Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes
A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine–Hg2+–thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induce...
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| Veröffentlicht in: | Biosensors & bioelectronics 2014-11, Vol.61, p.14-20 |
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| creator | Zhu, Mengya Wang, Ying Deng, Yi Yao, Li B. Adeloju, Samuel Pan, Daodong Xue, Feng Wu, Yucheng Zheng, Lei Chen, Wei |
| description | A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine–Hg2+–thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.
•A one-step signal amplified lateral flow strip was developed for on-site detection of mercury ion.•Nucleic acid based signal amplification technique was successfully applied in lateral flow strip based methods.•Extraordinary sensitivity was realized for rapid and ultrasensitive detection of mercury ions.•A potential practical tool for food safety detection and environmental monitoring.•A generous signal amplification technique for nucleic acid probe based lateral flow strip. |
| doi_str_mv | 10.1016/j.bios.2014.04.049 |
| format | Article |
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•A one-step signal amplified lateral flow strip was developed for on-site detection of mercury ion.•Nucleic acid based signal amplification technique was successfully applied in lateral flow strip based methods.•Extraordinary sensitivity was realized for rapid and ultrasensitive detection of mercury ions.•A potential practical tool for food safety detection and environmental monitoring.•A generous signal amplification technique for nucleic acid probe based lateral flow strip.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.04.049</identifier><identifier>PMID: 24841089</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Amplification ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; DNA, Single-Stranded - chemistry ; Equipment Design ; Food industries ; Food safety ; Fundamental and applied biological sciences. Psychology ; General aspects ; Gold ; Gold - chemistry ; Gold nanoparticles ; Hg2+ detection ; Hygiene and safety ; Lateral flow strip ; Limit of Detection ; Marking ; Mercury (metal) ; Mercury - analysis ; Metal Nanoparticles - chemistry ; Probes ; Recognition ; Signal amplification ; Signal processing ; Strip ; Thymine - chemistry ; Water - analysis</subject><ispartof>Biosensors & bioelectronics, 2014-11, Vol.61, p.14-20</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-fcaf1bfdb2aff57978f6315a12cb4c89981d0e5893eaffb0bcc359bdb82fdf393</citedby><cites>FETCH-LOGICAL-c452t-fcaf1bfdb2aff57978f6315a12cb4c89981d0e5893eaffb0bcc359bdb82fdf393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2014.04.049$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28595929$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24841089$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Mengya</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Deng, Yi</creatorcontrib><creatorcontrib>Yao, Li</creatorcontrib><creatorcontrib>B. Adeloju, Samuel</creatorcontrib><creatorcontrib>Pan, Daodong</creatorcontrib><creatorcontrib>Xue, Feng</creatorcontrib><creatorcontrib>Wu, Yucheng</creatorcontrib><creatorcontrib>Zheng, Lei</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><title>Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine–Hg2+–thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.
•A one-step signal amplified lateral flow strip was developed for on-site detection of mercury ion.•Nucleic acid based signal amplification technique was successfully applied in lateral flow strip based methods.•Extraordinary sensitivity was realized for rapid and ultrasensitive detection of mercury ions.•A potential practical tool for food safety detection and environmental monitoring.•A generous signal amplification technique for nucleic acid probe based lateral flow strip.</description><subject>Amplification</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>Equipment Design</subject><subject>Food industries</subject><subject>Food safety</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Gold nanoparticles</subject><subject>Hg2+ detection</subject><subject>Hygiene and safety</subject><subject>Lateral flow strip</subject><subject>Limit of Detection</subject><subject>Marking</subject><subject>Mercury (metal)</subject><subject>Mercury - analysis</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Probes</subject><subject>Recognition</subject><subject>Signal amplification</subject><subject>Signal processing</subject><subject>Strip</subject><subject>Thymine - chemistry</subject><subject>Water - analysis</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcuKFDEUQAtRnHH0B1xINoKbavOoVFXAzTA-YdCNsy7yuOlJk0rKJN3DfJJ_acpudafChUDuuQ_uaZrnBG8IJv3r3Ua5mDcUk26D1xAPmnMyDqztKOMPm3MseN_yvmdnzZOcdxjjgQj8uDmj3dgRPIrz5vuNL0lmCNkVdwBkoIAuLgYULZoh6X26R3eu3CKJQjyARzFAmwssKLttkB7JefHOOjDIywKp_lgf71AuyS1I1damlqBt9AYFGeIiU3HaQ-ulAl-TOb_9fIkS6LgN7udkGQyCcCuDhhlCQUuKCvLT5pGVPsOz03vR3Lx_9_XqY3v95cOnq8vrVnecltZqaYmyRlFpLR_EMNqeES4J1arToxAjMRj4KBhUQGGlNeNCGTVSaywT7KJ5dexbx37bQy7T7LIG72WAuM8T6TtKBScd_w-UDqLHbGD_RnnH-kGwDleUHlGdYs4J7LQkN8t0PxE8reKn3bSKn1bxE15j3frFqf9ezWB-l_wyXYGXJ0BmLb1N9bwu_-FGLrigK_fmyEG98cFBmrJ2UFUYVx2VyUT3tz1-AE_P0JU</recordid><startdate>20141115</startdate><enddate>20141115</enddate><creator>Zhu, Mengya</creator><creator>Wang, Ying</creator><creator>Deng, Yi</creator><creator>Yao, Li</creator><creator>B. Adeloju, Samuel</creator><creator>Pan, Daodong</creator><creator>Xue, Feng</creator><creator>Wu, Yucheng</creator><creator>Zheng, Lei</creator><creator>Chen, Wei</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20141115</creationdate><title>Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes</title><author>Zhu, Mengya ; Wang, Ying ; Deng, Yi ; Yao, Li ; B. Adeloju, Samuel ; Pan, Daodong ; Xue, Feng ; Wu, Yucheng ; Zheng, Lei ; Chen, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-fcaf1bfdb2aff57978f6315a12cb4c89981d0e5893eaffb0bcc359bdb82fdf393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amplification</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>Equipment Design</topic><topic>Food industries</topic><topic>Food safety</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Gold nanoparticles</topic><topic>Hg2+ detection</topic><topic>Hygiene and safety</topic><topic>Lateral flow strip</topic><topic>Limit of Detection</topic><topic>Marking</topic><topic>Mercury (metal)</topic><topic>Mercury - analysis</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Probes</topic><topic>Recognition</topic><topic>Signal amplification</topic><topic>Signal processing</topic><topic>Strip</topic><topic>Thymine - chemistry</topic><topic>Water - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Mengya</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Deng, Yi</creatorcontrib><creatorcontrib>Yao, Li</creatorcontrib><creatorcontrib>B. 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Adeloju, Samuel</au><au>Pan, Daodong</au><au>Xue, Feng</au><au>Wu, Yucheng</au><au>Zheng, Lei</au><au>Chen, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2014-11-15</date><risdate>2014</risdate><volume>61</volume><spage>14</spage><epage>20</epage><pages>14-20</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine–Hg2+–thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.
•A one-step signal amplified lateral flow strip was developed for on-site detection of mercury ion.•Nucleic acid based signal amplification technique was successfully applied in lateral flow strip based methods.•Extraordinary sensitivity was realized for rapid and ultrasensitive detection of mercury ions.•A potential practical tool for food safety detection and environmental monitoring.•A generous signal amplification technique for nucleic acid probe based lateral flow strip.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>24841089</pmid><doi>10.1016/j.bios.2014.04.049</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2014-11, Vol.61, p.14-20 |
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| subjects | Amplification Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology DNA, Single-Stranded - chemistry Equipment Design Food industries Food safety Fundamental and applied biological sciences. Psychology General aspects Gold Gold - chemistry Gold nanoparticles Hg2+ detection Hygiene and safety Lateral flow strip Limit of Detection Marking Mercury (metal) Mercury - analysis Metal Nanoparticles - chemistry Probes Recognition Signal amplification Signal processing Strip Thymine - chemistry Water - analysis |
| title | Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes |
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