Exposure to DNA is insufficient for in vitro transgenesis of live bovine sperm and embryos

Transgenic mammals have been produced using sperm as vectors for exogenous DNA (sperm-mediated gene transfer (SMGT)) in combination with artificial insemination. Our study evaluated whether SMGT could also be achieved in combination with IVF to efficiently produce transgenic bovine embryos. We asses...

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Veröffentlicht in:	Reproduction (Cambridge, England) England), 2013-01, Vol.145 (1), p.97-108
Hauptverfasser:	Eghbalsaied, Shahin, Ghaedi, Kamran, Laible, Götz, Hosseini, Sayed Morteza, Forouzanfar, Mohsen, Hajian, Mehdi, Oback, Fleur, Nasr-Esfahani, Mohammad H, Oback, Björn
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified - genetics Cattle - genetics Cells, Cultured DNA - metabolism DNA - pharmacology Embryo, Mammalian - drug effects Embryo, Mammalian - metabolism Female Fertilization in Vitro - methods Gene Transfer Techniques Green Fluorescent Proteins - metabolism In Vitro Techniques Insemination, Artificial - methods Male Plasmids Sperm Injections, Intracytoplasmic - methods Spermatozoa - drug effects Spermatozoa - metabolism Transfection
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