In vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm laser and malachite green dye in Staphylococcus aureus biofilms arranged on compact and cancellous bone specimens
The aim of this study was to evaluate the in vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm visible laser combined with malachite green (MG) dye in the inactivation of Staphylococcus aureus (ATCC 25923) biofilms formed within compact and cancellous bone specimens. Sp...
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| Veröffentlicht in: | Lasers in medical science 2014-11, Vol.29 (6), p.1959-1965 |
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| container_end_page | 1965 |
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| container_issue | 6 |
| container_start_page | 1959 |
| container_title | Lasers in medical science |
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| creator | Rosa, Luciano Pereira da Silva, Francine Cristina Nader, Sumaia Alves Meira, Giselle Andrade Viana, Magda Souza |
| description | The aim of this study was to evaluate the in vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm visible laser combined with malachite green (MG) dye in the inactivation of
Staphylococcus aureus
(ATCC 25923) biofilms formed within compact and cancellous bone specimens. Specimens of 80 compact bones and 80 cancellous bones were contaminated with a standard suspension of
S. aureus
and incubated for 14 days at 37 °C to allow for the formation of biofilms. The specimens were divided into the following groups (
n
= 10) according to the treatment conditions: PS−L − (control — no treatment), PS+L − (only MG for 5 min), PS−L + 90 (only laser irradiation for 90 s), PS−L + 180 (only laser irradiation for 180 s), PS−L + 300 (only laser irradiation for 300 s), APDT90 (APDT for 90 s), APDT180 (APDT for 180 s), and APDT300 (APDT for 300 s). The findings were statistically analyzed using an ANOVA 5 %. All of the experimental groups were significantly different from the control group for both the compact and cancellous bone specimens. The compact bone specimens that received APDT treatment (for either 90, 180, or 300 s) showed reductions in the log10 CFU/ml of
S. aureus
by a magnitude of 4 log10. Cancellous bone specimens treated with 300 s of APDT showed the highest efficacy, and these specimens had a reduction in
S. aureus
CFU/ml by a factor of 3 log10. APDT treatment using these proposed parameters in combination with MG was effective at inactivating
S. aureus
biofilms in compact and cancellous bone specimens. |
| doi_str_mv | 10.1007/s10103-014-1613-5 |
| format | Article |
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Staphylococcus aureus
(ATCC 25923) biofilms formed within compact and cancellous bone specimens. Specimens of 80 compact bones and 80 cancellous bones were contaminated with a standard suspension of
S. aureus
and incubated for 14 days at 37 °C to allow for the formation of biofilms. The specimens were divided into the following groups (
n
= 10) according to the treatment conditions: PS−L − (control — no treatment), PS+L − (only MG for 5 min), PS−L + 90 (only laser irradiation for 90 s), PS−L + 180 (only laser irradiation for 180 s), PS−L + 300 (only laser irradiation for 300 s), APDT90 (APDT for 90 s), APDT180 (APDT for 180 s), and APDT300 (APDT for 300 s). The findings were statistically analyzed using an ANOVA 5 %. All of the experimental groups were significantly different from the control group for both the compact and cancellous bone specimens. The compact bone specimens that received APDT treatment (for either 90, 180, or 300 s) showed reductions in the log10 CFU/ml of
S. aureus
by a magnitude of 4 log10. Cancellous bone specimens treated with 300 s of APDT showed the highest efficacy, and these specimens had a reduction in
S. aureus
CFU/ml by a factor of 3 log10. APDT treatment using these proposed parameters in combination with MG was effective at inactivating
S. aureus
biofilms in compact and cancellous bone specimens.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-014-1613-5</identifier><identifier>PMID: 24935702</identifier><identifier>CODEN: LMSCEZ</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Analysis of variance ; Animals ; Anti-Infective Agents - chemistry ; Biofilms ; Biofilms - radiation effects ; Bone and Bones - drug effects ; Bone and Bones - microbiology ; Bone and Bones - radiation effects ; Bones ; Cattle ; Coloring Agents - chemistry ; Dentistry ; Effectiveness studies ; In vitro testing ; Irradiation ; Laser surgery ; Laser Therapy - methods ; Lasers ; Medicine ; Medicine & Public Health ; Optical Devices ; Optics ; Original Article ; Photochemotherapy - methods ; Photonics ; Quantum Optics ; Reduction ; Rosaniline Dyes - chemistry ; Staphylococcus aureus ; Staphylococcus aureus - radiation effects ; Therapy ; Tibia - drug effects ; Tibia - microbiology ; Tibia - radiation effects</subject><ispartof>Lasers in medical science, 2014-11, Vol.29 (6), p.1959-1965</ispartof><rights>Springer-Verlag London 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-4725e46952dacad4e98123b3c22430abf9c5b6e5cc5340bce7f37c9c9b45f363</citedby><cites>FETCH-LOGICAL-c508t-4725e46952dacad4e98123b3c22430abf9c5b6e5cc5340bce7f37c9c9b45f363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-014-1613-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-014-1613-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24935702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosa, Luciano Pereira</creatorcontrib><creatorcontrib>da Silva, Francine Cristina</creatorcontrib><creatorcontrib>Nader, Sumaia Alves</creatorcontrib><creatorcontrib>Meira, Giselle Andrade</creatorcontrib><creatorcontrib>Viana, Magda Souza</creatorcontrib><title>In vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm laser and malachite green dye in Staphylococcus aureus biofilms arranged on compact and cancellous bone specimens</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>The aim of this study was to evaluate the in vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm visible laser combined with malachite green (MG) dye in the inactivation of
Staphylococcus aureus
(ATCC 25923) biofilms formed within compact and cancellous bone specimens. Specimens of 80 compact bones and 80 cancellous bones were contaminated with a standard suspension of
S. aureus
and incubated for 14 days at 37 °C to allow for the formation of biofilms. The specimens were divided into the following groups (
n
= 10) according to the treatment conditions: PS−L − (control — no treatment), PS+L − (only MG for 5 min), PS−L + 90 (only laser irradiation for 90 s), PS−L + 180 (only laser irradiation for 180 s), PS−L + 300 (only laser irradiation for 300 s), APDT90 (APDT for 90 s), APDT180 (APDT for 180 s), and APDT300 (APDT for 300 s). The findings were statistically analyzed using an ANOVA 5 %. All of the experimental groups were significantly different from the control group for both the compact and cancellous bone specimens. The compact bone specimens that received APDT treatment (for either 90, 180, or 300 s) showed reductions in the log10 CFU/ml of
S. aureus
by a magnitude of 4 log10. Cancellous bone specimens treated with 300 s of APDT showed the highest efficacy, and these specimens had a reduction in
S. aureus
CFU/ml by a factor of 3 log10. APDT treatment using these proposed parameters in combination with MG was effective at inactivating
S. aureus
biofilms in compact and cancellous bone specimens.</description><subject>Analysis of variance</subject><subject>Animals</subject><subject>Anti-Infective Agents - chemistry</subject><subject>Biofilms</subject><subject>Biofilms - radiation effects</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - microbiology</subject><subject>Bone and Bones - radiation effects</subject><subject>Bones</subject><subject>Cattle</subject><subject>Coloring Agents - chemistry</subject><subject>Dentistry</subject><subject>Effectiveness studies</subject><subject>In vitro testing</subject><subject>Irradiation</subject><subject>Laser surgery</subject><subject>Laser Therapy - methods</subject><subject>Lasers</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photochemotherapy - methods</subject><subject>Photonics</subject><subject>Quantum Optics</subject><subject>Reduction</subject><subject>Rosaniline Dyes - chemistry</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - radiation effects</subject><subject>Therapy</subject><subject>Tibia - drug effects</subject><subject>Tibia - microbiology</subject><subject>Tibia - radiation effects</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks-K1TAUxosoznX0AdxIwM24qOZ_2-Uw45-BAQXvwl1JT0_vzdAmNUkH7tv4LILvZeodRQRhVockv-87J5yvKJ4z-ppRWr2JjDIqSspkyTQTpXpQbJgUqtRUfnlYbCjXdVk3nJ0UT2K8oZRVGXtcnHDZCFVRvil-XDlya1PwBIcBIdlbdBgj8QMxLtnJQvCdNSOZ9z75_uBMviJpj8HMB3J2_uly-4os0bodMURr-v2bm8hoIoas78lkRgN7m5DsAqIj_QGJdeRzMvP-MHrwAEskZgmYS2f9YMcpn0Mwboc98Y6An2YD6ZcbGAc4jn5lvUMSZwQ7oYtPi0eDGSM-u6unxfbd2-3Fh_L64_uri_PrEhStUykrrlDqRvHegOklNjXjohPAuRTUdEMDqtOoAJSQtAOsBlFBA00n1SC0OC3OjrZz8F8XjKmdbFwnMg7zUC3TknNFpZL3QHnV1Jmv7oGyRrA6LyyjL_9Bb_wSXP7yStV5aM3X3uxI5d3FGHBo52AnEw4to-0anPYYnDYHZ9WJVmXNizvnpZuw_6P4nZQM8CMQ81NeTvir9X9dfwKRZNC_</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Rosa, Luciano Pereira</creator><creator>da Silva, Francine Cristina</creator><creator>Nader, Sumaia Alves</creator><creator>Meira, Giselle Andrade</creator><creator>Viana, Magda Souza</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>C1K</scope></search><sort><creationdate>20141101</creationdate><title>In vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm laser and malachite green dye in Staphylococcus aureus biofilms arranged on compact and cancellous bone specimens</title><author>Rosa, Luciano Pereira ; da Silva, Francine Cristina ; Nader, Sumaia Alves ; Meira, Giselle Andrade ; Viana, Magda Souza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-4725e46952dacad4e98123b3c22430abf9c5b6e5cc5340bce7f37c9c9b45f363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis of variance</topic><topic>Animals</topic><topic>Anti-Infective Agents - 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radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosa, Luciano Pereira</creatorcontrib><creatorcontrib>da Silva, Francine Cristina</creatorcontrib><creatorcontrib>Nader, Sumaia Alves</creatorcontrib><creatorcontrib>Meira, Giselle Andrade</creatorcontrib><creatorcontrib>Viana, Magda Souza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosa, Luciano Pereira</au><au>da Silva, Francine Cristina</au><au>Nader, Sumaia Alves</au><au>Meira, Giselle Andrade</au><au>Viana, Magda Souza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm laser and malachite green dye in Staphylococcus aureus biofilms arranged on compact and cancellous bone specimens</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>29</volume><issue>6</issue><spage>1959</spage><epage>1965</epage><pages>1959-1965</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><coden>LMSCEZ</coden><abstract>The aim of this study was to evaluate the in vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm visible laser combined with malachite green (MG) dye in the inactivation of
Staphylococcus aureus
(ATCC 25923) biofilms formed within compact and cancellous bone specimens. Specimens of 80 compact bones and 80 cancellous bones were contaminated with a standard suspension of
S. aureus
and incubated for 14 days at 37 °C to allow for the formation of biofilms. The specimens were divided into the following groups (
n
= 10) according to the treatment conditions: PS−L − (control — no treatment), PS+L − (only MG for 5 min), PS−L + 90 (only laser irradiation for 90 s), PS−L + 180 (only laser irradiation for 180 s), PS−L + 300 (only laser irradiation for 300 s), APDT90 (APDT for 90 s), APDT180 (APDT for 180 s), and APDT300 (APDT for 300 s). The findings were statistically analyzed using an ANOVA 5 %. All of the experimental groups were significantly different from the control group for both the compact and cancellous bone specimens. The compact bone specimens that received APDT treatment (for either 90, 180, or 300 s) showed reductions in the log10 CFU/ml of
S. aureus
by a magnitude of 4 log10. Cancellous bone specimens treated with 300 s of APDT showed the highest efficacy, and these specimens had a reduction in
S. aureus
CFU/ml by a factor of 3 log10. APDT treatment using these proposed parameters in combination with MG was effective at inactivating
S. aureus
biofilms in compact and cancellous bone specimens.</abstract><cop>London</cop><pub>Springer London</pub><pmid>24935702</pmid><doi>10.1007/s10103-014-1613-5</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-8921 |
| ispartof | Lasers in medical science, 2014-11, Vol.29 (6), p.1959-1965 |
| issn | 0268-8921 1435-604X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analysis of variance Animals Anti-Infective Agents - chemistry Biofilms Biofilms - radiation effects Bone and Bones - drug effects Bone and Bones - microbiology Bone and Bones - radiation effects Bones Cattle Coloring Agents - chemistry Dentistry Effectiveness studies In vitro testing Irradiation Laser surgery Laser Therapy - methods Lasers Medicine Medicine & Public Health Optical Devices Optics Original Article Photochemotherapy - methods Photonics Quantum Optics Reduction Rosaniline Dyes - chemistry Staphylococcus aureus Staphylococcus aureus - radiation effects Therapy Tibia - drug effects Tibia - microbiology Tibia - radiation effects |
| title | In vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm laser and malachite green dye in Staphylococcus aureus biofilms arranged on compact and cancellous bone specimens |
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