Indirectly suspended droplet microextraction of water-miscible organic solvents by salting-out effect for the determination of polycyclic aromatic hydrocarbons

A simple and low‐cost method that indirectly suspended droplet microextraction of water‐miscible organic solvents (ISDME) by salting‐out effect before high‐performance liquid chromatography and ultraviolet (HPLC‐UV) detection was used for the determination of polycyclic aromatic hydrocarbons (PAHs)...

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Veröffentlicht in:Environmental toxicology and chemistry 2014-12, Vol.33 (12), p.2694-2701
Hauptverfasser: Daneshfar, Ali, Khezeli, Tahere
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Khezeli, Tahere
description A simple and low‐cost method that indirectly suspended droplet microextraction of water‐miscible organic solvents (ISDME) by salting‐out effect before high‐performance liquid chromatography and ultraviolet (HPLC‐UV) detection was used for the determination of polycyclic aromatic hydrocarbons (PAHs) in different samples. The ISDME is a combination of salting‐out extraction of water‐miscible organic solvent and directly suspended droplet microextraction (DSDME). Ninety‐five microliters water‐miscible organic solvent (1‐propanol) was added to a 500‐µL sample. A homogeneous solution was formed immediately. To produce a steady vortex at the top of the solution, the sample was agitated at 700 rpm using a magnetic stirrer. By the addition of ammonium sulfate (saturated solution) to the homogeneous solution, 1‐propanol was separated and collected at the bottom of the steady vortex. Finally, 20 µL 1‐propanol was injected into HPLC‐UV. The effects of important parameters such as water‐miscible organic solvent (type and volume), type of salt, and extraction time were evaluated. Under optimum conditions, the method has a good linear calibration range (0.1 µg/L–300 µg/L), coefficients of determination (R2 > 0.998), low limits of detection (between 0.02 µg/L and 0.27 µg/L), and acceptable recovery (>85.0%). Environ Toxicol Chem 2014;33:2694–2701. © 2014 SETAC
doi_str_mv 10.1002/etc.2753
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The ISDME is a combination of salting‐out extraction of water‐miscible organic solvent and directly suspended droplet microextraction (DSDME). Ninety‐five microliters water‐miscible organic solvent (1‐propanol) was added to a 500‐µL sample. A homogeneous solution was formed immediately. To produce a steady vortex at the top of the solution, the sample was agitated at 700 rpm using a magnetic stirrer. By the addition of ammonium sulfate (saturated solution) to the homogeneous solution, 1‐propanol was separated and collected at the bottom of the steady vortex. Finally, 20 µL 1‐propanol was injected into HPLC‐UV. The effects of important parameters such as water‐miscible organic solvent (type and volume), type of salt, and extraction time were evaluated. Under optimum conditions, the method has a good linear calibration range (0.1 µg/L–300 µg/L), coefficients of determination (R2 &gt; 0.998), low limits of detection (between 0.02 µg/L and 0.27 µg/L), and acceptable recovery (&gt;85.0%). 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The ISDME is a combination of salting‐out extraction of water‐miscible organic solvent and directly suspended droplet microextraction (DSDME). Ninety‐five microliters water‐miscible organic solvent (1‐propanol) was added to a 500‐µL sample. A homogeneous solution was formed immediately. To produce a steady vortex at the top of the solution, the sample was agitated at 700 rpm using a magnetic stirrer. By the addition of ammonium sulfate (saturated solution) to the homogeneous solution, 1‐propanol was separated and collected at the bottom of the steady vortex. Finally, 20 µL 1‐propanol was injected into HPLC‐UV. The effects of important parameters such as water‐miscible organic solvent (type and volume), type of salt, and extraction time were evaluated. Under optimum conditions, the method has a good linear calibration range (0.1 µg/L–300 µg/L), coefficients of determination (R2 &gt; 0.998), low limits of detection (between 0.02 µg/L and 0.27 µg/L), and acceptable recovery (&gt;85.0%). 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purification</subject><subject>Polycyclic Aromatic Hydrocarbons - standards</subject><subject>Propanol</subject><subject>Salt</subject><subject>Salting</subject><subject>Salting-out extraction</subject><subject>Solution chemistry</subject><subject>Solvent extraction processes</subject><subject>Solvents</subject><subject>Solvents - chemistry</subject><subject>Spectrophotometry, Ultraviolet - standards</subject><subject>Vegetables - chemistry</subject><subject>Vortices</subject><subject>Water - chemistry</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water treatment and pollution</subject><issn>0730-7268</issn><issn>1552-8618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkttqFTEUhgdR7LYKPoEERPBmag4zk8yltFoLtXqhCN6ETLKmTc0ku0nGdp6mr9psutuCoHgVCN_6ftahql4SvEcwpu8g6z3KW_aoWpG2pbXoiHhcrTBnuOa0EzvVs5TOMSZd3_dPqx3a0oZS1q-q6yNvbASd3YLSnNbgDRhkYlg7yGiyOga4ylHpbINHYUSXKkOsJ5u0HRygEE-Vtxql4H6DzwkNxaNctv60DnNGMI5FjsYQUT4DZKBUT9arO906uEUv2hWFimEq_xqdLSVfqzgEn55XT0blErzYvrvV948fvu1_qo-_HB7tvz-uddsIVrOBg1GD0QIzpkxDO62AUa5I3_ERAyOd1qXlgdGGN0Ix0-BWgDJlfLqnmu1Wb2-96xguZkhZbloE55SHMCdJulItBCPsP1DWYsKJ4AV9_Qd6HuboSyOS4r7HLe85_hdVXF0jsCDkIbasJKUIo1xHO6m4SILl5gpkuQK5uYKCvtoK52ECcw_erb0Ab7aASlq5MSqvbXrgRN9RRjdcfctdWgfLXwNlYbbBW96mDFf3vIq_ZMcZb-WPk0P59eBz85P3J_KA3QBhddms</recordid><startdate>201412</startdate><enddate>201412</enddate><creator>Daneshfar, Ali</creator><creator>Khezeli, Tahere</creator><general>Blackwell Publishing Ltd</general><general>SETAC</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7SU</scope><scope>7U5</scope><scope>KR7</scope><scope>L7M</scope></search><sort><creationdate>201412</creationdate><title>Indirectly suspended droplet microextraction of water-miscible organic solvents by salting-out effect for the determination of polycyclic aromatic hydrocarbons</title><author>Daneshfar, Ali ; Khezeli, Tahere</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5483-3b7edabdc8033ad426cae327a1967f0e316cc422b324748a3d4058ead100c92c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>1-Propanol - chemistry</topic><topic>Acceptability</topic><topic>Ammonium</topic><topic>Ammonium sulfate</topic><topic>Ammonium Sulfate - chemistry</topic><topic>Analysis methods</topic><topic>Analytical chemistry</topic><topic>Applied sciences</topic><topic>Calibration</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Droplets</topic><topic>Exact sciences and technology</topic><topic>Extraction</topic><topic>Extraction processes</topic><topic>Fluid flow</topic><topic>Fruit - chemistry</topic><topic>High performance liquid chromatography</topic><topic>high-performance liquid chromatography and ultraviolet detection (HPLC-UV)</topic><topic>Indirectly suspended droplet</topic><topic>Liquid chromatography</topic><topic>Liquid Phase Microextraction</topic><topic>Miscibility</topic><topic>Natural water pollution</topic><topic>Organic chemistry</topic><topic>Organic solvents</topic><topic>Other chromatographic methods</topic><topic>Pollution</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>polycyclic aromatic hydrocarbons (PAHs)</topic><topic>Polycyclic Aromatic Hydrocarbons - analysis</topic><topic>Polycyclic Aromatic Hydrocarbons - isolation &amp; purification</topic><topic>Polycyclic Aromatic Hydrocarbons - standards</topic><topic>Propanol</topic><topic>Salt</topic><topic>Salting</topic><topic>Salting-out extraction</topic><topic>Solution chemistry</topic><topic>Solvent extraction processes</topic><topic>Solvents</topic><topic>Solvents - chemistry</topic><topic>Spectrophotometry, Ultraviolet - standards</topic><topic>Vegetables - chemistry</topic><topic>Vortices</topic><topic>Water - chemistry</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Daneshfar, Ali</creatorcontrib><creatorcontrib>Khezeli, Tahere</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health &amp; 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The ISDME is a combination of salting‐out extraction of water‐miscible organic solvent and directly suspended droplet microextraction (DSDME). Ninety‐five microliters water‐miscible organic solvent (1‐propanol) was added to a 500‐µL sample. A homogeneous solution was formed immediately. To produce a steady vortex at the top of the solution, the sample was agitated at 700 rpm using a magnetic stirrer. By the addition of ammonium sulfate (saturated solution) to the homogeneous solution, 1‐propanol was separated and collected at the bottom of the steady vortex. Finally, 20 µL 1‐propanol was injected into HPLC‐UV. The effects of important parameters such as water‐miscible organic solvent (type and volume), type of salt, and extraction time were evaluated. Under optimum conditions, the method has a good linear calibration range (0.1 µg/L–300 µg/L), coefficients of determination (R2 &gt; 0.998), low limits of detection (between 0.02 µg/L and 0.27 µg/L), and acceptable recovery (&gt;85.0%). Environ Toxicol Chem 2014;33:2694–2701. © 2014 SETAC</abstract><cop>Pensacola, FL</cop><pub>Blackwell Publishing Ltd</pub><pmid>25242239</pmid><doi>10.1002/etc.2753</doi><tpages>8</tpages></addata></record>
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects 1-Propanol - chemistry
Acceptability
Ammonium
Ammonium sulfate
Ammonium Sulfate - chemistry
Analysis methods
Analytical chemistry
Applied sciences
Calibration
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Chromatography, High Pressure Liquid
Droplets
Exact sciences and technology
Extraction
Extraction processes
Fluid flow
Fruit - chemistry
High performance liquid chromatography
high-performance liquid chromatography and ultraviolet detection (HPLC-UV)
Indirectly suspended droplet
Liquid chromatography
Liquid Phase Microextraction
Miscibility
Natural water pollution
Organic chemistry
Organic solvents
Other chromatographic methods
Pollution
Polycyclic aromatic hydrocarbons
polycyclic aromatic hydrocarbons (PAHs)
Polycyclic Aromatic Hydrocarbons - analysis
Polycyclic Aromatic Hydrocarbons - isolation & purification
Polycyclic Aromatic Hydrocarbons - standards
Propanol
Salt
Salting
Salting-out extraction
Solution chemistry
Solvent extraction processes
Solvents
Solvents - chemistry
Spectrophotometry, Ultraviolet - standards
Vegetables - chemistry
Vortices
Water - chemistry
Water Pollutants, Chemical - analysis
Water treatment and pollution
title Indirectly suspended droplet microextraction of water-miscible organic solvents by salting-out effect for the determination of polycyclic aromatic hydrocarbons
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