib>Chen, Wu-Jun</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Wu, Jian-Feng</creatorcontrib><creatorcontrib>Peng, Juan</creatorcontrib><creatorcontrib>Liu, Xiang-Yu</creatorcontrib><creatorcontrib>Zheng, Xi-Long</creatorcontrib><creatorcontrib>Yin, Wei-Dong</creatorcontrib><creatorcontrib>Tang, Chao-Ke</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Ping-Ping</au><au>Ouyang, Xin-Ping</au><au>Tang, Yan-Yan</au><au>Liao, Li</au><au>Wang, Zong-Bao</au><au>Lv, Yun-Cheng</au><au>Tian, Guo-Ping</au><au>Zhao, Guo-Jun</au><au>Huang, Liang</au><au>Yao, Feng</au><au>Xie, Wei</au><au>Tang, Yu Lin</au><au>Chen, Wu-Jun</au><au>Zhang, Min</au><au>Li, Yuan</au><au>Wu, Jian-Feng</au><au>Peng, Juan</au><au>Liu, Xiang-Yu</au><au>Zheng, Xi-Long</au><au>Yin, Wei-Dong</au><au>Tang, Chao-Ke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting lipoprotein lipase gene in human THP-1 macrophages</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>106</volume><spage>81</spage><epage>90</epage><pages>81-90</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><abstract>Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting lipid accumulation and inflammatory response. However, the regulation of LPL at the post-transcriptional level by microRNAs has not been fully understood. In this study, we explored whether miR-590 affects the expression of LPL and its potential subsequent effects on lipid accumulation and pro-inflammatory cytokine secretion in human THP-1 macrophages.
Using bioinformatics analyses and dual-luciferase reporter assays, we found that miR-590 directly inhibited LPL protein and mRNA expression by targeting LPL 3′UTR. LPL Activity Assays showed that miR-590 reduced LPL activity in the culture media. Oil Red O staining and high-performance liquid chromatography assays showed that miR-590 had inhibitory effects on the lipid accumulation in human THP-1 macrophages. We also illustrated that miR-590 alleviated pro-inflammatory cytokine secretion in human THP-1 macrophages as measured by ELISA. With the method of small interfering RNA, we found that LPL siRNA can inhibit the miR-590 inhibitor-induced increase in lipid accumulation and secretion of pro-inflammatory cytokines in oxLDL-treated human THP-1 macrophages.
MiR-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting LPL gene in human THP-1 macrophages. Therefore, targeting miR-590 may offer a promising strategy to treat atherosclerotic cardiovascular diseases.
•Highly conserved miR-590 can directly target the 3′UTR of LPL.•MiR-590 can regulate the function of LPL.•MiR-590 attenuates lipid accumulation by targeting LPL gene in THP-1 macrophages.•MiR-590 attenuates inflammatory response by targeting LPL gene in THP-1 macrophages.</abstract><cop>France</cop><pub>Elsevier B.V</pub><pmid>25149060</pmid><doi>10.1016/j.biochi.2014.08.003</doi><tpages>10</tpages></addata></record> |
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| subjects | 3' Untranslated Regions - genetics Atherosclerosis Base Sequence Blotting, Western Cell Line, Tumor Cytokines - secretion Gene Expression HEK293 Cells Human THP-1 macrophages Humans Inflammation Mediators - metabolism Lipids - analysis Lipoprotein Lipase - genetics Lipoprotein Lipase - metabolism Lipoproteins, LDL - pharmacology LPL Macrophages - drug effects Macrophages - metabolism MicroRNAs - genetics MiR-590 Reverse Transcriptase Polymerase Chain Reaction RNA Interference Sequence Homology, Nucleic Acid |
| title | MicroRNA-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting lipoprotein lipase gene in human THP-1 macrophages |
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