Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress
Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptid...
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| Veröffentlicht in: | Biotechnology letters 2014-12, Vol.36 (12), p.2559-2569 |
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| creator | Lv, Yonggang Hao, Xiaoying Sha, Yongqiang Yang, Li |
| description | Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm⁻² FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm⁻² FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering. |
| doi_str_mv | 10.1007/s10529-014-1625-z |
| format | Article |
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Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm⁻² FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm⁻² FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-014-1625-z</identifier><identifier>PMID: 25129046</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Apoptosis ; Applied Microbiology ; Biochemistry ; Biomechanical Phenomena ; Biomedical and Life Sciences ; Biophysics ; Biotechnology ; Bone marrow ; Bone Marrow - physiology ; cell proliferation ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Damage ; Fluid dynamics ; Fluid flow ; Fluid mechanics ; Fluids ; Insulin-Like Growth Factor I - metabolism ; Life Sciences ; Mesenchymal Stromal Cells - physiology ; Microbiology ; Original Research Paper ; Peptides ; Pretreatment ; Shear stress ; Stem cells ; synergism ; Synergistic effect ; Tissue engineering ; viability</subject><ispartof>Biotechnology letters, 2014-12, Vol.36 (12), p.2559-2569</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-f5b87427bd0407e35161222445f9774f523476020f3b5cb40c30dff6c3e3624a3</citedby><cites>FETCH-LOGICAL-c532t-f5b87427bd0407e35161222445f9774f523476020f3b5cb40c30dff6c3e3624a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-014-1625-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-014-1625-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25129046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Yonggang</creatorcontrib><creatorcontrib>Hao, Xiaoying</creatorcontrib><creatorcontrib>Sha, Yongqiang</creatorcontrib><creatorcontrib>Yang, Li</creatorcontrib><title>Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm⁻² FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm⁻² FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering.</description><subject>Apoptosis</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomechanical Phenomena</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Bone Marrow - physiology</subject><subject>cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Damage</subject><subject>Fluid dynamics</subject><subject>Fluid flow</subject><subject>Fluid mechanics</subject><subject>Fluids</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Life Sciences</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Microbiology</subject><subject>Original Research Paper</subject><subject>Peptides</subject><subject>Pretreatment</subject><subject>Shear stress</subject><subject>Stem cells</subject><subject>synergism</subject><subject>Synergistic effect</subject><subject>Tissue engineering</subject><subject>viability</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkU1rFTEUhoMo9lr9AW400I2b0eTka-5SSrVCQUG7DpnMydwp83FNMpRb8L-by9QiLqSrEM7zvufjJeQ1Z-85Y-ZD4kzBtmJcVlyDqu6ekA1XRlTaGP2UbEqBV0pu4YS8SOmGMbY1zDwnJ6A4bJnUG_LrW8Qc0eURp0xv-7yjI_qdm-aqi_Nt-Qbn8xzpBd3jPvct0n2cM_qcaDNPSEcXC1dECSe_O4xuoB6HIVHXuX5KmbZudB3S5kDDsPQtTTt0kabSNKWX5FlwQ8JX9-8puf508eP8srr6-vnL-ceryisBuQqqqY0E07RMMoNCcc0BQEoVtsbIoEBIoxmwIBrlG8m8YG0I2gsUGqQTp-Td6ltm_7lgynbs03FMN-G8JMu1BBB1LeERKIAud1f8ESivayiudUHP_kFv5iVOZecjZQwXhutC8ZXycU4pYrD72JcDHyxn9pi4XRO3JdjjIMreFc2be-elGbF9UPyJuACwAqmUpg7jX63_4_p2FQU3W9fFPtnr71AAxnjNwTDxGxPvvxs</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Lv, Yonggang</creator><creator>Hao, Xiaoying</creator><creator>Sha, Yongqiang</creator><creator>Yang, Li</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20141201</creationdate><title>Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress</title><author>Lv, Yonggang ; Hao, Xiaoying ; Sha, Yongqiang ; Yang, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-f5b87427bd0407e35161222445f9774f523476020f3b5cb40c30dff6c3e3624a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biomechanical Phenomena</topic><topic>Biomedical and Life Sciences</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>Bone Marrow - physiology</topic><topic>cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Damage</topic><topic>Fluid dynamics</topic><topic>Fluid flow</topic><topic>Fluid mechanics</topic><topic>Fluids</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Life Sciences</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Microbiology</topic><topic>Original Research Paper</topic><topic>Peptides</topic><topic>Pretreatment</topic><topic>Shear stress</topic><topic>Stem cells</topic><topic>synergism</topic><topic>Synergistic effect</topic><topic>Tissue engineering</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Yonggang</creatorcontrib><creatorcontrib>Hao, Xiaoying</creatorcontrib><creatorcontrib>Sha, Yongqiang</creatorcontrib><creatorcontrib>Yang, Li</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Yonggang</au><au>Hao, Xiaoying</au><au>Sha, Yongqiang</au><au>Yang, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>36</volume><issue>12</issue><spage>2559</spage><epage>2569</epage><pages>2559-2569</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm⁻² FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm⁻² FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>25129046</pmid><doi>10.1007/s10529-014-1625-z</doi><tpages>11</tpages></addata></record> |
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| ispartof | Biotechnology letters, 2014-12, Vol.36 (12), p.2559-2569 |
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| subjects | Apoptosis Applied Microbiology Biochemistry Biomechanical Phenomena Biomedical and Life Sciences Biophysics Biotechnology Bone marrow Bone Marrow - physiology cell proliferation Cell Proliferation - drug effects Cell Survival - drug effects Damage Fluid dynamics Fluid flow Fluid mechanics Fluids Insulin-Like Growth Factor I - metabolism Life Sciences Mesenchymal Stromal Cells - physiology Microbiology Original Research Paper Peptides Pretreatment Shear stress Stem cells synergism Synergistic effect Tissue engineering viability |
| title | Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress |
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