Optimisation of Tetrahymena rostrata growth using food by-products as nitrogen source
The composition of media based on yeast extract and nitrogen sources for the growth of Tetrahymena rostrata was optimised by a central composite design. Dairy products promoted better growth than fish powder and starch products and improved generation time of 25% compared to previous reports. The be...
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| Veröffentlicht in: | Biotechnology techniques 1998-02, Vol.12 (2), p.177-181 |
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| container_title | Biotechnology techniques |
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| creator | CAILLEBRET-ETHUIN, P DUYME, F TONON, F JEANFILS, J DE CONINCK, J |
| description | The composition of media based on yeast extract and nitrogen sources for the growth of Tetrahymena rostrata was optimised by a central composite design. Dairy products promoted better growth than fish powder and starch products and improved generation time of 25% compared to previous reports. The best optimised combination was 5.7 g yeast extract/l + 6.6 g skimmed milk/l which gave the fastest generation time of 122 min and a maximal population of 8.4 X 10 super(5) cells/ml. In the case of buttermilk, two optimised combinations promoted growth: to induce fast growing (130 min) 2.5 g yeast extract/l + 4.4 g buttermilk/l must be chosen although to improve maximal population (9 X 10 super(5) cells/ml) 6 g yeast extract/l + 10 g buttermilk/l was more advisable. |
| doi_str_mv | 10.1023/a:1008857021199 |
| format | Article |
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Dairy products promoted better growth than fish powder and starch products and improved generation time of 25% compared to previous reports. The best optimised combination was 5.7 g yeast extract/l + 6.6 g skimmed milk/l which gave the fastest generation time of 122 min and a maximal population of 8.4 X 10 super(5) cells/ml. In the case of buttermilk, two optimised combinations promoted growth: to induce fast growing (130 min) 2.5 g yeast extract/l + 4.4 g buttermilk/l must be chosen although to improve maximal population (9 X 10 super(5) cells/ml) 6 g yeast extract/l + 10 g buttermilk/l was more advisable.</description><identifier>ISSN: 0951-208X</identifier><identifier>EISSN: 1573-6784</identifier><identifier>DOI: 10.1023/a:1008857021199</identifier><identifier>CODEN: BTECE6</identifier><language>eng</language><publisher>London: Chapman and Hall</publisher><subject>Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Methods. 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Dairy products promoted better growth than fish powder and starch products and improved generation time of 25% compared to previous reports. The best optimised combination was 5.7 g yeast extract/l + 6.6 g skimmed milk/l which gave the fastest generation time of 122 min and a maximal population of 8.4 X 10 super(5) cells/ml. In the case of buttermilk, two optimised combinations promoted growth: to induce fast growing (130 min) 2.5 g yeast extract/l + 4.4 g buttermilk/l must be chosen although to improve maximal population (9 X 10 super(5) cells/ml) 6 g yeast extract/l + 10 g buttermilk/l was more advisable.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. 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Fermentation and microbial culture technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CAILLEBRET-ETHUIN, P</creatorcontrib><creatorcontrib>DUYME, F</creatorcontrib><creatorcontrib>TONON, F</creatorcontrib><creatorcontrib>JEANFILS, J</creatorcontrib><creatorcontrib>DE CONINCK, J</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology techniques</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CAILLEBRET-ETHUIN, P</au><au>DUYME, F</au><au>TONON, F</au><au>JEANFILS, J</au><au>DE CONINCK, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimisation of Tetrahymena rostrata growth using food by-products as nitrogen source</atitle><jtitle>Biotechnology techniques</jtitle><date>1998-02-01</date><risdate>1998</risdate><volume>12</volume><issue>2</issue><spage>177</spage><epage>181</epage><pages>177-181</pages><issn>0951-208X</issn><eissn>1573-6784</eissn><coden>BTECE6</coden><abstract>The composition of media based on yeast extract and nitrogen sources for the growth of Tetrahymena rostrata was optimised by a central composite design. 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| language | eng |
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| source | SpringerLink Journals |
| subjects | Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology |
| title | Optimisation of Tetrahymena rostrata growth using food by-products as nitrogen source |
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