Biochemical features and bioethanol production of microalgae from coastal waters of Pearl River Delta
► Two native to Pearl River Delta algae were used for bioethanol production. ► Both strains exhibited highest growth rates in aerated cultures. ► Accumulation of cellular carbohydrates was highest in early stationary phase. ► Highest bioethanol yield from S. abundans was obtained after two step hydr...
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| creator | Guo, Hui Daroch, Maurycy Liu, Lei Qiu, Guoyu Geng, Shu Wang, Guangyi |
| description | ► Two native to Pearl River Delta algae were used for bioethanol production. ► Both strains exhibited highest growth rates in aerated cultures. ► Accumulation of cellular carbohydrates was highest in early stationary phase. ► Highest bioethanol yield from S. abundans was obtained after two step hydrolysis.
This study describes identification, cultivation, monitoring of carbohydrate accumulation and bioethanol production from microalgal strains from the coastal waters of Pearl River Delta. Eighteen identified strains belong to the families Chlorellaceae, Scotiellocystoidaceae, Neochloridaceae, Selenastraceae and Scenedesmaceae. Of isolated strains Mychonastes afer PKUAC 9 and Scenedesmus abundans PKUAC 12 were selected for further biomass and ethanol production analysis. Comparison of three cultivation modes (stationary, shaken and aerated) resulted in the highest biomass productivity obtained for aerated cultures that yielded 0.09g and 0.11g dry weight per day per litre of medium for M. afer PKUAC 9 and S. abundans PKUAC 12, respectively. Carbohydrate accumulation monitored by FTIR showed that early stationary phase is optimal for biomass harvest. Microalgal biomass was successfully used as a carbohydrate feedstock for fermentative bioethanol production. S. abundans PKUAC 12 was superior feedstock for bioethanol production when pre-treated with the combination of dilute acid treatment and cellulase. |
| doi_str_mv | 10.1016/j.biortech.2012.10.006 |
| format | Article |
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This study describes identification, cultivation, monitoring of carbohydrate accumulation and bioethanol production from microalgal strains from the coastal waters of Pearl River Delta. Eighteen identified strains belong to the families Chlorellaceae, Scotiellocystoidaceae, Neochloridaceae, Selenastraceae and Scenedesmaceae. Of isolated strains Mychonastes afer PKUAC 9 and Scenedesmus abundans PKUAC 12 were selected for further biomass and ethanol production analysis. Comparison of three cultivation modes (stationary, shaken and aerated) resulted in the highest biomass productivity obtained for aerated cultures that yielded 0.09g and 0.11g dry weight per day per litre of medium for M. afer PKUAC 9 and S. abundans PKUAC 12, respectively. Carbohydrate accumulation monitored by FTIR showed that early stationary phase is optimal for biomass harvest. Microalgal biomass was successfully used as a carbohydrate feedstock for fermentative bioethanol production. S. abundans PKUAC 12 was superior feedstock for bioethanol production when pre-treated with the combination of dilute acid treatment and cellulase.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2012.10.006</identifier><identifier>PMID: 23138065</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>acid treatment ; Algal bioethanol ; Amylases - metabolism ; Base Sequence ; Biofuel production ; Biofuels ; Biological and medical sciences ; Biomass ; biomass production ; Biotechnology ; Brackish ; Carbohydrate accumulation ; Carbohydrates ; Carbohydrates - biosynthesis ; Cell Culture Techniques - methods ; Cellulase - metabolism ; China ; Chlorellaceae ; Coastal water ; Deltas ; DNA Primers - genetics ; endo-1,4-beta-glucanase ; Energy ; Ethanol - metabolism ; ethanol production ; Ethyl alcohol ; Feedstock ; feedstocks ; Fourier transform infrared spectroscopy ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; Industrial applications and implications. Economical aspects ; Likelihood Functions ; Methods. Procedures. Technologies ; microalgae ; Microalgae - genetics ; Microalgae - metabolism ; Microalgae cultivation ; Microalgae diversity ; Microbial engineering. Fermentation and microbial culture technology ; Models, Genetic ; Molecular Sequence Data ; monitoring ; Neochloridaceae ; Phylogeny ; Polymorphism, Restriction Fragment Length ; river water ; Rivers ; Rivers - microbiology ; RNA, Ribosomal, 18S ; Scenedesmaceae ; Scenedesmus ; Scenedesmus - genetics ; Scenedesmus - metabolism ; Scenedesmus abundans ; Sequence Analysis, DNA ; Spectroscopy, Fourier Transform Infrared ; Strain</subject><ispartof>Bioresource technology, 2013-01, Vol.127, p.422-428</ispartof><rights>2012 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-30eafd83537139257294e5a2f7adceb2518dcb4ffb7fd69cbf232b6393c280f63</citedby><cites>FETCH-LOGICAL-c488t-30eafd83537139257294e5a2f7adceb2518dcb4ffb7fd69cbf232b6393c280f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852412015088$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27081529$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23138065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Hui</creatorcontrib><creatorcontrib>Daroch, Maurycy</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Qiu, Guoyu</creatorcontrib><creatorcontrib>Geng, Shu</creatorcontrib><creatorcontrib>Wang, Guangyi</creatorcontrib><title>Biochemical features and bioethanol production of microalgae from coastal waters of Pearl River Delta</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>► Two native to Pearl River Delta algae were used for bioethanol production. ► Both strains exhibited highest growth rates in aerated cultures. ► Accumulation of cellular carbohydrates was highest in early stationary phase. ► Highest bioethanol yield from S. abundans was obtained after two step hydrolysis.
This study describes identification, cultivation, monitoring of carbohydrate accumulation and bioethanol production from microalgal strains from the coastal waters of Pearl River Delta. Eighteen identified strains belong to the families Chlorellaceae, Scotiellocystoidaceae, Neochloridaceae, Selenastraceae and Scenedesmaceae. Of isolated strains Mychonastes afer PKUAC 9 and Scenedesmus abundans PKUAC 12 were selected for further biomass and ethanol production analysis. Comparison of three cultivation modes (stationary, shaken and aerated) resulted in the highest biomass productivity obtained for aerated cultures that yielded 0.09g and 0.11g dry weight per day per litre of medium for M. afer PKUAC 9 and S. abundans PKUAC 12, respectively. Carbohydrate accumulation monitored by FTIR showed that early stationary phase is optimal for biomass harvest. Microalgal biomass was successfully used as a carbohydrate feedstock for fermentative bioethanol production. S. abundans PKUAC 12 was superior feedstock for bioethanol production when pre-treated with the combination of dilute acid treatment and cellulase.</description><subject>acid treatment</subject><subject>Algal bioethanol</subject><subject>Amylases - metabolism</subject><subject>Base Sequence</subject><subject>Biofuel production</subject><subject>Biofuels</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>biomass production</subject><subject>Biotechnology</subject><subject>Brackish</subject><subject>Carbohydrate accumulation</subject><subject>Carbohydrates</subject><subject>Carbohydrates - biosynthesis</subject><subject>Cell Culture Techniques - methods</subject><subject>Cellulase - metabolism</subject><subject>China</subject><subject>Chlorellaceae</subject><subject>Coastal water</subject><subject>Deltas</subject><subject>DNA Primers - genetics</subject><subject>endo-1,4-beta-glucanase</subject><subject>Energy</subject><subject>Ethanol - metabolism</subject><subject>ethanol production</subject><subject>Ethyl alcohol</subject><subject>Feedstock</subject><subject>feedstocks</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Likelihood Functions</subject><subject>Methods. Procedures. Technologies</subject><subject>microalgae</subject><subject>Microalgae - genetics</subject><subject>Microalgae - metabolism</subject><subject>Microalgae cultivation</subject><subject>Microalgae diversity</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>monitoring</subject><subject>Neochloridaceae</subject><subject>Phylogeny</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>river water</subject><subject>Rivers</subject><subject>Rivers - microbiology</subject><subject>RNA, Ribosomal, 18S</subject><subject>Scenedesmaceae</subject><subject>Scenedesmus</subject><subject>Scenedesmus - genetics</subject><subject>Scenedesmus - metabolism</subject><subject>Scenedesmus abundans</subject><subject>Sequence Analysis, DNA</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Strain</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhLxRfkLhkGduJ7dyg5VOqBAJ6thxn3PUqiYudFPHv8SpbOK4vlkbPOzP2Q8gFgy0DJt_st12IaUa323JgvBS3APIR2TCtRMVbJR-TDbQSKt3w-ow8y3kPAIIp_pScccGEBtlsCF6G6HY4BmcH6tHOS8JM7dTT0h_nnZ3iQO9S7Bc3hzjR6GlhU7TDrUXqUxypizbPJf3bzpjygfiGNg30e7jHRN_jMNvn5Im3Q8YXx_uc3Hz88PPqc3X99dOXq3fXlau1nisBaH2vRSMUEy1vFG9rbCz3yvYOO94w3buu9r5Tvpet6zwXvJOiFY5r8FKck9dr37LxrwXzbMaQHQ6DnTAu2TBZcy6FZvVptAFQqtagTqO8HAF1rQsqV7R8Uc4JvblLYbTpj2FgDuLM3jyIMwdxh3oRV4IXxxlLN2L_L_ZgqgCvjoDNxZVPdnIh_-cUaNbwtnAvV87baOxtKszNjzKpBmBarM95uxJYTNwHTCa7gJPDPiR0s-ljOLXtX2BCwwE</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Guo, Hui</creator><creator>Daroch, Maurycy</creator><creator>Liu, Lei</creator><creator>Qiu, Guoyu</creator><creator>Geng, Shu</creator><creator>Wang, Guangyi</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7SU</scope><scope>7TB</scope><scope>C1K</scope><scope>KR7</scope></search><sort><creationdate>201301</creationdate><title>Biochemical features and bioethanol production of microalgae from coastal waters of Pearl River Delta</title><author>Guo, Hui ; Daroch, Maurycy ; Liu, Lei ; Qiu, Guoyu ; Geng, Shu ; Wang, Guangyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-30eafd83537139257294e5a2f7adceb2518dcb4ffb7fd69cbf232b6393c280f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>acid treatment</topic><topic>Algal bioethanol</topic><topic>Amylases - metabolism</topic><topic>Base Sequence</topic><topic>Biofuel production</topic><topic>Biofuels</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>biomass production</topic><topic>Biotechnology</topic><topic>Brackish</topic><topic>Carbohydrate accumulation</topic><topic>Carbohydrates</topic><topic>Carbohydrates - biosynthesis</topic><topic>Cell Culture Techniques - methods</topic><topic>Cellulase - metabolism</topic><topic>China</topic><topic>Chlorellaceae</topic><topic>Coastal water</topic><topic>Deltas</topic><topic>DNA Primers - genetics</topic><topic>endo-1,4-beta-glucanase</topic><topic>Energy</topic><topic>Ethanol - metabolism</topic><topic>ethanol production</topic><topic>Ethyl alcohol</topic><topic>Feedstock</topic><topic>feedstocks</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Likelihood Functions</topic><topic>Methods. Procedures. Technologies</topic><topic>microalgae</topic><topic>Microalgae - genetics</topic><topic>Microalgae - metabolism</topic><topic>Microalgae cultivation</topic><topic>Microalgae diversity</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>monitoring</topic><topic>Neochloridaceae</topic><topic>Phylogeny</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>river water</topic><topic>Rivers</topic><topic>Rivers - microbiology</topic><topic>RNA, Ribosomal, 18S</topic><topic>Scenedesmaceae</topic><topic>Scenedesmus</topic><topic>Scenedesmus - genetics</topic><topic>Scenedesmus - metabolism</topic><topic>Scenedesmus abundans</topic><topic>Sequence Analysis, DNA</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Strain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Hui</creatorcontrib><creatorcontrib>Daroch, Maurycy</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Qiu, Guoyu</creatorcontrib><creatorcontrib>Geng, Shu</creatorcontrib><creatorcontrib>Wang, Guangyi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Hui</au><au>Daroch, Maurycy</au><au>Liu, Lei</au><au>Qiu, Guoyu</au><au>Geng, Shu</au><au>Wang, Guangyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical features and bioethanol production of microalgae from coastal waters of Pearl River Delta</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2013-01</date><risdate>2013</risdate><volume>127</volume><spage>422</spage><epage>428</epage><pages>422-428</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>► Two native to Pearl River Delta algae were used for bioethanol production. ► Both strains exhibited highest growth rates in aerated cultures. ► Accumulation of cellular carbohydrates was highest in early stationary phase. ► Highest bioethanol yield from S. abundans was obtained after two step hydrolysis.
This study describes identification, cultivation, monitoring of carbohydrate accumulation and bioethanol production from microalgal strains from the coastal waters of Pearl River Delta. Eighteen identified strains belong to the families Chlorellaceae, Scotiellocystoidaceae, Neochloridaceae, Selenastraceae and Scenedesmaceae. Of isolated strains Mychonastes afer PKUAC 9 and Scenedesmus abundans PKUAC 12 were selected for further biomass and ethanol production analysis. Comparison of three cultivation modes (stationary, shaken and aerated) resulted in the highest biomass productivity obtained for aerated cultures that yielded 0.09g and 0.11g dry weight per day per litre of medium for M. afer PKUAC 9 and S. abundans PKUAC 12, respectively. Carbohydrate accumulation monitored by FTIR showed that early stationary phase is optimal for biomass harvest. Microalgal biomass was successfully used as a carbohydrate feedstock for fermentative bioethanol production. S. abundans PKUAC 12 was superior feedstock for bioethanol production when pre-treated with the combination of dilute acid treatment and cellulase.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23138065</pmid><doi>10.1016/j.biortech.2012.10.006</doi><tpages>7</tpages></addata></record> |
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| subjects | acid treatment Algal bioethanol Amylases - metabolism Base Sequence Biofuel production Biofuels Biological and medical sciences Biomass biomass production Biotechnology Brackish Carbohydrate accumulation Carbohydrates Carbohydrates - biosynthesis Cell Culture Techniques - methods Cellulase - metabolism China Chlorellaceae Coastal water Deltas DNA Primers - genetics endo-1,4-beta-glucanase Energy Ethanol - metabolism ethanol production Ethyl alcohol Feedstock feedstocks Fourier transform infrared spectroscopy Freshwater Fundamental and applied biological sciences. Psychology Hydrolysis Industrial applications and implications. Economical aspects Likelihood Functions Methods. Procedures. Technologies microalgae Microalgae - genetics Microalgae - metabolism Microalgae cultivation Microalgae diversity Microbial engineering. Fermentation and microbial culture technology Models, Genetic Molecular Sequence Data monitoring Neochloridaceae Phylogeny Polymorphism, Restriction Fragment Length river water Rivers Rivers - microbiology RNA, Ribosomal, 18S Scenedesmaceae Scenedesmus Scenedesmus - genetics Scenedesmus - metabolism Scenedesmus abundans Sequence Analysis, DNA Spectroscopy, Fourier Transform Infrared Strain |
| title | Biochemical features and bioethanol production of microalgae from coastal waters of Pearl River Delta |
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