Regioselective hydroxylation of 17β-estradiol by mutants of CYP102A1 from Bacillus megaterium

A large set of mutants of CYP102A1 from Bacillus megaterium have human cytochrome P450-like activities and the ability to metabolize a number of marketed drugs and steroids. Here, we tested whether the CYP102A1 mutants could be used to produce hydroxylated human metabolites of 17β-estradiol (E₂). A...

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Veröffentlicht in:	Biotechnology letters 2014-12, Vol.36 (12), p.2501-2506
Hauptverfasser:	Cha, Gun Su, Ryu, Sang Hoon, Ahn, Taeho, Yun, Chul-Ho
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied Microbiology Bacillus megaterium Bacillus megaterium - enzymology Bacillus megaterium - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biochemistry Bioconversion Biomedical and Life Sciences Biotechnology Biotransformation Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism drugs estradiol Estradiol - metabolism Hormones Human humans Hydroxylation industrial applications Life Sciences Metabolites Microbiology Mutagenesis Mutant Proteins - genetics Mutant Proteins - metabolism mutants NADPH-Ferrihemoprotein Reductase - genetics NADPH-Ferrihemoprotein Reductase - metabolism Original Research Paper steroid hormones Steroids Substrate Specificity
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