Preinitiation complex assembly: potentially a bumpy path

Preinitiation complex assembly: potentially a bumpy path

During 1996 and 1997, several chemical issues that arise in the early stages of preinitiation complex (PIC) formation were resolved. Kinetics experiments indicated that both TBP dimerization and DNA bending influence the rate of TBP—TATA box assembly. Affinity cleavage experiments indicated that TBP...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Current opinion in chemical biology 1998-02, Vol.2 (1), p.11-17
Hauptverfasser: Cox, Julia M, Kays, Alexis R, Sanchez, Jennifer F, Schepartz, Alanna
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:During 1996 and 1997, several chemical issues that arise in the early stages of preinitiation complex (PIC) formation were resolved. Kinetics experiments indicated that both TBP dimerization and DNA bending influence the rate of TBP—TATA box assembly. Affinity cleavage experiments indicated that TBP lacks the specificity to nucleate assembly of a properly oriented PIC. Finally, high-resolution structures provided the atomic detail of early intermediates in PIC formation.
ISSN:1367-5931
1879-0402
DOI:10.1016/S1367-5931(98)80030-6