Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection
Zinc oxide (ZnO) nanostructures possessing flower-like morphology have been synthesised onto platinized silicon substrate by simple and economical hydrothermal method. The interaction of physically immobilized single stranded thiolated DNA (ss th-DNA) probe of N. meningitides onto the nanostructured...
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| Veröffentlicht in: | Biosensors & bioelectronics 2014-09, Vol.59, p.200-207 |
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| description | Zinc oxide (ZnO) nanostructures possessing flower-like morphology have been synthesised onto platinized silicon substrate by simple and economical hydrothermal method. The interaction of physically immobilized single stranded thiolated DNA (ss th-DNA) probe of N. meningitides onto the nanostructured ZnO (ZNF) matrix surface have been investigated using cyclic voltammetry (CV) and electrochemical impeadance spectroscopy (EIS). The electrochemical sensing response behaviour of the DNA bioelectrode (ss th-DNA/ZNF/Pt/Si) has been studied by both differential pulse voltammetric (DPV) as well as impedimetric techniques. The fabricated DNA biosensor can quantify wide range of the complementary target ss th-DNA in the range 5–240ngμl−1 with good linearity (R=0.98), high sensitivity (168.64μAng−1μlcm−2) and low detection limit of about 5ngμl−1. Results emphasise that the fabricated flower-like ZnO nanostructures offer a useful platform for the immobilization of DNA molecules and could be exploited for efficient detection of complementary target single stranded DNA corresponding to N. meningitides.
•DNA biosensor based on flower-like ZnO nanostructures for Meningitis detection has been reported.•ZnO nanostructures have been synthesized by very simple and economical hydrothermal method.•The fabricated DNA biosensor, insures a highly developed active surface for DNA immobilization.•Very high sensitivity, wide detection range of the target DNA along with long stability has been observed. |
| doi_str_mv | 10.1016/j.bios.2014.03.036 |
| format | Article |
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•DNA biosensor based on flower-like ZnO nanostructures for Meningitis detection has been reported.•ZnO nanostructures have been synthesized by very simple and economical hydrothermal method.•The fabricated DNA biosensor, insures a highly developed active surface for DNA immobilization.•Very high sensitivity, wide detection range of the target DNA along with long stability has been observed.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.03.036</identifier><identifier>PMID: 24727606</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Bacteria ; Base Sequence ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensor ; Biosensors ; Biotechnology ; Deoxyribonucleic acid ; DNA ; DNA Probes - chemistry ; DNA Probes - genetics ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; DNA, Single-Stranded - chemistry ; DNA, Single-Stranded - genetics ; Economics ; Electrochemical impedance spectroscopy ; Electrochemical Techniques - instrumentation ; Equipment Design ; Fundamental and applied biological sciences. Psychology ; Hydrothermal method ; Limit of Detection ; Linearity ; Meningitis ; Meningitis, Bacterial - diagnosis ; Meningitis, Bacterial - microbiology ; Nanostructure ; Nanostructures - chemistry ; Nanostructures - ultrastructure ; Neisseria meningitidis - genetics ; Neisseria meningitidis - isolation & purification ; Zinc oxide ; Zinc Oxide - chemistry</subject><ispartof>Biosensors & bioelectronics, 2014-09, Vol.59, p.200-207</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-f60d87037b01326ea175e1687f7415ceb13ee7a671b7d7acc167661c8093aca23</citedby><cites>FETCH-LOGICAL-c518t-f60d87037b01326ea175e1687f7415ceb13ee7a671b7d7acc167661c8093aca23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566314002176$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28541186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24727606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tak, Manvi</creatorcontrib><creatorcontrib>Gupta, Vinay</creatorcontrib><creatorcontrib>Tomar, Monika</creatorcontrib><title>Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Zinc oxide (ZnO) nanostructures possessing flower-like morphology have been synthesised onto platinized silicon substrate by simple and economical hydrothermal method. The interaction of physically immobilized single stranded thiolated DNA (ss th-DNA) probe of N. meningitides onto the nanostructured ZnO (ZNF) matrix surface have been investigated using cyclic voltammetry (CV) and electrochemical impeadance spectroscopy (EIS). The electrochemical sensing response behaviour of the DNA bioelectrode (ss th-DNA/ZNF/Pt/Si) has been studied by both differential pulse voltammetric (DPV) as well as impedimetric techniques. The fabricated DNA biosensor can quantify wide range of the complementary target ss th-DNA in the range 5–240ngμl−1 with good linearity (R=0.98), high sensitivity (168.64μAng−1μlcm−2) and low detection limit of about 5ngμl−1. Results emphasise that the fabricated flower-like ZnO nanostructures offer a useful platform for the immobilization of DNA molecules and could be exploited for efficient detection of complementary target single stranded DNA corresponding to N. meningitides.
•DNA biosensor based on flower-like ZnO nanostructures for Meningitis detection has been reported.•ZnO nanostructures have been synthesized by very simple and economical hydrothermal method.•The fabricated DNA biosensor, insures a highly developed active surface for DNA immobilization.•Very high sensitivity, wide detection range of the target DNA along with long stability has been observed.</description><subject>Bacteria</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Probes - chemistry</subject><subject>DNA Probes - genetics</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>DNA, Single-Stranded - genetics</subject><subject>Economics</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Equipment Design</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrothermal method</subject><subject>Limit of Detection</subject><subject>Linearity</subject><subject>Meningitis</subject><subject>Meningitis, Bacterial - diagnosis</subject><subject>Meningitis, Bacterial - microbiology</subject><subject>Nanostructure</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - ultrastructure</subject><subject>Neisseria meningitidis - genetics</subject><subject>Neisseria meningitidis - isolation & purification</subject><subject>Zinc oxide</subject><subject>Zinc Oxide - chemistry</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi1E1S5tX4ADygWJS7YzdmJnJS5VSwGpai_tpRfLcSbgJbGLnRT17XG0C9wqpLF88Df_WN8w9hZhjYDybLtuXUhrDlitQeSSr9gKGyXKiov6NVvBppZlLaU4Ym9S2gKAwg0csiNeKa4kyBXrrobwi2I5uB9UPPjbwhsf0hRnO82RitYk6goayE4x2O80OmuG4vLmvFhGk08hFn0-rbETRZffRvLOf3OTS0VHU-5zwZ-wg94MiU739zG7v_p0d_GlvL79_PXi_Lq0NTZT2UvoGgVCtYCCSzKoakLZqF5VWFtqURApIxW2qlPGWpRKSrQNbISxhotj9mGX-xjDz5nSpEeXLA2D8RTmpFFWnGNTgfwPlKtNnV1iRvkOtTGkFKnXj9GNJj5rBL0sQm_1YkMvi9Agci357_b5cztS97flj_kMvN8DJmWnfTTeuvSPa-oKsVm4jzuOsrgnR1En68hb6lzMdnUX3Ev_-A1Eb6bJ</recordid><startdate>20140915</startdate><enddate>20140915</enddate><creator>Tak, Manvi</creator><creator>Gupta, Vinay</creator><creator>Tomar, Monika</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20140915</creationdate><title>Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection</title><author>Tak, Manvi ; Gupta, Vinay ; Tomar, Monika</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-f60d87037b01326ea175e1687f7415ceb13ee7a671b7d7acc167661c8093aca23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacteria</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Probes - chemistry</topic><topic>DNA Probes - genetics</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>DNA, Single-Stranded - genetics</topic><topic>Economics</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Equipment Design</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrothermal method</topic><topic>Limit of Detection</topic><topic>Linearity</topic><topic>Meningitis</topic><topic>Meningitis, Bacterial - diagnosis</topic><topic>Meningitis, Bacterial - microbiology</topic><topic>Nanostructure</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - ultrastructure</topic><topic>Neisseria meningitidis - genetics</topic><topic>Neisseria meningitidis - isolation & purification</topic><topic>Zinc oxide</topic><topic>Zinc Oxide - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tak, Manvi</creatorcontrib><creatorcontrib>Gupta, Vinay</creatorcontrib><creatorcontrib>Tomar, Monika</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tak, Manvi</au><au>Gupta, Vinay</au><au>Tomar, Monika</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2014-09-15</date><risdate>2014</risdate><volume>59</volume><spage>200</spage><epage>207</epage><pages>200-207</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Zinc oxide (ZnO) nanostructures possessing flower-like morphology have been synthesised onto platinized silicon substrate by simple and economical hydrothermal method. The interaction of physically immobilized single stranded thiolated DNA (ss th-DNA) probe of N. meningitides onto the nanostructured ZnO (ZNF) matrix surface have been investigated using cyclic voltammetry (CV) and electrochemical impeadance spectroscopy (EIS). The electrochemical sensing response behaviour of the DNA bioelectrode (ss th-DNA/ZNF/Pt/Si) has been studied by both differential pulse voltammetric (DPV) as well as impedimetric techniques. The fabricated DNA biosensor can quantify wide range of the complementary target ss th-DNA in the range 5–240ngμl−1 with good linearity (R=0.98), high sensitivity (168.64μAng−1μlcm−2) and low detection limit of about 5ngμl−1. Results emphasise that the fabricated flower-like ZnO nanostructures offer a useful platform for the immobilization of DNA molecules and could be exploited for efficient detection of complementary target single stranded DNA corresponding to N. meningitides.
•DNA biosensor based on flower-like ZnO nanostructures for Meningitis detection has been reported.•ZnO nanostructures have been synthesized by very simple and economical hydrothermal method.•The fabricated DNA biosensor, insures a highly developed active surface for DNA immobilization.•Very high sensitivity, wide detection range of the target DNA along with long stability has been observed.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>24727606</pmid><doi>10.1016/j.bios.2014.03.036</doi><tpages>8</tpages></addata></record> |
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| subjects | Bacteria Base Sequence Biological and medical sciences Biosensing Techniques - instrumentation Biosensor Biosensors Biotechnology Deoxyribonucleic acid DNA DNA Probes - chemistry DNA Probes - genetics DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics Economics Electrochemical impedance spectroscopy Electrochemical Techniques - instrumentation Equipment Design Fundamental and applied biological sciences. Psychology Hydrothermal method Limit of Detection Linearity Meningitis Meningitis, Bacterial - diagnosis Meningitis, Bacterial - microbiology Nanostructure Nanostructures - chemistry Nanostructures - ultrastructure Neisseria meningitidis - genetics Neisseria meningitidis - isolation & purification Zinc oxide Zinc Oxide - chemistry |
| title | Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection |
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